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BMC Musculoskeletal Disorders
Published in: BMC Musculoskeletal Disorders 1/2016

Open Access 01-12-2016 | Research article

Local pamidronate influences fracture healing in a rodent femur fracture model: an experimental study

Authors: Leif Menzdorf, Matthias Weuster, Tim Klüter, Stefan Brüggemann, Peter Behrendt, Stefanie Fitchen-Oestern, Deike Varoga, Andreas Seekamp, Nicolai Purcz, Claus C Glueer, Thomas Pufe, Sebastian Lippross

Published in: BMC Musculoskeletal Disorders | Issue 1/2016

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Abstract

Background

Bisphosphonates are a main component in the therapy of osteoporosis and other bone resorptive diseases. Previous studies have shown a positive effect of systemically applied bisphosphonates on fracture healing. Nevertheless high doses are related to side effects like osteonecrosis of the jaw, nephrotoxis and gastrointestinal symptoms. In this study we investigated the effect of locally applied pamidronate on fracture healing.

Methods

In a rodent model a simple femur fracture was set in female Wistar rats. We performed intramedullary fixation of the fracture and placed a collagen matrix around the fracture area. One group was treated with pamidronate, the other group with placebo via the matrix.
To investigate the volume and quality of the callus we used micro-CT (μCT) and histology after 14 and 28 days.

Results

Our results show a positive influence of local applied pamidronate on callus volume. After 14 days an insignificant increase of callus volume in the treated animals was seen. 28 days after trauma the increase of callus volume in the treatment group was significantly higher in comparison to the control group. Osteonecrosis was not seen.

Conclusions

Locally applied bisphosphonates increase the callus volume in fracture healing.
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Metadata
Title
Local pamidronate influences fracture healing in a rodent femur fracture model: an experimental study
Authors
Leif Menzdorf
Matthias Weuster
Tim Klüter
Stefan Brüggemann
Peter Behrendt
Stefanie Fitchen-Oestern
Deike Varoga
Andreas Seekamp
Nicolai Purcz
Claus C Glueer
Thomas Pufe
Sebastian Lippross
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Musculoskeletal Disorders / Issue 1/2016
Electronic ISSN: 1471-2474
DOI
https://doi.org/10.1186/s12891-016-1113-9

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