Top

Published in:

Open Access 01-12-2016 | Research

GnRH agonist triggering affects the kinetics of embryo development: a comparative study

Authors: Ali Sami Gurbuz, Funda Gode, Mehmet Sukru Uzman, Betul Ince, Melek Kaya, Necati Ozcimen, Emel Ebru Ozcimen, Ali Acar

Published in: Journal of Ovarian Research | Issue 1/2016

Abstract

Background

To evaluate the effects of an ovulation triggering agent, human chorionic gonadotropin (hCG), versus a gonadotropin-releasing hormone agonist (GnRHa) on early embryo development in vitro using a time-lapse system.

Methods

Retrospective analysis of a prospectively collected database. A total of 739 embryos from 152 infertile couples undergoing intracytoplasmic sperm injection cycles.

Interventions : Embryo culture in a time-lapse incubator (EmbryoScope, Vitrolife, Göteborg, Sweden). Main Outcome Measures: Embryo morphokinetic parameters.

Results

In the 152 women, 252 embryos were derived from GnRHa-triggered cycles compared with 487 embryos derived from hCG-triggered cycles. Time-lapse analysis revealed that embryos from cycles triggered by a GnRHa cleaved faster than embryos derived from hCG-triggered cycles.

Conclusion

Triggering with a GnRHa in in vitro fertilization cycles affects embryo kinetics.

Baxter Bendus AE, Mayer JF, Shipley SK, Catherino WH. Interobserver and intraobserver variation in day 3 embryo grading. Fertil Steril. 2006;86:1608–15.CrossRefPubMed

Montag M, Toth B, Strowitzki T. New approaches to embryo selection. Reprod Biomed Online. 2013;27(5):539–46.CrossRefPubMed

Armstrong S, Arroll N, Cree LM, Jordan V, Farquhar C. Time-lapse systems for embryo incubation and assessment in assisted reproduction. Cochrane Database Syst Rev. 2015;27(2):CD011320.

Steptoe PC, Edwards RG. Birth after reimplantation of a human embryo. Lancet. 1978;2(8085):366.CrossRefPubMed

Al-Inany HG, Youssef MA, Aboulghar M, Broekmans F, Sterrenburg M, Smit J, Abou-Setta AM. Gonadotropin-releasing hormone antagonists for assisted reproductive technology. Cochrane Database Syst Rev. 2011;11(5):CD001750.

Kol S, Humaidan P, Itskovitz-Eldor J. GnRH agonist ovulation trigger and hCG-based, progesterone-free luteal support: a proof of concept study. Hum Reprod. 2011;26:2874–7.CrossRefPubMed

Youssef MAF, Abdelmoty HI, Ahmed MAS, Elmohamady M. GnRH agonist for final oocyte maturation in GnRH antagonist co-treated IVF/ICSI treatment cycles: Systematic review and meta-analysis. J Adv Res. 2015;6:341–9.CrossRefPubMedPubMedCentral

Humaiden P, Papanikolaou EG, Kyrou D, Alsbjerg B, Polyzos NP, Devroey P, Fatemi HM. The luteal phase after GnRH-agonist triggering of ovulation: present and future perspectives. Reprod Biomed Online. 2012;24:134–41.

Kol S, Humaiden P. LH (as HCG) and FSH surges for final oocyte maturation: sometimes it takes two to tango? Reprod Biomed Online. 2010;21:590–2.CrossRefPubMed

10.

Munoz M, Cruz M, Humaidan P, Garrido N, Perez-Cano I, Meseguer M. The type of GnRH analogue used during controlled ovarian stimulation influences early embryo developmental kinetics: a time lapse study. Eur J Obstet Gynecol Reprod Biol. 2013;168:167–72.CrossRefPubMed

11.

Meseguer M, Herrero J, Tejera A, Hilligsoe KM, Ramsing NB, Remohi J. The use of morphokinetics as a predictor of embryo implantation. Hum Reprod. 2011;26:2658–71.CrossRefPubMed

12.

Rubio C, Mercader A, Alama P, Lizan C, Rodrigo L, Labarta E, Melo M, Pellicer A, Remohi J. Prospective cohort study in high responder oocyte donors using two hormonal stimulation protocols: impact on embryo aneuploidy and development. Hum Reproduc. 2010;25:2290–7.

13.

Baart EB, Macklon NS, Fauser BJ. Ovarian stimulation and embryo quality. Reproduct Biomed Online. 2009;18 Suppl 2:45–50.CrossRef

14.

Humaidan P, Polyzos NP, Alsbjerg B, Erb K, Mikkelsen AL, Elbaek HO, Papanikolaou EG, Andersen CY. GnRHa trigger and individualized luteal phase hCG support according to ovarian response to stimulation: two prospective randomized controlled multi-centre studies in IVF patients. Hum Reprod. 2013;28(9):2511–21.

15.

Orvieto R. Triggering final follicular maturation-hCG, GnRH-agonist or both, when and to whom? J Ovarian Res. 2015;8:60.CrossRefPubMedPubMedCentral

16.

Lundin K, Bergh C, Hardarson T. Early embryo cleavage is a strong indicator of embryo quality in human IVF. Hum Reprod. 2001;16:2652–7.CrossRefPubMed

17.

