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Open Access 01-12-2015 | Research

Retinoic acid has the potential to suppress endometriosis development

Authors: Yoshiaki Yamagata, Eiichi Takaki, Masahiro Shinagawa, Maki Okada, Kosuke Jozaki, Lifa Lee, Shun Sato, Ryo Maekawa, Toshiaki Taketani, Hiromi Asada, Hiroshi Tamura, Akira Nakai, Norihiro Sugino

Published in: Journal of Ovarian Research | Issue 1/2015

Abstract

Background

Despite endometriosis is common estrogen dependent disease afflicting women in reproductive age, the pathogenesis has not been fully elucidated. Retinoic acid has various functions in cells as biologic modulator, and aberrant retinoid metabolism seems to be involved in the lesions of endometriosis. In order to evaluate the potential of all-trans retinoic acid (ATRA) for therapeutic treatment, a transcriptome analysis and estradiol measurements in cultured endometriotic cells and tissues were conducted.

Methods

The mRNA expression levels in ATRA-treated endometriotic stromal cells (ESC) isolated from ovarian endometrial cysts (OEC) were investigated. Estradiol production in OEC tissues was also investigated.

Results

In the isolated ESC culture supplemented with ATRA for four days, total RNA was extracted followed by a transcriptome analysis using GeneChip. Forty-nine genes were upregulated and four genes were down-regulated by the ATRA treatment. Many upregulated genes were associated with the negative regulation of cellular proliferation. In addition, ATRA treatment decreased the mRNA expression of 17-beta-dehydrogenase 2 (HSD17B2) which converts estradiol into estrone in a dose-dependent manner, and the ELISA measurements indicated that estradiol production in the OEC tissue was inhibited by ATRA treatment.

Conclusions

Retinoic acid has the potential to suppress endometriosis development.

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Title: Retinoic acid has the potential to suppress endometriosis development
Authors: Yoshiaki Yamagata
Eiichi Takaki
Masahiro Shinagawa
Maki Okada
Kosuke Jozaki
Lifa Lee
Shun Sato
Ryo Maekawa
Toshiaki Taketani
Hiromi Asada
Hiroshi Tamura
Akira Nakai
Norihiro Sugino
Publication date: 01-12-2015
Publisher: BioMed Central
Published in: Journal of Ovarian Research / Issue 1/2015
Electronic ISSN: 1757-2215
DOI: https://doi.org/10.1186/s13048-015-0179-6

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Retinoic acid has the potential to suppress endometriosis development

Abstract

Background

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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