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Annals of Surgical Oncology
Published in: Annals of Surgical Oncology 10/2005

01-10-2005

Interleukin 8 in Human Hepatocellular Carcinoma Correlates With Cancer Cell Invasion of Vessels But Not With Tumor Angiogenesis

Authors: Fumitake Kubo, MD, Shinichi Ueno, MD, PhD, Kiyokazu Hiwatashi, MD, Masahiko Sakoda, MD, Koichi Kawaida, MD, Kensuke Nuruki, MD, Takashi Aikou, MD, PhD

Published in: Annals of Surgical Oncology | Issue 10/2005

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Abstract

Background

Angiogenic factor seems necessary for the development of hepatocellular carcinoma (HCC), which is a hypervascular malignancy. This study examined the expression of interleukin (IL)-8, a potent angiogenic factor, in HCC samples.

Methods

We measured IL−8 expression by using reverse transcriptase-polymerase chain reaction in clinical HCC tissues from 45 patients who underwent surgical resection. We then assessed correlations between IL−8 expression and microvessel growth or clinicopathologic factors. We also elucidated the in vitro effect of IL−8 on HepG2 development by using fluorometric assays of proliferation, chemotaxis, and invasion.

Results

The expression of IL−8 did not significantly correlate with the microvessel count in HCC tissues, but the incidence of microscopic vessel invasion was significantly higher in IL−8–positive than in IL−8–negative tissues. Thus, more IL−8 was expressed in HCCs at pathologic stage III/IV than in those at stage I/II. Assays in vitro showed that IL−8 stimulates HepG2 chemotactic and invasive activities rather than cell proliferation.

Conclusions

The expression of IL−8 in human HCC has more relevance to metastatic potential, such as vessel invasion, than to angiogenesis or cell proliferation.
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Metadata
Title
Interleukin 8 in Human Hepatocellular Carcinoma Correlates With Cancer Cell Invasion of Vessels But Not With Tumor Angiogenesis
Authors
Fumitake Kubo, MD
Shinichi Ueno, MD, PhD
Kiyokazu Hiwatashi, MD
Masahiko Sakoda, MD
Koichi Kawaida, MD
Kensuke Nuruki, MD
Takashi Aikou, MD, PhD
Publication date
01-10-2005
Publisher
Springer-Verlag
Published in
Annals of Surgical Oncology / Issue 10/2005
Print ISSN: 1068-9265
Electronic ISSN: 1534-4681
DOI
https://doi.org/10.1245/ASO.2005.07.015

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