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Annals of Intensive Care

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Open Access 01-12-2018 | Research

Plasma microRNAs levels are different between pulmonary and extrapulmonary ARDS patients: a clinical observational study

Authors: Yi Zheng, Song-qiao Liu, Qin Sun, Jian-feng Xie, Jing-yuan Xu, Qing Li, Chun Pan, Ling Liu, Ying-zi Huang

Published in: Annals of Intensive Care | Issue 1/2018

Abstract

Background

Mesenchymal stem cells (MSC) obviously alleviate the damage of the structure and function of pulmonary vascular endothelial cells (VEC). The therapeutic effects of MSC are significantly different between pulmonary ARDS (ARDSp) and extrapulmonary ARDS (ARDSexp). MicroRNAs (miRNAs), as important media of MSC regulating VEC, are not studied between ARDSp and ARDSexp. We aimed to explore the plasma levels difference of miRNAs that regulate VEC function and are associated with MSC (MSC-VEC-miRNAs) between ARDSp and ARDSexp patients.

Methods

MSC-VEC-miRNAs were obtained through reviewing relevant literatures screened in PubMed database. We enrolled 57 ARDS patients within 24 h of admission to the ICU and then collected blood samples, extracted plasma supernatant. Patients’ clinical data were collected. Then, plasma expression of MSC-VEC-miRNAs was measured by real-time fluorescence quantitative PCR. Simultaneously, plasma endothelial injury markers VCAM-1, vWF and inflammatory factors TNF-α, IL-10 were detected by ELISA method.

Results

Fourteen miRNAs were picked out after screening. A total of 57 ARDS patients were included in this study, among which 43 cases pertained to ARDSp group and 14 cases pertained to ARDSexp group. Plasma miR-221 and miR-27b levels in ARDSexp group exhibited significantly lower than that in ARDSp group (miR-221, 0.22 [0.12–0.49] vs. 0.57 [0.22–1.57], P = 0.008, miR-27b, 0.34 [0.10–0.46] vs. 0.60 [0.20–1.46], P = 0.025). Plasma vWF concentration in ARDSexp group exhibited significantly lower than that in ARDSp group (0.77 [0.29–1.54] vs. 1.80 [0.95–3.51], P = 0.048). Significant positive correlation was found between miR-221 and vWF in plasma levels (r = 0.688, P = 0.022). Plasma miR-26a and miR-27a levels in non-survival group exhibited significantly lower than that in survival group (miR-26a, 0.17 [0.08–0.20] vs. 0.69 [0.24–2.33] P = 0.018, miR-27a, 0.23 [0.16–0.58] vs. 1.45 [0.38–3.63], P = 0.021) in ARDSp patients.

Conclusion

Plasma miR-221, miR-27b and vWF levels in ARDSexp group are significantly lower than that in ARDSp group. Plasma miR-26a and miR-27a levels in non-survival group are significantly lower than that in survival group in ARDSp patients.

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Title: Plasma microRNAs levels are different between pulmonary and extrapulmonary ARDS patients: a clinical observational study
Authors: Yi Zheng
Song-qiao Liu
Qin Sun
Jian-feng Xie
Jing-yuan Xu
Qing Li
Chun Pan
Ling Liu
Ying-zi Huang
Publication date: 01-12-2018
Publisher: Springer International Publishing
Published in: Annals of Intensive Care / Issue 1/2018
Electronic ISSN: 2110-5820
DOI: https://doi.org/10.1186/s13613-018-0370-1

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Plasma microRNAs levels are different between pulmonary and extrapulmonary ARDS patients: a clinical observational study

Abstract

Background

Methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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