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Chinese Medicine
Published in: Chinese Medicine 1/2017

Open Access 01-12-2017 | Research

Danggui Buxue Tang, a simple Chinese formula containing Astragali Radix and Angelicae Sinensis Radix, stimulates the expressions of neurotrophic factors in cultured SH-SY5Y cells

Authors: Amy G. W. Gong, Huai Y. Wang, Tina T. X. Dong, Karl W. K. Tsim, Y. Z. Zheng

Published in: Chinese Medicine | Issue 1/2017

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Abstract

Background

Danggui Buxue Tang (DBT), a phytoestrogen-enriched Chinese herbal formula, serves as dietary supplement in stimulating the “Blood” functions of menopausal women. In traditional Chinese medicine (TCM) theory, “Blood” has a strong relationship with brain activities. Previous studies supported that some ingredients of DBT possessed neuronal beneficial functions. Therefore, the neurotrophic function and the mechanistic action of DBT were systematically evaluated in cultured human neuroblastoma SH-SY5Y cells.

Methods

The DBT-triggered protein expressions were analyzed by western blotting, while the transcriptional activities of promoters coding for related genes were revealed by luciferase assays. For mechanistic analysis of DBT, Erk1/2 and its inhibitor U0126 were analyzed.

Results

The application of DBT in cultured neuroblastoma cells showed the efficacies in: (1) up-regulation of nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF) and glial cell line-derived neurotrophic factor (GDNF); (2) activation of transcriptional activities of promoters coding for NGF, BDNF, GDNF; (3) activation of Erk1/2 and CREB; and (4) attenuation of the neurotrophic factor expression by the treatment of an Erk1/2 inhibitor.

