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Annals of Surgical Oncology
Published in: Annals of Surgical Oncology 12/2016

01-11-2016 | Colorectal Cancer

Immature Colon Carcinoma Transcript-1 (ICT1) Expression Correlates with Unfavorable Prognosis and Survival in Patients with Colorectal Cancer

Authors: Xinyuan Lao, PhD, Qingyang Feng, MD, Guodong He, MD, PhD, Meiling Ji, MA, Dexiang Zhu, MD, PhD, Pingping Xu, MD, PhD, Wentao Tang, MD, PhD, Jianmin Xu, MD, PhD, Xinyu Qin, MD, PhD

Published in: Annals of Surgical Oncology | Issue 12/2016

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Abstract

Background

Colorectal cancer (CRC) is the third leading cause of death from cancer worldwide. Immature colon carcinoma transcript-1 (ICT1) has been reported to be correlated with lung cancer; however, whether ICT1 is a functional gene in CRC, as well as the molecular mechanism underlying ICT1 mediation of colorectal-tumor formation, remains unknown.

Methods

The expression of ICT1 was firstly determined by using immunohistochemistry in 861 CRC specimens. The correlation of ICT1 expression with clinicopathological parameters and the survival rate was analyzed. Furthermore, we investigated the effect of ICT1 silencing on CRC cell proliferation and migration by MTT, colony formation, flow cytometry, and transwell in vitro.

Results

ICT1 is highly expressed in a cohort of human CRCs, and that higher ICT1 expression may lead to reduced overall survival rate of CRC. Likewise, ICT1 silencing lowered the cell viability through cell-cycle arrest, inhibited cell migration, and induced apoptosis in CRC. We further revealed a novel mechanism in which ICT1 promoted CRC growth via the intracellular AMPK, SAPK/JNK, and PARP signaling pathways.

Conclusions

Our data showed that ICT1 could be an important target for CRC diagnosis and treatment.
Appendix
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Metadata
Title
Immature Colon Carcinoma Transcript-1 (ICT1) Expression Correlates with Unfavorable Prognosis and Survival in Patients with Colorectal Cancer
Authors
Xinyuan Lao, PhD
Qingyang Feng, MD
Guodong He, MD, PhD
Meiling Ji, MA
Dexiang Zhu, MD, PhD
Pingping Xu, MD, PhD
Wentao Tang, MD, PhD
Jianmin Xu, MD, PhD
Xinyu Qin, MD, PhD
Publication date
01-11-2016
Publisher
Springer International Publishing
Published in
Annals of Surgical Oncology / Issue 12/2016
Print ISSN: 1068-9265
Electronic ISSN: 1534-4681
DOI
https://doi.org/10.1245/s10434-016-5305-1

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