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01-02-2016 | Colorectal Cancer

OSNA-Assisted Molecular Staging in Colorectal Cancer: A Prospective Multicenter Trial in Japan

Authors: Hirofumi Yamamoto, MD, PhD, Naohiro Tomita, MD, PhD, Masafumi Inomata, MD, Tomohisa Furuhata, MD, PhD, Yasuhiro Miyake, MD, PhD, Shingo Noura, MD, PhD, Takeshi Kato, MD, PhD, Kohei Murata, MD, PhD, Shigeoki Hayashi, MD, PhD, Seiji Igarashi, MD, PhD, Michio Itabashi, MD, PhD, Shingo Kameoka, MD, PhD, Nariaki Matsuura, MD, PhD

Published in: Annals of Surgical Oncology | Issue 2/2016

Abstract

Background

We previously reported that the one-step nucleic acid amplification (OSNA) assay provided a judgment performance for colorectal cancer equivalent to a 2-mm-interval histopathological examination of lymph nodes (concordance 97.1 %, n = 385 lymph nodes). In this prospective multicenter study, we uncovered an OSNA-assisted pathology to detect lymph node metastasis.

Methods

A total of 204 (50 stage I, 74 stage II, and 80 stage III) colorectal cancer patients. All 4324 lymph nodes were examined by the standard histology (one-slice H&E staining) and 1925 lymph nodes (44.5 %) of them were also subject to the OSNA analysis.

Results

The concordance rate between 1 slice hematoxylin/eosin and OSNA assay was 95.7 % (1,842/1925 lymph nodes). The sensitivity and specificity of the OSNA assay were 86.2 % (125/145) and 96.5 % (1717/1780), respectively. Among 124 node-negative patients (pN0), the respective upstaging rates of pStages I, IIA, IIB, and IIC were 2.0 % (1/50), 17.7 % (11/62), 12.5 % (1/8), and 25 % (1/4). OSNA-positive patients had deeper invasion to the colonic wall and severe lymphatic invasion (P = 0.048 and P = 0.004, respectively). The sum of the quantitative results of OSNA and total tumor load increased as the number of metastasized lymph nodes increased: 1550 copies/μL in pN0, 24,050 copies/μL in pN1, and 90,600 copies/μL in pN2.

Conclusions

The present study on colorectal cancer provided fundamental data regarding OSNA-assisted pathology of lymph node metastasis in Japan.

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Title: OSNA-Assisted Molecular Staging in Colorectal Cancer: A Prospective Multicenter Trial in Japan
Authors: Hirofumi Yamamoto, MD, PhD
Naohiro Tomita, MD, PhD
Masafumi Inomata, MD
Tomohisa Furuhata, MD, PhD
Yasuhiro Miyake, MD, PhD
Shingo Noura, MD, PhD
Takeshi Kato, MD, PhD
Kohei Murata, MD, PhD
Shigeoki Hayashi, MD, PhD
Seiji Igarashi, MD, PhD
Michio Itabashi, MD, PhD
Shingo Kameoka, MD, PhD
Nariaki Matsuura, MD, PhD
Publication date: 01-02-2016
Publisher: Springer US
Published in: Annals of Surgical Oncology / Issue 2/2016
Print ISSN: 1068-9265
Electronic ISSN: 1534-4681
DOI: https://doi.org/10.1245/s10434-015-4880-x

Keynote webinar | Spotlight on medication adherence

Springer Medicine

OSNA-Assisted Molecular Staging in Colorectal Cancer: A Prospective Multicenter Trial in Japan

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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