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Journal of Orthopaedic Surgery and Research
Published in: Journal of Orthopaedic Surgery and Research 1/2019

Open Access 01-12-2019 | Research article

Design and evaluation of nano-hydroxyapatite/poly(vinyl alcohol) hydrogels coated with poly(lactic-co-glycolic acid)/nano-hydroxyapatite/poly(vinyl alcohol) scaffolds for cartilage repair

Authors: Weiping Su, Yihe Hu, Min Zeng, Mingqing Li, Shaoru Lin, Yangying Zhou, Jie Xie

Published in: Journal of Orthopaedic Surgery and Research | Issue 1/2019

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Abstract

Background

Poly(vinyl alcohol) (PVA) hydrogels have been widely used in synthetic cartilage materials. However, limitations of PVA hydrogels such as poor biomechanics and limited cell ingrowth remain challenges in this field.

Methods

This work aimed to design novel nano-hydroxyapatite (nano-HA)/poly(vinyl alcohol) (PVA) hydrogels coated with a poly(lactic-co-glycolic acid) (PLGA)/nano-HA/PVA scaffold to counter the limitations of PVA hydrogels. The core, comprising nano-HA/PVA hydrogel, had the primary role of bearing the mechanical load. The peripheral structure, composed of PLGA/nano-HA/PVA, was designed to favor interaction with surrounding cartilage.

Results

The double-layer HA/PVA hydrogel coated with PLGA/HA/PVA scaffold was successfully prepared using a two-step molding method, and the mechanical properties and biocompatibility were characterized. The mechanical properties of the novel PLGA/HA/PVA scaffold modified HA/PVA hydrogel were similar to those of native cartilage and showed greater sensitivity to compressive stress than to tensile stress. Rabbit chondrocytes were seeded in the composites to assess the biocompatibility and practicability in vitro. The results showed that the peripheral component comprising 30 wt% PLGA/5 wt% HA/15 wt% PVA was most conducive to rabbit chondrocyte adhesion and proliferation.