Conclusions

Our study supports that MAPK/Erk pathway acts as fundamental role in monitoring DBT-induced expression of neurotrophic factors in cultured human neuroblastoma cell. These results shed light in developing the working mechanism of this ancient herbal decoction for its neuronal function.
Appendix
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Literature
1.
Dong TTX, Zhao KJ, Gao QT, Ji ZN, Zhu TT, Li J, et al. Chemical and biological assessment of a Chinese herbal decoction containing Radix. Astragali and Radix Angelicae Sinensis: determination of drug ratio in having optimized properties. J Agric Food Chem. 2006;54:2767–74.CrossRefPubMed
2.
Gong AGW, Li N, Lau KM, Lee PSC, Yan L, Xu ML, et al. Calycosin orchestrates the functions of Danggui Buxue Tang, a Chinese herbal decoction composing of Astragali Radix and Angelica Sinensis Radix: an evaluation by using calycosin-knock out herbal extract. J Ethnopharmacol. 2015;16:150–7.CrossRef
3.
Gao QT, Choi RC, Cheung AW, Zhu JT, Li J, Chu GK, et al. Danggui Buxue Tang—a Chinese herbal decoction activates the phosphorylations of extracellular signal-regulated kinase and estrogen receptor alpha in cultured MCF-7 cells. FEBS Lett. 2007;581:233–40.CrossRefPubMed
4.
Lin HQ, Gong AG, Wang HY, Duan R, Dong TT, Zhao KJ, et al. Danggui Buxue Tang (Astragali Radix and Angelicae Sinensis Radix) for menopausal symptoms: a review. J Ethnopharmacol. 2017;199:205–10.CrossRefPubMed
5.
Ning L, Chen CX, Jin RM, Wu YP, Zhang HG, Sun CL, et al. Effect of components of dang-gui- bu-xue decoction on hematopenia. Zhongguo Zhong Yao Za Zhi. 2002;27:50–3.PubMed
6.
Zierau O, Zheng KY, Papke A, Dong TT, Tsim KW, Vollmer G. Functions of Danggui Buxue Tang, a Chinese herbal decoction containing Astragali Radix and Angelicae Sinensis Radix, in uterus and liver are both estrogen receptor-dependent and -independent. Evid Based Complement Alternat Med. 2014. doi:10.​1155/​2014/​438531.
7.
Wang WL, Sheu SY, Chen YS, Kao ST, Fu YT, Kuo TF, et al. Enhanced bone tissue regeneration by porous gelatin composites loaded with the Chinese herbal decoction Danggui Buxue Tang. PLoS ONE. 2015;10:e0131999.CrossRefPubMedPubMedCentral
8.
Yang X, Huang CG, Du SY, Yang SP, Zhang X, Liu JY, et al. Effect of Danggui Buxue Tang on immune-mediated aplastic anemia bone marrow proliferation mice. Phytomedicine. 2014;21:640–6.CrossRefPubMed
9.
Lv J, Zhao Z, Chen Y, Wang Q, Tao Y, Yang L, et al. The Chinese herbal decoction Danggui Buxue Tang inhibits angiogenesis in a rat model of liver fibrosis. Evid Based Complement Alternat Med. 2012. doi:10.​1155/​2012/​284963.
10.
Zheng KYZ, Choi RC, Guo AJ, Bi CW, Zhu KY, Du CY, et al. The membrane permeability of Astragali Radix-derived formononetin and calycosin is increased by Angelicae Sinensis Radix in Caco-2 cells: a synergistic action of an ancient herbal decoction Danggui Buxue Tang. J Pharm Biomed Anal. 2012;70:671–9.CrossRefPubMed
11.
Zhang L, Gong AG, Riaz K, Ho CM, Lin HQ, Dong TT, et al. A novel combination of four flavonoids derived from Astragali Radix relieves the symptoms of cyclophosphamide-induced anemic rats. FEBS Open Bio. 2017;7:318–23.CrossRefPubMedPubMedCentral
12.
Xu SL, Zhu KY, Bi CW, Yan L, Men SW, Dong TT, et al. Flavonoids, derived from traditional Chinese medicines, show roles in the differentiation of neurons: possible targets in developing health food products. Birth Defects Res C Embryo Today. 2013;99:292–9.CrossRefPubMed
13.
Espinet C, Gonzalo H, Fleitas C, Menal MJ, Egea J. Oxidative stress and neurodegenerative diseases: a neurotrophic approach. Curr Drug Targets. 2015;16:20–30.CrossRefPubMed
14.
Murray B, Alessandrini A, Cole AJ, Yee AG, Furshpan EJ. Inhibition of the p44/42 MAP kinase pathway protects hippocampal neurons in a cell-culture model of seizure activity. Proc Natl Acad Sci. 1998;95:11975–80.CrossRefPubMedPubMedCentral
15.
Satoh T, Nakatsuka D, Watanabe Y, Nagata I, Kikuchi H, Namura S. Neuroprotection by MAPK/ERK kinase inhibition with U0126 against oxidative stress in a mouse neuronal cell line and rat primary cultured cortical neurons. Neurosci Lett. 2000;288:163–6.CrossRefPubMed
16.
Gong AG, Lau KM, Xu ML, Lin HQ, Dong TT, Zheng KY, et al. The estrogenic properties of Danggui Buxue Tang, a Chinese herbal decoction, are triggered predominantly by calycosin in MCF-7 cells. J Ethnopharmacol. 2016;189:81–9.CrossRefPubMed
