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Open Access 01-12-2016 | Research

Association of CYP1A1, GSTM1 and GSTT1 gene polymorphisms with risk of non-small cell lung cancer in Andhra Pradesh region of South India

Authors: Vidyullatha Peddireddy, Siva Prasad Badabagni, Sandhya Devi Gundimeda, Vasudha Mamidipudi, Pardhanandana Reddy Penagaluru, Hema Prasad Mundluru

Published in: European Journal of Medical Research | Issue 1/2016

Abstract

Background

Lung cancer is one of the most preventable causes of death globally both in developed and developing countries. Although it is well established that smokers develop lung cancer, there are some smokers who are free from the disease risk. The predisposition to lung cancer is attributed to genetic polymorphisms in xenobiotic metabolizing genes. Reports on assessment of xenobiotic metabolizing genes like Cytochrome P 450 1A1 (CYP1A1), Glutathione -S -transferase M1 (GSTM1) and T1 (GSTT1) polymorphisms from India are meagre, and reports from Andhra Pradesh are lacking.

Methods and results

Assessment of polymorphisms in CYP1A1, GSTM1 and GSTT1 in NSCLC patients and healthy individuals specific to population of Andhra Pradesh, a South Indian state was attempted by multiplex PCR and RFLP, and this is the first study which tried to correlate oxidative stress with the polymorphisms in xenobiotic metabolizing genes. Results showed that CYP1A1 m1 ‘CC’ genotype was significantly associated with lung cancer susceptibility with a 2.3-fold risk, CYP1A1 m2 ‘AG’ gene polymorphisms with 8.8-fold risk and GSTT1 (−/−) genotype demonstrated a twofold risk of disease susceptibility.

Conclusions

A combined role of genetic polymorphisms and smoking status can be attributed for the cause of lung cancer. Further, the association between oxidative stress and genetic polymorphisms showed a correlation between GSTT1 and super oxide dismutase activity; CYP1A1 m1, m2 and GSTT1 with glutathione peroxidase activity; CYP1A1 m1 and GSTM1 with melondialdehyde levels; and CYP1A1 m1 and GSTT1 with 8-oxo-7,8-dihydro-2′-deoxyguanosine. A higher risk of lung cancer seems to be associated with combined gene polymorphisms of phase I and phase II enzymes than that ascribed to single gene polymorphism.

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Mannervik B, Danielson UH. Glutathione transferases–structure and catalytic activity. CRC Crit Rev Biochem. 1988;23:283–337.CrossRefPubMed

Gonzalez FJ. Molecular genetics of the P-450 superfamily. Pharmacol Ther. 1990;45:1–38.CrossRefPubMed

Hayes JD, Pulford DJ. The glutathione S-transferase supergene family: regulation of GST and the contribution of the isoenzymes to cancer chemoprotection and drug resistance. Crit Rev Biochem Mol Biol. 1995;30:445–600.CrossRefPubMed

Guengerich FP, Thier R, Persmark M, Taylor JB, Pemble SE, et al. Conjugation of carcinogens by theta class glutathione s-transferases: mechanisms and relevance to variations in human risk. Pharmacogenetics. 1995;5:S103–7.CrossRefPubMed

Bethesda M. SEER Cancer Statistics Review. National Institutes of Health. 1973–1998.

Gupta D, Boffetta P, Gaborieau V, Jindal SK. Risk factors of lung cancer in Chandigarh, India. Indian J Med Res. 2001;113:142–50.PubMed

Raunio H, Husgafvel-Pursiainen K, Anttila S, Hietanen E, Hirvonen A, et al. Diagnosis of polymorphisms in carcinogen-activating and inactivating enzymes and cancer susceptibility–a review. Gene. 1995;159:113–21.CrossRefPubMed

Shimada T, Hayes CL, Yamazaki H, Amin S, Hecht SS, et al. Activation of chemically diverse procarcinogens by human cytochrome P-450 1B1. Cancer Res. 1996;56:2979–84.PubMed

Barouki R, Morel Y. Repression of cytochrome P450 1A1 gene expression by oxidative stress: mechanisms and biological implications. Biochem Pharmacol. 2001;61:511–6.CrossRefPubMed

10.