Fenwick J, Platteau P, Murdoch AP, Herbert M. Time from insemination to first cleavage predicts developmental competence of human preimplantation embryosin vitro. Hum Reprod. 2002;17:407–12.CrossRefPubMed

18.

Lemmen JG, Agerholm I, Ziebe S. Kinetic markers of human embryo quality using time-lapse recordings of IVF/ICSI fertilized oocytes. Reprod Biomed Online. 2008;17:385–91.CrossRefPubMed

19.

Wong CC, Loewke KE, Bossert NL, Behr B, De Jonge CJ, Baer TM, Reijo Pera RA. Non invasive imaging of human embryos before embryonic genome activation predicts development to the blastocyst stage. Nat Biotechnol. 2010;28:1115–21.

20.

Van Montfoort AP, Dumuolin JC, Kester AD, Evers JL. Early cleavage is a valuable addition to existing embryo selection parameters: a study using single embryo transfers. Hum Reprod. 2004;19:2103–8.CrossRefPubMed

21.

Fauser BC, De Jong D, Olivennes F, Wramsby H, Tay C, Itskovitz Eldor J, et al. Endocrine profiles after triggering of final oocyte maturation with GnRH agonist after cotreatment with the GnRH antagonist ganirelix during ovarian hyperstimulation for in vitro fertilization. J Clin Endocrinol Metab. 2002;87:709–15.CrossRefPubMed

22.

Casarini L, Lispi M, Longobardi S, Milosa F, La Marca A, Tagliassacchi D, et al. LH and HCG action on the same receptor results in quantitatively and qualitatively different intracellular signaling. PLoS One. 2012;7:e46682.CrossRefPubMedPubMedCentral

23.

Yding Andersen C, Westergaard LG, Figenschau Y, Bertheussen K, Forsdahl F. Endocrine composition of follicular fluid comparing human chorionic gonadotrophin to a gonadotrophin-releasing hormone agonist for ovulation induction. Hum Reprod. 1993;8:840–3.PubMed

24.

Zelinski-Wooten MB, Hutchison JS, Hess DL, Wolf DP, Stouffer RL. Follicle stimulating hormone alone supports follicle growth and oocyte development in gonadotrophin-releasing hormone antagonist-treated monkey. Hum Reprod. 1995;10:1658–66.PubMed

25.

Yding Andersen C, Leonardsen L, Ulloa-Aguirre A, Barrios-De Tomasi J, Moore L, Byskov AG. FSH-induced resumption of meiosis in mouse oocytes: effect of different isoforms. Mol Hum Reprod. 1999;5:726–31.CrossRefPubMed

26.

Erb TM, Vitek W, Wakim ANG. Gonadotropin realesing hormone agonist or human chorionic gonadotropin for final oocyte maturation in an oocyte donor program. Fertil Steril. 2010;93:374–8.CrossRefPubMed

27.

Lin MH, Wu FS, Lee RK, Li SH, Lin SY, Hwu YM. Dual trigger with combination of gonadotropin-releasing hormone agonist and human chorionic gonadotropin significantly improves the live-birth rate for normal responders in GnRH antagonist cycles. Fertil Steril. 2013;100(5):1296–302.CrossRefPubMed

28.

Decleer W, Osmanagaoglu K, Seynhave B, Kolibianakis S, Tarlatzis B, Devroey P. Comparison of hCG triggering versus hCG in combination with a GnRH agonist: a prospective randomized controlled trial. Facts Views Vis Obygyn. 2014;6(4):203–9.

29.

Uygur D, Alkan RN, Batuoglu S. Recurrent empty follicle syndrome. J Assist Reprod Genet. 2003;20:390–2.CrossRefPubMedPubMedCentral

30.

Li Z, Wang Y, Ledger W, Edgar DH, Sullivan EA. Clinical outcomes following cryopreservation of blastocysts by vitrification or slow freezing: a population-based cohort study. Hum Reprod. 2014;29(12):2794–801.CrossRefPubMed

Title: GnRH agonist triggering affects the kinetics of embryo development: a comparative study
Authors: Ali Sami Gurbuz
Funda Gode
Mehmet Sukru Uzman
Betul Ince
Melek Kaya
Necati Ozcimen
Emel Ebru Ozcimen
Ali Acar
Publication date: 01-12-2016
Publisher: BioMed Central
Published in: Journal of Ovarian Research / Issue 1/2016
Electronic ISSN: 1757-2215
DOI: https://doi.org/10.1186/s13048-016-0229-8

At a glance: The STEP trials

Springer Medicine

GnRH agonist triggering affects the kinetics of embryo development: a comparative study

Abstract

Background

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

Please log in to get access to this content

Other articles of this Issue 1/2016

Salpingectomy before assisted reproductive technologies: a systematic literature review

Used protocols for isolation and propagation of ovarian stem cells, different cells with different traits

Sertoli - Leydig cell tumor with retiform areas and overgrowth of rhabdomyosarcomatous elements: case report and literature review

Analysis of MDR genes expression and cross-resistance in eight drug resistant ovarian cancer cell lines

Pseudocarcinomatous hyperplasia of the fallopian tube mimicking tubal cancer: a radiological and pathological diagnostic challenge

Evaluation of factors predicting diminished ovarian reserve before and after laparoscopic cystectomy for ovarian endometriomas: a prospective cohort study