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01-10-2008 | Bone and Soft Tissue Sarcomas

A Large-Scale Study of MT1–MMP as a Marker for Isolated Tumor Cells in Peripheral Blood and Bone Marrow in Gastric Cancer Cases

Authors: Koshi Mimori, MD, Takeo Fukagawa, MD, Yoshimasa Kosaka, MD, Kenji Ishikawa, MD, Masaaki Iwatsuki, MD, Takehiko Yokobori, MD, Shigeo Hirasaki, MD, Yasushi Takatsuno, MD, Hiroyuki Sakashita, MD, Hideshi Ishii, MD, Mitsuru Sasako, MD, Masaki Mori, MD, FACS

Published in: Annals of Surgical Oncology | Issue 10/2008

Abstract

Background

Recently, a marker for predicting metastasis or recurrence precisely in solid cancers has been focused on instead of the identification of isolated tumor cells detected by epithelial genes in circulating system. We identified a candidate marker in gastric cancer by microarray and validated it through a quantitative real-time reverse transcriptase-polymerase chain reaction (RT-PCR) assay.

Methods

To identify metastasis-related genes, we performed cDNA microarray analysis of total RNA from whole bone marrow blood from six cases with metastasis and three cases without metastasis. We determined clinical significance of the identified gene by microarray analysis with quantitative real-time RT-PCR in bone marrow and peripheral blood from 810 cases of gastric cancer.

Results

We focused on membrane type 1 matrix metalloproteinase (MT1-MMP) as a candidate marker to predict distant metastasis among identified genes. MT1-MMP-positive expression in peripheral blood was associated with incidence of peritoneal dissemination, lymphatic permeation, vascular permeation, and lymph node metastasis. MT1-MMP-positive expression in bone marrow was also significantly related to the incidence of distant metastasis and peritoneal dissemination.

Conclusion

The expression of MT1-MMP in peripheral blood from gastric cancer cases was a powerful indicator of distant metastasis especially for peritoneal dissemination. The presence of MT1-MMP-expressing cells in bone marrow indicated higher risk for distant metastasis in gastric cancer cases.

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Title: A Large-Scale Study of MT1–MMP as a Marker for Isolated Tumor Cells in Peripheral Blood and Bone Marrow in Gastric Cancer Cases
Authors: Koshi Mimori, MD
Takeo Fukagawa, MD
Yoshimasa Kosaka, MD
Kenji Ishikawa, MD
Masaaki Iwatsuki, MD
Takehiko Yokobori, MD
Shigeo Hirasaki, MD
Yasushi Takatsuno, MD
Hiroyuki Sakashita, MD
Hideshi Ishii, MD
Mitsuru Sasako, MD
Masaki Mori, MD, FACS
Publication date: 01-10-2008
Publisher: Springer-Verlag
Published in: Annals of Surgical Oncology / Issue 10/2008
Print ISSN: 1068-9265
Electronic ISSN: 1534-4681
DOI: https://doi.org/10.1245/s10434-008-9916-z

Keynote webinar | Spotlight on medication adherence

Springer Medicine

A Large-Scale Study of MT1–MMP as a Marker for Isolated Tumor Cells in Peripheral Blood and Bone Marrow in Gastric Cancer Cases

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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