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01-07-2012 | Translational Research and Biomarkers

Claudin-4 Expression Predicts Survival in Pancreatic Ductal Adenocarcinoma

Authors: Kosuke Tsutsumi, MD, PhD, Norihiro Sato, MD, PhD, Reiko Tanabe, MD, Kazuhiro Mizumoto, MD, PhD, Katsuya Morimatsu, MD, Tadashi Kayashima, MD, Hayato Fujita, MD, PhD, Kenoki Ohuchida, MD, PhD, Takao Ohtsuka, MD, PhD, Shunichi Takahata, MD, PhD, Masafumi Nakamura, MD, PhD, Masao Tanaka, MD, PhD, FACS

Published in: Annals of Surgical Oncology | Special Issue 3/2012

Abstract

Background

Identification of prognostic markers would be useful in the clinical management of patients with pancreatic ductal adenocarcinoma (PDAC). The clinical relevance of claudin-4 (CLDN4), recently identified as overexpressed in PDAC, is unknown.

Methods

Using quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR), we analyzed CLDN4 mRNA expression in a panel of 9 pancreatic cancer cell lines and formalin-fixed paraffin-embedded (FFPE) tissues from 100 patients with PDAC. The CLDN4 expression levels were then correlated with clinicopathological variables and patient outcome. We also performed immunohistochemical analysis in 20 FFPE samples of PDAC to investigate the expression of CLDN4 protein.

Results

Increased expression of CLDN4 was confirmed in all the pancreatic cancer cell lines tested compared with normal ductal epithelial cells and fibroblasts. We found that low expression of CLDN4 was significantly associated with shorter survival in patients with PDAC (hazard ratio; 1.362, 95% confidence interval; 1.011–1.873, P = 0.0419). Patients with high CLDN4 expression survived longer for a median of 63.0 months, compared with 14.7 months in patients with low CLDN4 expression (P = 0.0067). In immunohistochemical analysis, the level of CLDN4 mRNA expression was significantly correlated with the expression of CLDN4 protein (P = 0.0168).

Conclusion

Increased expression of CLDN4 mRNA predicts better prognosis in PDAC.

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Title: Claudin-4 Expression Predicts Survival in Pancreatic Ductal Adenocarcinoma
Authors: Kosuke Tsutsumi, MD, PhD
Norihiro Sato, MD, PhD
Reiko Tanabe, MD
Kazuhiro Mizumoto, MD, PhD
Katsuya Morimatsu, MD
Tadashi Kayashima, MD
Hayato Fujita, MD, PhD
Kenoki Ohuchida, MD, PhD
Takao Ohtsuka, MD, PhD
Shunichi Takahata, MD, PhD
Masafumi Nakamura, MD, PhD
Masao Tanaka, MD, PhD, FACS
Publication date: 01-07-2012
Publisher: Springer-Verlag
Published in: Annals of Surgical Oncology / Issue Special Issue 3/2012
Print ISSN: 1068-9265
Electronic ISSN: 1534-4681
DOI: https://doi.org/10.1245/s10434-011-1970-2

At a glance: The STEP trials

Springer Medicine

Claudin-4 Expression Predicts Survival in Pancreatic Ductal Adenocarcinoma

Abstract

Background

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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