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EJNMMI Research
Published in: EJNMMI Research 1/2015

Open Access 01-12-2015 | Preliminary research

SPECT imaging of glioma with radioiodinated CLINDE: evidence from a mouse GL26 glioma model

Authors: Stergios Tsartsalis, Noé Dumas, Benjamin B Tournier, Tien Pham, Marcelle Moulin-Sallanon, Marie-Claude Grégoire, Yves Charnay, Philippe Millet

Published in: EJNMMI Research | Issue 1/2015

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Abstract

Background

Recent research has demonstrated the potential of 18-kDa translocator protein (TSPO) to serve as a target for nuclear imaging of gliomas. The aim of this study was to evaluate SPECT imaging of GL26 mouse glioma using radioiodinated CLINDE, a TSPO-specific tracer.

Methods

GL26 cells, previously transfected with an enhanced green fluorescent protein (EGFP)-expressing lentivirus, were stereotactically implanted in the striatum of C57/Bl6 mice. At 4 weeks post-injection, dynamic SPECT scans with [123I]CLINDE were performed. A displacement study assessed specificity of tracer binding. SPECT images were compared to results of autoradiography, fluorescence microscopy, in situ nucleic acid hybridization, histology, and immunohistochemistry. Western blotting was performed to verify TSPO production by the tumor.

Results

Specific uptake of tracer by the tumor is observed with a high signal-to-noise ratio. Tracer uptake by the tumor is indeed 3.26 ± 0.32 times higher than that of the contralateral striatum, and 78% of the activity is displaceable by unlabeled CLINDE. Finally, TSPO is abundantly expressed by the GL26 cells.

Conclusions

The present study demonstrates the feasibility of [123I]CLINDE SPECT in translational studies and underlines its potential for clinical glioma SPECT imaging.
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Metadata
Title
SPECT imaging of glioma with radioiodinated CLINDE: evidence from a mouse GL26 glioma model
Authors
Stergios Tsartsalis
Noé Dumas
Benjamin B Tournier
Tien Pham
Marcelle Moulin-Sallanon
Marie-Claude Grégoire
Yves Charnay
Philippe Millet
Publication date
01-12-2015
Publisher
Springer Berlin Heidelberg
Published in
EJNMMI Research / Issue 1/2015
Electronic ISSN: 2191-219X
DOI
https://doi.org/10.1186/s13550-015-0092-4

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