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Journal of Ovarian Research
Published in: Journal of Ovarian Research 1/2015

Open Access 01-12-2015 | Research

Clinical implication of the serum galectin-1 expression in epithelial ovarian cancer patients

Authors: Le Chen, Ying Yao, Lijuan Sun, Jiajia Zhou, Jingshi Liu, Jing Wang, Junjun Li, Jie Tang

Published in: Journal of Ovarian Research | Issue 1/2015

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Abstract

Background

galectin-1 has been implicated in tumor invasion and metastasis and is frequently over-expressed in epithelial ovarian cancer (EOC), but its potential as a biomarker remains unclear. In this novel study, we have explored the possible use of galectin-1 as a biomarker for EOC.

Methods

galectin-1 in sera was evaluated by ELISA in a pilot panel of EOC patients, healthy volunteers, patients with benign gynecologic tumors or other gynecologic malignancies. We examined galectin-1 expression in EOC tumor samples by Western Blot, qRT-PCR and immunohistochemistry. In vitro experiments were conducted to elucidate the biologic role of galectin-1 in EOC progression using over-expression of galectin-1 in OVCAR-3 cells. We also looked for the association of galectin-1 expression with clinic pathological variables and survival outcomes in EOC.

Results

A significant difference was detected in serum galectin-1 between EOC patients with non-metastatic and those with metastatic disease, but not between EOC patients and healthy volunteers. It increased in recurrent cases and decreased after debulking surgery. Both of galectin-1 mRNA and protein levels were increased in 90 % of the examined EOC tissue samples, compared with a wedge resection of a normal ovary. High galectin-1 in peritumor stroma was primarily detected in advanced stages of EOC. Over expression of galectin-1 significantly increased the ability of OVCAR-3 cells’ migration and invasion.

Conclusions

Our results suggest that galectin-1 might play a role in tumor progression and be associated with poor outcome in EOC. It could be a novel prognostic and progression biomarker in EOC patients.
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Metadata
Title
Clinical implication of the serum galectin-1 expression in epithelial ovarian cancer patients
Authors
Le Chen
Ying Yao
Lijuan Sun
Jiajia Zhou
Jingshi Liu
Jing Wang
Junjun Li
Jie Tang
Publication date
01-12-2015
Publisher
BioMed Central
Published in
Journal of Ovarian Research / Issue 1/2015
Electronic ISSN: 1757-2215
DOI
https://doi.org/10.1186/s13048-015-0206-7

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