Conclusions

The study indicated that the double-layer HA/PVA hydrogel coated with PLGA/HA/PVA scaffold has the potential for cartilage repair.
Appendix
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Literature
1.
Bauer KL. Osteochondral injuries of the knee in pediatric patients [J]. J Knee Surg. 2018;31(5):382–91.CrossRef
2.
Bhattacharjee M, Coburn J, Centola M, et al. Tissue engineering strategies to study cartilage development, degeneration and regeneration [J]. Adv Drug Deliv Rev. 2015;84:107–22.CrossRef
3.
Makris EA, Gomoll AH, Malizos KN, et al. Repair and tissue engineering techniques for articular cartilage [J]. Nat Rev Rheumatol. 2015;11(1):21–34.CrossRef
4.
McNickle AG, Provencher MT, Cole BJ. Overview of existing cartilage repair technology. Sports Med Arthrosc Rev. 2008;16(4):196–201.CrossRef
5.
Thiede RM, Lu Y, Markel MD. A review of the treatment methods for cartilage defects. Vet Comp Orthop Traumatol. 2012;25(4):263–72.CrossRef
6.
Hurtig M, Pearce S, Warren S, Kalra M, Miniaci A. Arthroscopic mosaic arthroplasty in the equine third carpal bone. Vet Surg. 2001;30(3):228–39.CrossRef
7.
Pan Y, Xiong D. Study on compressive mechanical properties of nanohydroxyapatite reinforced poly (vinyl alcohol) gel composites as biomaterial. J Mater Sci Mater Med. 2009;20(6):1291–7.CrossRef
8.
Pan Y, Xiong D, Gao F. Viscoelastic behavior of nano-hydroxyapatite reinforced poly (vinyl alcohol) gel biocomposites as an articular cartilage. J Mater Sci Mater Med. 2008;19(5):1963–9.CrossRef
9.
Naahidi S, Jafari M, Logan M, et al. Biocompatibility of hydrogel-based scaffolds for tissue engineering applications. Biotechnol Adv. 2017;35(5):530–44.CrossRef
10.
Baykal D, Underwood RJ, Mansmann K, et al. Evaluation of friction properties of hydrogels based on a biphasic cartilage model [J]. J Mech Behav Biomed Mater. 2013;28:263–73.CrossRef
11.
Khandan A, Jazayeri H, Fahmy MD, Razavi M. Hydrogels: types, structure, properties, and applications [J]. Biomat Tiss Eng. 2017;4(27):143–69.
12.
Baker MI, Walsh SP, Schwartz Z, et al. A review of polyvinyl alcohol and its uses in cartilage and orthopedic applications [J]. J Biomed Mater Res B Appl Biomater. 2012;100(5):1451–7.CrossRef
13.
Kobayashi M, Chang YS, Oka M. A two year in vivo study of polyvinyl alcohol-hydrogel (PVA-H) artificial meniscus [J]. Biomaterials. 2005;26(16):3243–8.CrossRef
14.
Heydary HA, Karamian E, Poorazizi E, Khandan A, Heydaripour J. A novel nano-fiber of Iranian gum tragacanth-polyvinyl alcohol/nanoclay composite for wound healing applications. Procedia Materials Science. 2015;11:176–182.CrossRef
15.
Maher SA, Doty SB, Torzilli PA, et al. Nondegradable hydrogels for the treatment of focal cartilage defects. J Biomed Mater Res A. 2007;83(1):145–55.CrossRef
16.
Maiolo AS, Amado MN, Gonzalez JS, Alvarez VA. Development and characterization of poly (vinyl alcohol) based hydrogels for potential use as an articular cartilage replacement. Mater Sci Eng C Mater Biol Appl. 2012;32(6):1490–5.CrossRef
17.
Pereira DR, Silva-Correia J, Oliveira JM, et al. Hydrogels in acellular and cellular strategies for intervertebral disc regeneration [J]. J Tissue Eng Regen Med. 2013;7(2):85–98.CrossRef
18.
Gonzalez JS, Alvarez VA. Mechanical properties of polyvinylalcohol/hydroxyapatite cryogel as potential artificial cartilage [J]. J Mech Behav Biomed Mater. 2014;34:47–56.CrossRef
19.
Dormer NH, Singh M, Wang L, et al. Osteochondral interface tissue engineering using macroscopic gradients of bioactive signals [J]. Ann Biomed Eng. 2010;38(6):2167–82.CrossRef
20.
Bailey BM, Nail LN, Grunlan MA. Continuous gradient scaffolds for rapid screening of cell-material interactions and interfacial tissue regeneration [J]. Acta Biomater. 2013;9(9):8254–61.CrossRef
21.
Khandan A, Ozada N, Saber-Samandari S, Nejad MG. On the mechanical and biological properties of bredigite-magnetite (Ca7MgSi4O16-Fe3O4) nanocomposite scaffolds. Ceramics International 2018;44(3):3141–8.CrossRef