Taningher M, Malacarne D, Izzotti A, Ugolini D, Parodi S. Drug metabolism polymorphisms as modulators of cancer susceptibility. Mutat Res. 1999;436:227–61.CrossRefPubMed

11.

Seidegard J, Pero RW, Markowitz MM, Roush G, Miller DG, et al. Isoenzyme(s) of glutathione transferase (class Mu) as a marker for the susceptibility to lung cancer: a follow up study. Carcinogenesis. 1990;11:33–6.CrossRefPubMed

12.

Bell DA, Thompson CL, Taylor J, Miller CR, Perera F, et al. Genetic monitoring of human polymorphic cancer susceptibility genes by polymerase chain reaction: application to glutathione transferase mu. Environ Health Perspect. 1992;98:113–7.PubMedPubMedCentral

13.

Vineis P, d’Errico A, Malats N, Boffetta P. Overall evaluation and research perspectives. IARC Sci Publ. 1999;148:403–8.PubMed

14.

Habdous M, Siest G, Herbeth B, Vincent-Viry M, Visvikis S. Glutathione S-transferases genetic polymorphisms and human diseases: overview of epidemiological studies. Ann Biol Clin (Paris). 2004;62:15–24.

15.

Inoue K, Asao T, Shimada T. Ethnic-related differences in the frequency distribution of genetic polymorphisms in the CYP1A1 and CYP1B1 genes in Japanese and Caucasian populations. Xenobiotica. 2000;30:285–95.CrossRefPubMed

16.

Hung RJ, Boffetta P, Brockmoller J, Butkiewicz D, Cascorbi I, et al. CYP1A1 and GSTM1 genetic polymorphisms and lung cancer risk in Caucasian non-smokers: a pooled analysis. Carcinogenesis. 2003;24:875–82.CrossRefPubMed

17.

Song N, Tan W, Xing D, Lin D. CYP1A1 polymorphism and risk of lung cancer in relation to tobacco smoking: a case–control study in China. Carcinogenesis. 2002;22:11–6.CrossRef

18.

Garte S, Gaspari L, Alexandrie AK, Ambrosone C, Autrup H, et al. Metabolic gene polymorphism frequencies in control populations. Cancer Epidemiol Biomarkers Prev. 2001;10:1239–48.PubMed

19.

Hong YS, Chang JH, Kwon OJ, Ham YA, Choi JH. Polymorphism of the CYP1A1 and glutathione-S-transferase gene in Korean lung cancer patients. Exp Mol Med. 1998;30:192–8.CrossRefPubMed

20.

Sugimura H, Wakai K, Genka K, Nagura K, Igarashi H, et al. Association of Ile462Val (Exon 7) polymorphism of cytochrome P450 IA1 with lung cancer in the Asian population: further evidence from a case-control study in Okinawa. Cancer Epidemiol Biomarkers Prev. 1998;7:413–7.PubMed

21.

Shields PG, Caporaso NE, Falk RT, Sugimura H, Trivers GE, et al. Lung cancer, race, and a CYP1A1 genetic polymorphism. Cancer Epidemiol Biomarkers Prev. 1993;2:481–5.PubMed

22.

Bouchardy C, Wikman H, Benhamou S, Hirvonen A, Dayer P, et al. CYP1A1 genetic polymorphisms, tobacco smoking and lung cancer risk in a French Caucasian population. Biomarkers. 1997;2:131–4.CrossRefPubMed

23.

Benhamou S, Lee WJ, Alexandrie AK, Boffetta P, Bouchardy C, et al. Meta- and pooled analyses of the effects of glutathione S-transferase M1 polymorphisms and smoking on lung cancer risk. Carcinogenesis. 2002;23:1343–50.CrossRefPubMed

24.

Vineis P, Malats M, Lang M, d’Errico A, Caporaso N, et al. Metabolic polymorphisms and susceptibility to cancer. IARC Sci Publ. 1999;148:1–150.PubMed

25.

Quinones L, Lucas D, Godoy J, Caceres D, Berthou F, et al. CYP1A1, CYP2E1 and GSTM1 genetic polymorphisms. The effect of single and combined genotypes on lung cancer susceptibility in Chilean people. Cancer Lett. 2001;174:35–44.CrossRefPubMed

26.

