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01-06-2011 | Translational Research and Biomarkers

Nicotine Enhances Colon Cancer Cell Migration by Induction of Fibronectin

Authors: Po-Li Wei, MD, Li-Jen Kuo, MD, Ming-Te Huang, MD, Wen-Chien Ting, MD, Yuan-Soon Ho, PhD, Weu Wang, MD, Jane An, MD, Yu-Jia Chang, PhD

Published in: Annals of Surgical Oncology | Issue 6/2011

Abstract

Background

Long-term cigarette smoking increases the risk of colorectal cancer mortality. Tobacco’s addictive toxin, nicotine, was reported to increase DNA synthesis of colon cancer cells. Because metastasis is the major cause of cancer death, the influence of nicotine on the migration of colon cancer cells remains to be determined.

Methods

The influence of nicotine on the migration of colon cancer cells was evaluated using transwell assay. Nicotine receptor-mediated migration was studied by using both inhibitors and small interfering RNA (siRNA). The role of COX-2 signal was studied using pharmacological inhibitors. The expression of epithelial mesenchymal transition (EMT) marker and COX-2 signal was evaluated using real-time polymerase chain reaction (PCR).

Results

Nicotine enhanced DLD-1 and SW480 cell migration in a dose-dependent manner. We used inhibitors and siRNA to demonstrate that α7-nAChR mediates nicotine-enhanced colon cancer cell migration and upregulates fibronectin expression, which is involved in nicotine-enhanced migration. Furthermore, COX-2 signal was induced by nicotine treatment and is involved in nicotine-enhanced fibronectin expression.

Conclusions

Nicotine, tobacco’s additive toxin, enhances colon cancer metastasis through α7-nAChR and fibronectin—a mesenchymal marker for epithelial mesenchymal transition. Furthermore, COX-2 signal was involved in the induction of fibronectin. Therefore, smoking may play role in the progression of colon cancer.

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Title: Nicotine Enhances Colon Cancer Cell Migration by Induction of Fibronectin
Authors: Po-Li Wei, MD
Li-Jen Kuo, MD
Ming-Te Huang, MD
Wen-Chien Ting, MD
Yuan-Soon Ho, PhD
Weu Wang, MD
Jane An, MD
Yu-Jia Chang, PhD
Publication date: 01-06-2011
Publisher: Springer-Verlag
Published in: Annals of Surgical Oncology / Issue 6/2011
Print ISSN: 1068-9265
Electronic ISSN: 1534-4681
DOI: https://doi.org/10.1245/s10434-010-1504-3

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Nicotine Enhances Colon Cancer Cell Migration by Induction of Fibronectin

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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