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01-05-2007 | Gastrointestinal Oncology

Detection of Minimal Gastric Cancer Cells in Peritoneal Washings by Focused Microarray Analysis with Multiple Markers: Clinical Implications

Authors: Kazuhiko Mori, MD, Tomohiro Suzuki, PhD, Hiroshi Uozaki, MD, Hayao Nakanishi, MD, Tetsuya Ueda, MD, Yoshihiro Matsuno, MD, Yasuhiro Kodera, MD, Hiromi Sakamoto, PhD, Nobuko Yamamoto, PhD, Mitsuru Sasako, MD, Michio Kaminishi, MD, Hiroki Sasaki, PhD

Published in: Annals of Surgical Oncology | Issue 5/2007

Abstract

Background

Peritoneal cytology is an important prognostic factor of gastric cancer. However, peritoneal cytology requires great skill, which may explain its low prevalence. A reverse transcriptase–polymerase chain reaction–based assay with multiple marker genes or immunocytochemistry was assessed as an alternative method of gathering the same kind of data as cytology.

Methods

Peritoneal washings from 179 patients with gastric cancer were analyzed by multiplex reverse transcriptase–polymerase chain reaction with 10 marker genes and subsequent hybridization to a customized oligo-nucleotide array. Results with this assay were either validated as a prognostic factor or confirmed by demonstrating the presence of cancer cells by immunocytochemical cytology.

Results

Only 1 (2.2%) of 44 disease-free cases was shown to be positive by the microarray assay, whereas 13 (93%) of 14 conventional cytology–positive cases were found to be positive. This assay further detected approximately one-third of cytology-negative patients either with peritoneal recurrence (7 of 20, 35%) or with non-peritoneal recurrence (6 of 22, 27%). A high concordance between the microarray assay and immunocytochemical cytology with five antibodies against CK20, FABP1, MUC2, TFF1, and MASPIN was confirmed. The clinical outcome of the microarray assay–positive cases was poor, as was that of the cytology-positive cases.

Conclusions

Our assay, though time-consuming and requiring special equipment, demonstrated a specificity and sensitivity equal to or better than cytology in our institutes. The minimal free peritoneal cancer cells detected by the microarray assay may provide the same clinical information as larger amounts of cancer cells for patients with gastric cancer. An anti-MASPIN antibody may be helpful in peritoneal cytology of gastric cancer.

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Title: Detection of Minimal Gastric Cancer Cells in Peritoneal Washings by Focused Microarray Analysis with Multiple Markers: Clinical Implications
Authors: Kazuhiko Mori, MD
Tomohiro Suzuki, PhD
Hiroshi Uozaki, MD
Hayao Nakanishi, MD
Tetsuya Ueda, MD
Yoshihiro Matsuno, MD
Yasuhiro Kodera, MD
Hiromi Sakamoto, PhD
Nobuko Yamamoto, PhD
Mitsuru Sasako, MD
Michio Kaminishi, MD
Hiroki Sasaki, PhD
Publication date: 01-05-2007
Publisher: Springer-Verlag
Published in: Annals of Surgical Oncology / Issue 5/2007
Print ISSN: 1068-9265
Electronic ISSN: 1534-4681
DOI: https://doi.org/10.1245/s10434-006-9321-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Detection of Minimal Gastric Cancer Cells in Peritoneal Washings by Focused Microarray Analysis with Multiple Markers: Clinical Implications

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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