22.
Lin HY, Tsai WC, Chang SH. Collagen-PVA aligned nanofiber on collagen sponge as bi-layered scaffold for surface cartilage repair. J Biomater Sci Polym Ed. 2017;28(7):664–78.CrossRef
23.
Nie L, Zhang G, Hou R, Xu H, Li Y, Fu J. Controllable promotion of chondrocyte adhesion and growth on PVA hydrogels by controlled release of TGF-β1 from porous PLGA microspheres. Colloids Surf B Biointerfaces. 2015;125:51–7.CrossRef
24.
Song W, Markel DC, Wang S, Shi T, Mao G, Ren W. Electrospun polyvinyl alcohol-collagen-hydroxyapatite nanofibers: a biomimetic extracellular matrix for osteoblastic cells. Nanotechnology. 2012;23(11):115101.CrossRef
25.
Dormer NH, Singh M, Zhao L, et al. Osteochondral interface regeneration of the rabbit knee with macroscopic gradients of bioactive signals [J]. J Biomed Mater Res A. 2012;100(1):162–70.CrossRef
26.
AN EK, Saber-Samandari S. Fabrication of hydroxyapatite-baghdadite nanocomposite scaffolds coated by PCL/Bioglass with polyurethane polymeric sponge technique [J]. Nanomedicine J. 2017;4(3):177–83.
27.
JX MZ, SL ML, YH WS. Design and evaluation of poly (lactic-co-glyclic acid)/poly (vinyl alcohol)/nano-hydroxyapatite hydrogels for cartilage tissue engineering in vitro [J]. Int J Clin Exp Med. 2016;9(6):9817–27.
28.
SS AK, Nejad MG. Preparation of novel porous calcium silicate scaffold loaded by celecoxib drug using freeze drying technique: fabrication, characterization and simulation [J]. Ceramics. 2019;45(11):14126–35.CrossRef
29.
AH MF, NAA ASH. Synergistic effects of chitosan scaffold and TGFβ1 on the proliferation and osteogenic differentiation of dental pulp stem cells derived from human exfoliated [J]. Arch Oral Biol. 2014;59(12):1400–11.CrossRef
30.
Wang T, Lai JH, Yang F. Effects of hydrogel stiffness and extracellular compositions on modulating cartilage regeneration by mixed populations of stem cells and chondrocytes in vivo [J]. Tissue Eng Part A. 2016;22(23–24):1348–56.CrossRef
31.
Sen KS, Duarte Campos DF, Köpf M, et al. The effect of addition of calcium phosphate particles to hydrogel-based composite materials on stiffness and differentiation of Mesenchymal stromal cells toward Osteogenesis [J]. Adv Healthc Mater. 2018;7(18):e1800343.CrossRef
32.
Subramanian A, Krishnan UM, Sethuraman S. In vivo biocompatibility of PLGA-polyhexylthiophene nanofiber scaffolds in a rat model [J]. Biomed Res Int. 2013;2013:390518.CrossRef
33.
Zhang HX, Xiao GY, Wang X, et al. Biocompatibility and osteogenesis of calcium phosphate composite scaffolds containing simvastatin-loaded PLGA microspheres for bone tissue engineering [J]. J Biomed Mater Res A. 2015;103(10):3250–8.CrossRef
34.
Nava MM, Draghi L, Giordano C, et al. The effect of scaffold pore size in cartilage tissue engineering [J]. J Appl Biomater Funct Mater. 2016;14(3):e223–9.PubMed
35.
Engler AJ, Sen S, Sweeney HL, et al. Matrix elasticity directs stem cell lineage specification [J]. Cell. 2006;126(4):677–89.CrossRef
36.
Little CJ, Bawolin NK, Chen X. Mechanical properties of natural cartilage and tissue-engineered constructs [J]. Tissue Eng Part B Rev. 2011;17(4):213–27.CrossRef
37.
Blum MM, Ovaert TC. Low friction hydrogel for articular cartilage repair: evaluation of mechanical and tribological properties in comparison with natural cartilage tissue [J]. Mater Sci Eng C Mater Biol Appl. 2013;33(7):4377–83.CrossRef
Metadata
Title
Design and evaluation of nano-hydroxyapatite/poly(vinyl alcohol) hydrogels coated with poly(lactic-co-glycolic acid)/nano-hydroxyapatite/poly(vinyl alcohol) scaffolds for cartilage repair
Authors
Weiping Su
Yihe Hu
Min Zeng
Mingqing Li
Shaoru Lin
Yangying Zhou
Jie Xie
Publication date
01-12-2019
Publisher
BioMed Central
Published in
Journal of Orthopaedic Surgery and Research / Issue 1/2019
Electronic ISSN: 1749-799X
DOI
https://doi.org/10.1186/s13018-019-1450-0

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