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Open Access 01-12-2016 | Research

Supplementation of transport and freezing media with anti-apoptotic drugs improves ovarian cortex survival

Authors: Laurie Henry, Maïté Fransolet, Soraya Labied, Silvia Blacher, Marie-Caroline Masereel, Jean-Michel Foidart, Agnès Noel, Michelle Nisolle, Carine Munaut

Published in: Journal of Ovarian Research | Issue 1/2016

Abstract

Background

Ovarian tissue preservation is proposed to patients at risk of premature ovarian failure, but this procedure still needs to be optimized. To limit injury during ovarian tissue cryopreservation, anti-apoptotic drugs were added to the transport and freezing media of ovarian cortex tissue.

Methods

Sheep ovaries were transported, prepared and frozen in solutions containing vehicle or anti-apoptotic drugs (Z-VAD-FMK, a pan-caspase inhibitor, or sphingosine-1-phosphate (S1P), a bioactive lipid). After the tissue was thawed, the ovarian cortex was cultured for 2 or 6 days. Follicular quantification and morphological and proliferation analyses were performed on histological sections.

Results

After 2 days of culture, S1P improved the quality of primordial follicles; higher densities of morphologically normal and proliferative primordial follicles were found. Z-VAD-FMK displayed similar effects by preserving global primordial follicular density, but this effect was evident after 6 days of culture. This drug also improved cell proliferation after 2 and 6 days of culture.

Conclusions

Our results showed that the addition of S1P or Z-VAD-FMK to the transport and freezing media prior to ovarian tissue cryopreservation improves primordial follicular quality and therefore improves global tissue survival. This should ultimately lead to improved fertility restoration after auto-transplantation.

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Title: Supplementation of transport and freezing media with anti-apoptotic drugs improves ovarian cortex survival
Authors: Laurie Henry
Maïté Fransolet
Soraya Labied
Silvia Blacher
Marie-Caroline Masereel
Jean-Michel Foidart
Agnès Noel
Michelle Nisolle
Carine Munaut
Publication date: 01-12-2016
Publisher: BioMed Central
Published in: Journal of Ovarian Research / Issue 1/2016
Electronic ISSN: 1757-2215
DOI: https://doi.org/10.1186/s13048-016-0216-0

At a glance: The STEP trials

Springer Medicine

Supplementation of transport and freezing media with anti-apoptotic drugs improves ovarian cortex survival

Abstract

Background

Methods

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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