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Open Access 01-12-2017 | Research

Higher levels of myelin phospholipids in brains of neuronal α-Synuclein transgenic mice precede myelin loss

Authors: Jessica Grigoletto, Katharina Pukaß, Ayelet Gamliel, Dana Davidi, Rachel Katz-Brull, Christiane Richter-Landsberg, Ronit Sharon

Published in: Acta Neuropathologica Communications | Issue 1/2017

Abstract

α-Synuclein is a protein involved in the pathogenesis of synucleinopathies, including Parkinson’s disease (PD), dementia with Lewy bodies (DLB) and multiple system atrophy (MSA). We investigated the role of neuronal α-Syn in myelin composition and abnormalities. The phospholipid content of purified myelin was determined by ³¹P NMR in two mouse lines modeling PD, PrP-A53T α-Syn and Thy-1 wt-α-Syn. Significantly higher levels of phospholipids were detected in myelin purified from brains of these α-Syn transgenic mouse models than in control mice. Nevertheless, myelin ultrastructure appeared intact. To further investigate the effect of α-Syn on myelin abnormalities, we systematically analyzed the striatum, a brain region associated with neurodegeneration in PD. An age and disease-dependent loss of myelin basic protein (MBP) signal was detected by immunohistochemistry in striatal striosomes (patches). The age-dependent loss of MBP signal was associated with lower P25α levels in oligodendrocytes. In addition, we found that α-Syn inhibited oligodendrocyte maturation and the formation of membranous sheets in vitro. Based on these results we concluded that neuronal α-Syn is involved in the regulation and/or maintenance of myelin phospholipid. However, axonal hypomyelination in the PD models is evident only in progressive stages of the disease and associated with α-Syn toxicity.

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Title: Higher levels of myelin phospholipids in brains of neuronal α-Synuclein transgenic mice precede myelin loss
Authors: Jessica Grigoletto
Katharina Pukaß
Ayelet Gamliel
Dana Davidi
Rachel Katz-Brull
Christiane Richter-Landsberg
Ronit Sharon
Publication date: 01-12-2017
Publisher: BioMed Central
Published in: Acta Neuropathologica Communications / Issue 1/2017
Electronic ISSN: 2051-5960
DOI: https://doi.org/10.1186/s40478-017-0439-3

At a glance: The STEP trials

Springer Medicine

Higher levels of myelin phospholipids in brains of neuronal α-Synuclein transgenic mice precede myelin loss

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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