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Annals of Surgical Oncology
Published in: Annals of Surgical Oncology 2/2016

01-02-2016 | Gastrointestinal Oncology

Abdominal Infection Suppresses the Number and Activity of Intrahepatic Natural Killer Cells and Promotes Tumor Growth in a Murine Liver Metastasis Model

Authors: Yusuke Matsumoto, MD, PhD, Hironori Tsujimoto, MD, PhD, Satoshi Ono, MD, PhD, Nariyoshi Shinomiya, MD, PhD, Hiromi Miyazaki, PhD, Shuichi Hiraki, MD, PhD, Risa Takahata, MD, PhD, Kazumichi Yoshida, MD, PhD, Daizoh Saitoh, MD, PhD, Takao Yamori, PhD, Junji Yamamoto, MD, PhD, Kazuo Hase, MD, PhD

Published in: Annals of Surgical Oncology | Special Issue 2/2016

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Abstract

Background

Increasing evidence suggests that postoperative infection is associated with poorer long-term outcome in various malignancies. However, the mechanism of poor prognosis induced by postoperative infection has not been clearly explained. We sought to determine whether abdominal infection promotes cancer metastases in a murine liver metastasis model, and to investigate the role of liver natural killer (NK) cells on antitumor immunity during abdominal infection.

Methods

Female BALB/c (8–10 weeks old) mice were inoculated with NL-17 colon cancer cells into the spleen and then subjected to abdominal infection induced by cecal ligation and puncture (CLP) or sham treatment. The extent of liver metastases and cytokine production in the serum and liver were investigated. Cell fraction and cytotoxic activities of liver mononuclear cells (MNCs) were elucidated.

Results

CLP mice had poorer survival and their serum levels of IL-6, -10, and -12p70 were significantly elevated on day 1 compared with sham-treated and control mice. No obvious differences in cytokine levels of the liver homogenates were identified among the three groups, except IL-12p70 levels in CLP mice on day 7 significantly decreased. The cytotoxic activities of liver MNCs were significantly suppressed in CLP mice soon after tumor inoculation. Flow cytometry revealed a decrease in NK cells in the liver and perforin and granzyme B expression levels.

Conclusions

Abdominal infection promoted liver metastases in a murine liver metastasis model, which may be partially caused by a decrease in the number and activity of NK cells during abdominal infection.
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Metadata
Title
Abdominal Infection Suppresses the Number and Activity of Intrahepatic Natural Killer Cells and Promotes Tumor Growth in a Murine Liver Metastasis Model
Authors
Yusuke Matsumoto, MD, PhD
Hironori Tsujimoto, MD, PhD
Satoshi Ono, MD, PhD
Nariyoshi Shinomiya, MD, PhD
Hiromi Miyazaki, PhD
Shuichi Hiraki, MD, PhD
Risa Takahata, MD, PhD
Kazumichi Yoshida, MD, PhD
Daizoh Saitoh, MD, PhD
Takao Yamori, PhD
Junji Yamamoto, MD, PhD
Kazuo Hase, MD, PhD
Publication date
01-02-2016
Publisher
Springer International Publishing
Published in
Annals of Surgical Oncology / Issue Special Issue 2/2016
Print ISSN: 1068-9265
Electronic ISSN: 1534-4681
DOI
https://doi.org/10.1245/s10434-015-4466-7

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