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Chinese Medicine
Published in: Chinese Medicine 1/2018

Open Access 01-12-2018 | Research

Rapid screening and identification of bioactive compounds specifically binding to beta 2-adrenoceptor from San-ao decoction using affinity magnetic fine particles coupled with high-performance liquid chromatography–mass spectrometry

Authors: Fuhuan Fei, Huanmei Sun, Xixi Cheng, Jiajun Liu, Jing Wang, Qian Li, Yajun Zhang

Published in: Chinese Medicine | Issue 1/2018

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Abstract

Background

San-ao decoction (SAD) has been widely used in Chinese medicine against respiratory diseases, such as asthma and rhinallergosis. The bioactive compounds for such pharmacological action remain unknown.

Methods

We developed a methodology to isolate the bioactive compounds of SAD. The assay involved the immobilization of beta 2-adrenoceptor (β2-AR) onto magnetic fine particles, the capture of target compounds by the immobilized receptor, the identification of the receptor bound compounds by reversed-phase high-performance liquid chromatography coupled with tandem mass spectrometry.

Results

Vicenin, shaftoside, isoshaftoside, liquiritin apioside and isoliquiritin apioside were identified as β2-AR ligands in SAD extract. The binding of these compounds to β2-AR occurred on serine169, serine170 and phenylalanine256 of the receptor.

Conclusions

The developed methodology has high stability and specificity for recognizing and isolating target compounds. It is an alternative method for rapidly screening bioactive compounds of immobilized receptor from Chinese prescriptions.
Appendix
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Metadata
Title
Rapid screening and identification of bioactive compounds specifically binding to beta 2-adrenoceptor from San-ao decoction using affinity magnetic fine particles coupled with high-performance liquid chromatography–mass spectrometry
Authors
Fuhuan Fei
Huanmei Sun
Xixi Cheng
Jiajun Liu
Jing Wang
Qian Li
Yajun Zhang
Publication date
01-12-2018
Publisher
BioMed Central
Published in
Chinese Medicine / Issue 1/2018
Electronic ISSN: 1749-8546
DOI
https://doi.org/10.1186/s13020-018-0207-8

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