Yang XR, Wacholder S, Xu Z, Dean M, Clark V, et al. CYP1A1 and GSTM1 polymorphisms in relation to lung cancer risk in Chinese women. Cancer Lett. 2004;214:197–204.CrossRefPubMed

27.

Sobti RC, Onsory K, Al-Badran AI, Kaur P, Watanabe M, et al. CYP17, SRD5A2, CYP1B1, and CYP2D6 gene polymorphisms with prostate cancer risk in North Indian population. DNA Cell Biol. 2006;25:287–94.CrossRefPubMed

28.

Shah PP, Singh AP, Singh M, Mathur N, Mishra BN, et al. Association of functionally important polymorphisms in cytochrome P4501B1 with lung cancer. Mutat Res. 2008;643:4–10.CrossRefPubMed

29.

Lakkakula S, Maram R, Munirajan AK, Pathapati RM, Visveswara SB, et al. Functional PstI/RsaI polymorphisms in the CYP2E1 gene among south Indian populations. Asian Pac J Cancer Prev. 2013;14:179–82.CrossRefPubMed

30.

Sreeja L, Syamala V, Hariharan S, Madhavan J, Devan SC, et al. Possible risk modification by CYP1A1, GSTM1 and GSTT1 gene polymorphisms in lung cancer susceptibility in a South Indian population. J Hum Genet. 2005;50:618–27.CrossRefPubMed

31.

Kyaing NN, Islam MA, Sinha DN, Rinchen S. Social, economic and legal dimensions of tobacco and its control in South-East Asia region. Indian J Public Health. 2011;55:161–8.CrossRefPubMed

32.

Peddireddy V, Siva Prasad B, Gundimeda SD, Penagaluru PR, Mundluru HP. Assessment of 8-oxo-7, 8-dihydro-2′-deoxyguanosine and malondialdehyde levels as oxidative stress markers and antioxidant status in non-small cell lung cancer. Biomarkers. 2012;17:261–8.CrossRefPubMed

33.

Greene FL. The American Joint Committee on Cancer: updating the strategies in cancer staging. Bull Am Coll Surg. 2002;87:13–5.PubMed

34.

Song N, Tan W, Xing D, Lin D. CYP 1A1 polymorphism and risk of lung cancer in relation to tobacco smoking: a case-control study in China. Carcinogenesis. 2001;22:11–6.CrossRefPubMed

35.

Teixeira JP, Gaspar J, Martinho G, Silva S, Rodrigues S, et al. Aromatic DNA adduct levels in coke oven workers: correlation with polymorphisms in genes GSTP1, GSTM1, GSTT1 and CYP1A1. Mutat Res. 2002;517:147–55.CrossRefPubMed

36.

Schneider J, Bernges U, Philipp M, Woitowitz HJ. GSTM1, GSTT1, and GSTP1 polymorphism and lung cancer risk in relation to tobacco smoking. Cancer Lett. 2004;208:65–74.CrossRefPubMed

37.

Alexandrie AK, Nyberg F, Warholm M, Rannug A. Influence of CYP1A1, GSTM1, GSTT1, and NQO1 genotypes and cumulative smoking dose on lung cancer risk in a Swedish population. Cancer Epidemiol Biomarkers Prev. 2004;13:908–14.PubMed

38.

Alexandrie AK, Rannug A, Juronen E, Tasa G, Warholm M. Detection and characterization of a novel functional polymorphism in the GSTT1 gene. Pharmacogenetics. 2002;12:613–9.CrossRefPubMed

39.

Xu X, Kelsey KT, Wiencke JK, Wain JC, Christiani DC. Cytochrome P450 CYP1A1 MspI polymorphism and lung cancer susceptibility. Cancer Epidemiol Biomarkers Prev. 1996;5:687–92.PubMed

40.

Quinones L, Berthou F, Varela N, Simon B, Gil L, et al. Ethnic susceptibility to lung cancer: differences in CYP2E1, CYP1A1 and GSTM1 genetic polymorphisms between French Caucasian and Chilean populations. Cancer Lett. 1999;141:167–71.CrossRefPubMed

41.

Taioli E, Gaspari L, Benhamou S, Boffetta P, Brockmoller J, et al. Polymorphisms in CYP1A1, GSTM1, GSTT1 and lung cancer below the age of 45 years. Int J Epidemiol. 2003;32:60–3.CrossRefPubMed

42.