17.
Yang Q, Feng B, Zhang K, Guo YY, Liu SB, Wu YM, et al. Excessive astrocyte-derived neurotrophin-3 contributes to the abnormal neuronal dendritic development in a mouse model of fragile X syndrome. PLoS Genet. 2012;8:e1003172.CrossRefPubMedPubMedCentral
18.
Yan L, Xu SL, Zhu KY, Lam KY, Xin G, Maiwulanjiang M, et al. Optimizing the compatibility of paired-herb in an ancient Chinese herbal decoction Kai-Xin-San in activating neurofilament expression in cultured PC12 cells. J Ethnopharmacol. 2015;162:155–62.CrossRefPubMed
19.
Pan SY, Chen SB, Dong HG, Yu ZL, Dong JC, Long ZX, et al. New perspectives on Chinese herbal medicine (Zhong-Yao) research and development. Evid Based Complement Alternat Med. 2011. doi:10.​1093/​ecam/​neq056.
20.
Tohda C, Tamura T, Matsuyama S, Komatsu K. Promotion of axonal maturation and prevention of memory loss in mice by extracts of Astragalus mongholicus. Br J Pharmacol. 2006;149:532–41.CrossRefPubMedPubMedCentral
21.
Sajad M, Zargan J, Zargar MA, Sharma J, Umar S, Arora R, et al. Quercetin prevents protein nitration and glycolytic block of proliferation in hydrogen peroxide insulted cultured neuronal precursor cells (NPCs): implications on CNS regeneration. Neurotoxicology. 2013;36:24–33.CrossRefPubMed
22.
Oh SB, Park HR, Jang YJ, Choi SY, Son TG, Lee J. Baicalein attenuates impaired hippocampal neurogenesis and the neurocognitive deficits induced by ã-ray radiation. Br J Pharmacol. 2013;168:421–31.CrossRefPubMed
23.
Youdim KA, Dobbie MS, Kuhnle G, Proteggente AR, Abbott NJ, Rice-Evans C. Interaction between flavonoids and the blood–brain barrier: in vitro studies. J Neurochem. 2003;85:180–92.CrossRefPubMed
24.
Guo X, Chen ZH, Wang HL, Liu ZC, Wang XP, Zhou BH, et al. WSKY, a traditional Chinese decoction, rescues cognitive impairment associated with NMDA receptor antagonism by enhancing BDNF/ERK/CREB signaling. Mol Med Rep. 2015;11:2927–34.PubMed
25.
An L, Zhang YZ, Yu NJ, Liu XM, Zhao N, Yuan L, et al. The total flavonoids extracted from Xiaobuxin-Tang up-regulate the decreased hippocampal neurogenesis and neurotrophic molecules expression in chronically stressed rats. Prog Neuropsychopharmacol Biol Psychiatry. 2008;32:1484–90.CrossRefPubMed
26.
Xing H, Zhang K, Zhang R, Shi H, Bi K, Chen X. Anti-depressant-like effect of the water extract of the fixed combination of Gardenia jasminoides, Citrus aurantium and Magnolia officinalis in a rat model of chronic unpredictable mild stress. Phytomedicine. 2015;22:1178–85.CrossRefPubMed
27.
Budni J, Bellettini-Santos T, Mina F, Garcez ML, Zugno AI. The involvement of BDNF, NGF and GDNF in aging and Alzheimer’s disease. Aging Dis. 2015;6:331–41.CrossRefPubMedPubMedCentral
28.
Farrand AQ, Gregory RA, Scofield MD, Helke KL, Boger HA. Effects of aging on glutamate neurotransmission in the substantia nigra of GDNF heterozygous mice. Neurobiol Aging. 2015;36:1569–76.CrossRefPubMed
29.
Ferreira D, Westman E, Eyjolfsdottir H, Almqvist P, Lind G, Linderoth B, et al. Brain changes in Alzheimer’s disease patients with implanted encapsulated cells releasing nerve growth factor. J. Alzheimers Dis. 2015;43:1059–72.PubMed
30.
Kim EK, Choi EJ. Pathological roles of MAPK signaling pathways in human diseases. Biochim Biophys Acta. 2010;1802:396–405.CrossRefPubMed
31.
Villalba M, Bockaert J, Journot L. Pituitary adenylate cyclase-activating polypeptide (PACAP-38) protects cerebellar granule neurons from apoptosis by activating the mitogen-activated protein kinase (MAP kinase) pathway. J Neurosci. 1997;17:83–90.PubMed
32.
Cao R, Lee B, Cho HY, Saklayen S, Obrietan K. Photic regulation of the mTOR signaling pathway in the suprachiasmatic circadian clock. Mol Cell Neurosci. 2008;38:312–24.CrossRefPubMedPubMedCentral
Metadata
Title
Danggui Buxue Tang, a simple Chinese formula containing Astragali Radix and Angelicae Sinensis Radix, stimulates the expressions of neurotrophic factors in cultured SH-SY5Y cells
Authors
Amy G. W. Gong
Huai Y. Wang
Tina T. X. Dong
Karl W. K. Tsim
Y. Z. Zheng
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Chinese Medicine / Issue 1/2017
Electronic ISSN: 1749-8546
DOI
https://doi.org/10.1186/s13020-017-0144-y

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