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Open Access 01-12-2017 | Research

Naturally occurring anti-glycan antibodies binding to Globo H-expressing cells identify ovarian cancer patients

Authors: Tatiana Pochechueva, Shahidul Alam, Andreas Schötzau, Alexander Chinarev, Nicolai V. Bovin, Neville F. Hacker, Francis Jacob, Viola Heinzelmann-Schwarz

Published in: Journal of Ovarian Research | Issue 1/2017

Abstract

Background

Glycosphingolipids are important compounds of the plasma membrane of mammalian cells and a number of them have been associated with malignant transformation and progression, reinforcing tumour aggressiveness and metastasis. Here we investigated the levels of naturally occurring anti-glycan antibodies to Globo H in blood plasma obtained from high-grade serous ovarian cancer patients (SOC) and women without gynaecological malignancies (control) using suspension glycan array technology employing chemically synthesized glycans as antibody targets.

Results

We found that anti-human Globo H IgG antibodies were able to significantly discriminate SOC from controls (P < 0.05). A combination with the clinically used tumour marker CA125 increased the diagnostic performance (AUC 0.8711). We next compared suspension array with standard flow cytometry in plasma samples and found that the level of anti-Globo H antibodies highly correlated (r = 0.992). The incubation of plasma-derived anti-glycan antibodies with chemically synthesized (presented on fluorescence microspheres) and native Globo H (expressed on Globo H-positive cell lines) revealed strong reactivity of naturally occurring human anti-Globo H antibodies towards its antigen expressed on ovarian cancer cells.

Conclusions

Our data demonstrate that human plasma-derived antibodies to Globo H as well as the presence of the antigen might be considered as therapeutic option in ovarian cancer.

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Title: Naturally occurring anti-glycan antibodies binding to Globo H-expressing cells identify ovarian cancer patients
Authors: Tatiana Pochechueva
Shahidul Alam
Andreas Schötzau
Alexander Chinarev
Nicolai V. Bovin
Neville F. Hacker
Francis Jacob
Viola Heinzelmann-Schwarz
Publication date: 01-12-2017
Publisher: BioMed Central
Published in: Journal of Ovarian Research / Issue 1/2017
Electronic ISSN: 1757-2215
DOI: https://doi.org/10.1186/s13048-017-0305-8

ACC 2024 Congress

Springer Medicine

Naturally occurring anti-glycan antibodies binding to Globo H-expressing cells identify ovarian cancer patients

Abstract

Background

Results

Conclusions

ACC 2024 Congress

Springer Medicine

Abstract

Background

Results

Conclusions

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