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Annals of Surgical Oncology
Published in: Annals of Surgical Oncology 1/2011

01-01-2011 | Translational Research and Biomarkers

STC2: A Predictive Marker for Lymph Node Metastasis in Esophageal Squamous-Cell Carcinoma

Authors: Yoshiaki Kita, MD, PhD, Koshi Mimori, MD, PhD, Masaaki Iwatsuki, MD, PhD, Takehiko Yokobori, MD, PhD, Keisuke Ieta, MD, PhD, Fumiaki Tanaka, MD, PhD, Hideshi Ishii, MD, PhD, Hiroshi Okumura, MD, PhD, Shoji Natsugoe, MD, PhD, Masaki Mori, MD, PhD, FACS

Published in: Annals of Surgical Oncology | Issue 1/2011

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Abstract

Background

We sought to identify genes associated with the progression and metastasis of esophageal squamous-cell cancer by comparing the expression profiles of normal, primary cancer, and metastatic cancer cells isolated with laser microdissection.

Methods

Oligo microarray analysis identified several lymph node-specific, metastasis-related genes. STC2 (stanniocalcin 2), which was overexpressed in esophageal cancer cases, was chosen for further characterization. Quantitative reverse transcriptase-polymerase chain reaction and immunohistochemistry were used to explore the clinicopathologic significance of STC2 expression status in 70 cases. Additionally, the functional role of STC2 in esophageal cancer was studied by the attenuation of STC2 in an esophageal cancer cell line.

Results

Laser microdissection and oligo microarray analysis identified 63 candidate genes. Among them, STC2 showed higher expression in cancer tissue than in corresponding normal tissue (P < 0.001). STC2 expression was significantly correlated with lymph node metastasis, lymphatic invasion, and distant metastasis (P = 0.005, 0.007, and 0.038, respectively). Patients whose tumors had high STC2 expression had a worse 5-year survival rate than patients whose tumors had a low STC2 expression level (P = 0.016). STC2 transfected cells had a significantly higher proliferation rate than control cells (P < 0.001). Additionally, STC2 transfected cells were more invasive in vitro (P < 0.001) than control cells. These findings were validated by means of RNA interference assays.

Conclusions

We identified lymph node-specific, metastasis-related genes in esophageal cancer cells. One of these, STC2, may be associated with lymph node metastasis, making it a potential prognostic marker for esophageal cancer patients.
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Metadata
Title
STC2: A Predictive Marker for Lymph Node Metastasis in Esophageal Squamous-Cell Carcinoma
Authors
Yoshiaki Kita, MD, PhD
Koshi Mimori, MD, PhD
Masaaki Iwatsuki, MD, PhD
Takehiko Yokobori, MD, PhD
Keisuke Ieta, MD, PhD
Fumiaki Tanaka, MD, PhD
Hideshi Ishii, MD, PhD
Hiroshi Okumura, MD, PhD
Shoji Natsugoe, MD, PhD
Masaki Mori, MD, PhD, FACS
Publication date
01-01-2011
Publisher
Springer-Verlag
Published in
Annals of Surgical Oncology / Issue 1/2011
Print ISSN: 1068-9265
Electronic ISSN: 1534-4681
DOI
https://doi.org/10.1245/s10434-010-1271-1

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