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EJNMMI Research

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Open Access 01-12-2015 | Original research

Correlation between apoptosis and left ventricular remodeling in subacute phase of myocardial ischemia and reperfusion

Authors: Hiroshi Wakabayashi, Junichi Taki, Anri Inaki, Kazuhiro Shiba, Ichiro Matsunari, Seigo Kinuya

Published in: EJNMMI Research | Issue 1/2015

Abstract

Background

To investigate whether an apoptotic process demonstrated by ^99mTc-annexin-V (^99mTc-AV) uptake correlates with left ventricular remodeling (LVR) after myocardial infarction, we assessed ^99mTc-AV uptake in rat model of myocardial ischemia and reperfusion.

Methods

The left coronary artery (LCA) of 15 rats was occluded for 20 to 30 min, followed by reperfusion. After 2 weeks, ^99mTc-AV was injected, and then 1 h later, ²⁰¹Tl was injected after reocclusion of the LCA. Dual-tracer autoradiography was performed to assess ^99mTc-AV uptake and the area at risk (AAR) by ²⁰¹Tl defect. ^99mTc-AV uptake ratio was calculated by dividing the count density of the AAR by that of the normally perfused area. In short-axis LV slices, LV cavity dilation index (DI) was calculated by dividing the area of LV cavity by that of the whole LV area. LV wall-thinning ratio (WTR) was calculated by dividing the LV wall thickness in the AAR by that of the normally perfused area.

Results

Significant ^99mTc-AV uptake in the AAR was observed in 10 rats. DI was significantly higher in rats with positive ^99mTc-AV uptake than in rats without uptake. WTR was smaller in rats with positive ^99mTc-AV uptake than in rats without uptake.

Conclusions

The data suggest ^99mTc-AV uptake in injured myocardium might correlate with LVR at 2 weeks after myocardial ischemia and reperfusion.

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Title: Correlation between apoptosis and left ventricular remodeling in subacute phase of myocardial ischemia and reperfusion
Authors: Hiroshi Wakabayashi
Junichi Taki
Anri Inaki
Kazuhiro Shiba
Ichiro Matsunari
Seigo Kinuya
Publication date: 01-12-2015
Publisher: Springer Berlin Heidelberg
Published in: EJNMMI Research / Issue 1/2015
Electronic ISSN: 2191-219X
DOI: https://doi.org/10.1186/s13550-015-0152-9

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Correlation between apoptosis and left ventricular remodeling in subacute phase of myocardial ischemia and reperfusion

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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