Sobti RC, Sharma S, Joshi A, Jindal SK, Janmeja A. Genetic polymorphism of the CYP1A1, CYP2E1, GSTM1 and GSTT1 genes and lung cancer susceptibility in a north indian population. Mol Cell Biochem. 2004;266:1–9.CrossRefPubMed

43.

Sobti RC, Kaur P, Kaur S, Janmeja AK, Jindal SK, et al. Combined effect of GSTM1, GSTT1 and GSTP1 polymorphisms on histological subtypes of lung cancer. Biomarkers. 2008;13:282–95.CrossRefPubMed

44.

Okada T, Kawashima K, Fukushi S, Minakuchi T, Nishimura S. Association between a cytochrome P450 CYPIA1 genotype and incidence of lung cancer. Pharmacogenetics. 1994;4:333–40.CrossRefPubMed

45.

Sreelekha TT, Ramadas K, Pandey M, Thomas G, Nalinakumari KR, et al. Genetic polymorphism of CYP1A1, GSTM1 and GSTT1 genes in Indian oral cancer. Oral Oncol. 2001;37:593–8.CrossRefPubMed

46.

Kumar M, Agarwal SK, Goel SK. Lung cancer risk in north Indian population: role of genetic polymorphisms and smoking. Mol Cell Biochem. 2009;322:73–9.CrossRefPubMed

47.

Nelson HH, Wiencke JK, Christiani DC, Cheng TJ, Zuo ZF, et al. Ethnic differences in the prevalence of the homozygous deleted genotype of glutathione S-transferase theta. Carcinogenesis. 1995;16:1243–5.CrossRefPubMed

48.

Sorensen M, Autrup H, Tjonneland A, Overvad K, Raaschou-Nielsen O. Glutathione S-transferase T1 null-genotype is associated with an increased risk of lung cancer. Int J Cancer. 2004;110:219–24.CrossRefPubMed

49.

Lan Q, He X, Costa DJ, Tian L, Rothman N, et al. Indoor coal combustion emissions, GSTM1 and GSTT1 genotypes, and lung cancer risk: a case-control study in Xuan Wei, China. Cancer Epidemiol Biomarkers Prev. 2000;9:605–8.PubMed

50.

Ghosh P, Basu A, Mahata J, Basu S, Sengupta M, et al. Cytogenetic damage and genetic variants in the individuals susceptible to arsenic-induced cancer through drinking water. Int J Cancer. 2006;118:2470–8.CrossRefPubMed

51.

Vettriselvi V, Vijayalakshmi K, Paul SF. Genetic variation of GSTM1, GSTT1 and GSTP1 genes in a South Indian population. Asian Pac J Cancer Prev. 2006;7:325–8.

52.

Peddireddy V, Badabagni SP, Gundimeda SD, Sultana S, Kadali K, et al. Genetic instability in peripheral lymphocytes as biological marker for non-small cell lung cancer patients in the South Indian state of Andhra Pradesh. Int J Biol Markers. 2014;29:e345–53.CrossRefPubMed

53.

Nakachi K, Imai K, Hayashi S, Kawajiri K. Polymorphisms of the CYP1A1 and glutathione S-transferase genes associated with susceptibility to lung cancer in relation to cigarette dose in a Japanese population. Cancer Res. 1993;53:2994–9.PubMed

54.

Brockmoller J, Kerb R, Drakoulis N, Nitz M, Roots I. Genotype and phenotype of glutathione S-transferase class mu isoenzymes mu and psi in lung cancer patients and controls. Cancer Res. 1993;53:1004–11.PubMed

55.

Sobti RC, Sharma S, Joshi A, Jindal SK, Janmeja A. CYP1A1 and CYP2D6 polymorphism and risk of lung cancer in a North Indian population. Biomarkers. 2003;8:415–28.CrossRefPubMed

56.

Shi X, Zhou S, Wang Z, Zhou Z, Wang Z. CYP1A1 and GSTM1 polymorphisms and lung cancer risk in Chinese populations: a meta-analysis. Lung Cancer. 2008;59:155–63.CrossRefPubMed

57.

