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01-08-2012 | Hepatobiliary Tumors

Intratumoral IL-17⁺ Cells and Neutrophils show Strong Prognostic Significance in Intrahepatic Cholangiocarcinoma

Authors: Fang-Ming Gu, MD, Qiang Gao, MD, Guo-Ming Shi, MD, Xin Zhang, MD, Jiping Wang, MD, Jia-Hao Jiang, MD, Xiao-Ying Wang, MD, Ying-Hong Shi, MD, Zhen-Bin Ding, MD, Jia Fan, MD, Jian Zhou, MD

Published in: Annals of Surgical Oncology | Issue 8/2012

Abstract

Background

Inflammatory reactions at a tumor site have both detrimental and beneficial effects on tumor progression. This study was designed to assess the clinical significance of tumor-infiltrating inflammatory cells in patients with intrahepatic cholangiocarcinoma (ICC).

Methods

A total of 123 consecutive ICC patients who underwent curative resection were enrolled. Tissue microarray and immunohistochemistry were used to analyze the distribution and clinical relevance of IL-17⁺, FOXP3⁺, CD8⁺, CD66b⁺ cells, and microvessel density (CD34) in different microanatomical areas.

Results

IL-17⁺ cells, FOXP3⁺ lymphocytes, CD66b⁺ neutrophils, and microvessels were enriched predominantly in intratumor (IT) area, whereas CD8⁺ lymphocytes were most abundant in tumor invasive front. On univariate analyses, increasing IL-17 _IT ⁺ and neutrophils_IT were significantly associated with worse patient survival. Multivariate analyses revealed that IL-17 _IT ⁺ (hazard ratio [HR] = 1.59; 95% confidence interval [CI], 1.05–2.41; P = 0.028), neutrophils_IT (HR = 1.76; 95% CI, 1.16–2.65; P = 0.007), and their combination (HR 2.8; 95% CI 1.72–4.57; P < 0.001) were independent prognostic factors, which were superior to conventional clinicopathologic features, such as intrahepatic metastasis and TNM stage. IL-17 _IT ⁺ significantly correlated with the presence of lymph node metastasis, intrahepatic metastasis, and advanced stages, whereas neutrophils_IT correlated with the presence of vascular invasion. In addition, significant positive correlations were detected among densities of IL-17⁺ cells, neutrophils, and microvessel density.

Conclusions

Our data suggested that intratumor IL-17⁺ cells, neutrophils are novel, powerful predictors of prognosis in patients with ICC.

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Title: Intratumoral IL-17+ Cells and Neutrophils show Strong Prognostic Significance in Intrahepatic Cholangiocarcinoma
Authors: Fang-Ming Gu, MD
Qiang Gao, MD
Guo-Ming Shi, MD
Xin Zhang, MD
Jiping Wang, MD
Jia-Hao Jiang, MD
Xiao-Ying Wang, MD
Ying-Hong Shi, MD
Zhen-Bin Ding, MD
Jia Fan, MD
Jian Zhou, MD
Publication date: 01-08-2012
Publisher: Springer-Verlag
Published in: Annals of Surgical Oncology / Issue 8/2012
Print ISSN: 1068-9265
Electronic ISSN: 1534-4681
DOI: https://doi.org/10.1245/s10434-012-2268-8

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Intratumoral IL-17⁺ Cells and Neutrophils show Strong Prognostic Significance in Intrahepatic Cholangiocarcinoma

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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