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Journal of Ovarian Research

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Open Access 01-12-2014 | Research

In vivo fate mapping of cryopreserved murine ovarian grafts

Authors: Chi-Huang Chen, Shun-Jen Tan, Chii-Ruey Tzeng

Published in: Journal of Ovarian Research | Issue 1/2014

Abstract

Background

Cryopreservation of ovarian tissue has been suggested as an alternative to restore fertility for ovarian failure before chemotherapy.

Methods

Ovaries of donor FVB/N-Tg (Pol II–Luc) Ltc transgenic mice (n = 5) were cryopreserved and transplanted to the back muscles of recipient FVB/NJNarl wild-type mice that had undergone bilateral oophorectomy. We evaluated the fate of cryopreserved murine ovarian grafts by in vivo bioluminescent imaging (BLI), AMH mRNA expression and follicle counts.

Results

There were significantly stronger BLI signals in the fresh ovaries than in the frozen–thawed ones. The number of primordial follicles was significantly lower in frozen–thawed ovaries at 10 days after transplantation (P < 0.001). The AMH mRNA expression was significantly lower in the frozen–thawed ovaries (P < 0.001), showing that unavoidable harm occurs after transplantation.

Conclusions

Ovarian cryopreservation by slow freezing compromises ovarian reserve by cryoinjury and ischemia, evident at an early stage after transplantation.

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Title: In vivo fate mapping of cryopreserved murine ovarian grafts
Authors: Chi-Huang Chen
Shun-Jen Tan
Chii-Ruey Tzeng
Publication date: 01-12-2014
Publisher: BioMed Central
Published in: Journal of Ovarian Research / Issue 1/2014
Electronic ISSN: 1757-2215
DOI: https://doi.org/10.1186/s13048-014-0081-7

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

In vivo fate mapping of cryopreserved murine ovarian grafts

Abstract

Background

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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