Dialyna IA, Miyakis S, Georgatou N, Spandidos DA. Genetic polymorphisms of CYP1A1, GSTM1 and GSTT1 genes and lung cancer risk. Oncol Rep. 2003;10:1829–35.PubMed

58.

Prasad SB, Vidyullatha P, Vani GT, Devi RP, Rani UP, et al. Association of gene polymorphism in detoxification enzymes and urinary 8-OHdG levels in traffic policemen exposed to vehicular exhaust. Inhal Toxicol. 2013;25:1–8.PubMed

59.

Chiou HY, Hsueh YM, Hsieh LL, Hsu LI, Hsu YH, et al. Arsenic methylation capacity, body retention, and null genotypes of glutathione S-transferase M1 and T1 among current arsenic-exposed residents in Taiwan. Mutat Res. 1997;386:197–207.CrossRefPubMed

60.

Matsui A, Ikeda T, Enomoto K, Hosoda K, Nakashima H, et al. Increased formation of oxidative DNA damage, 8-hydroxy-2′-deoxyguanosine, in human breast cancer tissue and its relationship to GSTP1 and COMT genotypes. Cancer Lett. 2000;151:87–95.CrossRefPubMed

61.

Bergamaschi E, De Palma G, Mozzoni P, Vanni S, Vettori MV, et al. Polymorphism of quinone-metabolizing enzymes and susceptibility to ozone-induced acute effects. Am J Respir Crit Care Med. 2001;163:1426–31.CrossRefPubMed

62.

Kim YD, Lee CH, Nan HM, Kang JW, Kim H. Effects of genetic polymorphisms in metabolic enzymes on the relationships between 8-hydroxydeoxyguanosine levels in human leukocytes and urinary 1-hydroxypyrene and 2-naphthol concentrations. J Occup Health. 2003;45:160–7.CrossRefPubMed

63.

Breton CV, Kile ML, Catalano PJ, Hoffman E, Quamruzzaman Q, et al. GSTM1 and APE1 genotypes affect arsenic-induced oxidative stress: a repeated measures study. Environ Health. 2007;6:39.CrossRefPubMedPubMedCentral

64.

Buthbumrung N, Mahidol C, Navasumrit P, Promvijit J, Hunsonti P, et al. Oxidative DNA damage and influence of genetic polymorphisms among urban and rural schoolchildren exposed to benzene. Chem Biol Interact. 2008;172:185–94.CrossRefPubMed

65.

Park EY, Hong YC, Lee KH, Im MW, Ha E, et al. Maternal exposure to environmental tobacco smoke, GSTM1/T1 polymorphisms and oxidative stress. Reprod Toxicol. 2008;26:197–202.CrossRefPubMed

66.

Loft S, Moller P. Oxidative DNA damage and human cancer: need for cohort studies. Antioxid Redox Signal. 2006;8:1021–31.CrossRefPubMed

67.

Wu LL, Chiou CC, Chang PY, Wu JT. Urinary 8-OHdG: a marker of oxidative stress to DNA and a risk factor for cancer, atherosclerosis and diabetics. Clin Chim Acta. 2004;339:1–9.CrossRefPubMed

68.

Kuo HW, Chou SY, Hu TW, Wu FY, Chen DJ. Urinary 8-hydroxy-2′-deoxyguanosine (8-OHdG) and genetic polymorphisms in breast cancer patients. Mutat Res. 2007;631:62–8.CrossRefPubMed

Title: Association of CYP1A1, GSTM1 and GSTT1 gene polymorphisms with risk of non-small cell lung cancer in Andhra Pradesh region of South India
Authors: Vidyullatha Peddireddy
Siva Prasad Badabagni
Sandhya Devi Gundimeda
Vasudha Mamidipudi
Pardhanandana Reddy Penagaluru
Hema Prasad Mundluru
Publication date: 01-12-2016
Publisher: BioMed Central
Published in: European Journal of Medical Research / Issue 1/2016
Electronic ISSN: 2047-783X
DOI: https://doi.org/10.1186/s40001-016-0209-x

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Association of CYP1A1, GSTM1 and GSTT1 gene polymorphisms with risk of non-small cell lung cancer in Andhra Pradesh region of South India

Abstract

Background

Methods and results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods and results

Conclusions

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