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01-04-2012 | Translational Research and Biomarkers

Absence of CD71 Transferrin Receptor Characterizes Human Gastric Adenosquamous Carcinoma Stem Cells

Authors: Masahisa Ohkuma, MD, Naotsugu Haraguchi, MD, PhD, Hideshi Ishii, MD, PhD, Koshi Mimori, MD, PhD, Fumiaki Tanaka, MD, PhD, Ho Min Kim, MD, Miho Shimomura, MD, Hajime Hirose, MD, Katsuhiko Yanaga, MD, PhD, Masaki Mori, MD, PhD

Published in: Annals of Surgical Oncology | Issue 4/2012

Abstract

Background

Although the importance of cancer stem cells (CSCs) in overcoming resistance to therapy and metastasis has recently been reported, the role of CSCs in gastric cancer remains to be elucidated.

Methods

MKN-1 cells were used to study markers of CSCs in gastric adenosquamous carcinoma, as these cells are suitable for determining multidifferentiation ability. Changes in expression of CD44, CD49f, CD133, and CD71 following 5-fluorouracil (5-FU) treatment were assessed.

Results

After 5-FU treatment, only the CD71⁻ fraction was significantly increased. Investigation of CD71 indicated that the CD71⁻ cell fraction was present in the G1/G0 cell cycle phase and showed high resistance to the anticancer agent 5-FU. Limiting dilution and serial transplantation assays revealed the CD71⁻ cell fraction to have higher tumorigenicity than the CD71⁺ cell fraction. The CD71⁻ cell fraction showed multipotency to adenocarcinoma and squamous cell carcinoma. A three-dimensional (3D) invasion assay and immunohistochemical analysis showed CD71⁻ cells to be highly invasive and to exist in the invasive fronts of cancer foci.

Conclusion

The present study suggests that use of CD71⁻ as a marker for adenosquamous carcinoma may provide a useful model for studying CSCs.

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Title: Absence of CD71 Transferrin Receptor Characterizes Human Gastric Adenosquamous Carcinoma Stem Cells
Authors: Masahisa Ohkuma, MD
Naotsugu Haraguchi, MD, PhD
Hideshi Ishii, MD, PhD
Koshi Mimori, MD, PhD
Fumiaki Tanaka, MD, PhD
Ho Min Kim, MD
Miho Shimomura, MD
Hajime Hirose, MD
Katsuhiko Yanaga, MD, PhD
Masaki Mori, MD, PhD
Publication date: 01-04-2012
Publisher: Springer-Verlag
Published in: Annals of Surgical Oncology / Issue 4/2012
Print ISSN: 1068-9265
Electronic ISSN: 1534-4681
DOI: https://doi.org/10.1245/s10434-011-1739-7

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Absence of CD71 Transferrin Receptor Characterizes Human Gastric Adenosquamous Carcinoma Stem Cells

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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