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Journal of Orthopaedic Surgery and Research
Published in: Journal of Orthopaedic Surgery and Research 1/2019

Open Access 01-12-2019 | Research article

Evaluating the bioactivity of a hydroxyapatite-incorporated polyetheretherketone biocomposite

Authors: Rui Ma, Dagang Guo

Published in: Journal of Orthopaedic Surgery and Research | Issue 1/2019

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Abstract

Background

Polyetheretherketone (PEEK) exhibits stable chemical properties, excellent biocompatibility, and rational mechanical properties that are similar to those of human cortical bone, but the lack of bioactivity impedes its clinical application.

Methods

In this study, hydroxyapatite (HA) was incorporated into PEEK to fabricate HA/PEEK biocomposite using a compounding and injection-molding technique. The tensile properties of the prepared HA/PEEK composites (HA content from 0 to 40 wt%) were tested to choose an optimal HA content. To evaluate the bioactivity of the composite, the cell attachment, proliferation, spreading and alkaline phosphatase (ALP) activity of MC3T3-E1 cells, and apatite formation after immersion in simulated body fluid (SBF), and osseointegration in a rabbit cranial defect model were investigated. The results were compared to those from ultra-high molecular weight polyethylene (UHMWPE) and pure PEEK.

Results

By evaluating the tensile properties and elastic moduli of PEEK composite samples/PEEK composites with different HA contents, the 30 wt% HA/PEEK composite was chosen for use in the subsequent tests. The results of the cell tests demonstrated that PEEK composite samples/PEEK composite exhibited better cell attachment, proliferation, spreading, and higher ALP activity than those of UHMWPE and pure PEEK. Apatite islands formed on the HA/PEEK composite after immersion in SBF for 7 days and grew continuously with longer time periods. Animal tests indicated that bone contact and new bone formation around the HA/PEEK composite were more obvious than those around UHMWPE and pure PEEK.

Conclusions

The HA/PEEK biocomposite created by a compounding and injection-molding technique exhibited enhanced osteogenesis and could be used as a candidate of orthopedic implants.
Literature
1.
Kurtz SM, Devine JN. PEEK biomaterials in trauma, orthopedic, and spinal implants. Biomaterials. 2007;28:4845–69.CrossRef
2.
Jarman-Smith M. Evolving uses for implantable PEEK and PEEK based compounds. Med Device Technol. 2008;19:12–5.PubMed
3.
Ma R, Tang T. Current strategies to improve the bioactivity of PEEK. Int J Mol Sci. 2014;15:5426–45.CrossRef
4.
Wang H, Xu M, Zhang W, Kwok DT, Jiang J, Wu Z, et al. Mechanical and biological characteristics of diamond-like carbon coated poly aryl-ether-ether-ketone. Biomaterials. 2010;31:8181–7.CrossRef
5.
Briem D, Strametz S, Oder K, Meenen N, Lehmann W, Linhart W, et al. Response of primary fibroblasts and osteoblasts to plasma treated polyetheretherketone(PEEK) surfaces. J Mater Sci: Mater M. 2005;16:671–7.
6.
Ryan KR, Gabriel LC, Robert JK, Weimin Y. Hydroxyapatite-reinforced polymer biocomposites for synthetic bone substitutes. JOM. 2008;60:38–45.
7.
Kokubo T, Kim H-M, Kawashita M. Novel bioactive materials with different mechanical properties. Biomaterials. 2003;24:2161–75.CrossRef
8.
Hollinger JO, Battistone GC. Biodegradable bone repair materials. Synthetic polymers and ceramics. Clin Orthop Relat Res. 1986;207:290–305.
9.
Hammerle CH, Olah AJ, Schmid J, Fluckiger L, Gogolewski S, Winkler JR, et al. The biological effect of natural bone mineral on bone neoformation on the rabbit skull. Clin Oral Implants Res. 1997;8:198–207.CrossRef
10.
Bonfield W, Grynpas MD, Tully AE, Bowman J, Abram J. Hydroxyapatite reinforced polyethylene-a mechanically compatible implant material for bone replacement. Biomaterials. 1981;2:185–6.CrossRef
11.
Wang M, Porter D, Bonfield W. Processing, characterization, and evaluation of hydroxyapatite reinforced polyethylene composites. Brit Ceram T. 1994;93:91–5.
12.
Zhang Y, Hao L, Savalani MM, Harris RA, Di Silvio L, Tanner KE. In vitro biocompatibility of hydroxyapatite-reinforced polymeric composites manufactured by selective laser sintering. J Biomed Mater Res A. 2009;91:1018–27.CrossRef
13.
Yu S, Hariram KP, Kumar R, Cheang P, Aik KK. In vitro apatite formation and its growth kinetics on hydroxyapatite/polyetheretherketone biocomposites. Biomaterials. 2005;26:2343–52.CrossRef
14.
Ma R, Weng L, Bao X, Ni Z, Song S, Cai W. Characterization of in situ synthesized hydroxyapatite/polyetheretherketone composite materials. Mater Lett. 2012;71:117–9.CrossRef
15.
Ma R, Weng L, Bao X, Song S, Zhang Y. In vivo biocompatibility and bioactivity of in situ synthesized hydroxyapatite/polyetheretherketone composite materials. J Appl Polym Sci. 2013;127:2581–7.CrossRef
16.
Bakar MSA, Cheang P, Khor KA. Tensile properties and microstructural analysis of spheroidized hydroxyapatite/poly(etheretherketone) biocomposites. Mat Sci Eng A. 2003;345:55–63.CrossRef
17.
Lee JH, Jang HL, Lee KM, Baek HR, Jin K, Hong KS, et al. In vitro and in vivo evaluation of the bioactivity of hydroxyapatite-coated polyetheretherketone biocomposites created by cold spray technology. Acta Biomater. 2013;9:6177-87.
18.
D638-01 AS. Standard test method for tensile properties of plastics. West Conshohocken, PA, USA: American Society for Testing and Materials; 2001.
19.
Fan Q, Tang T, Zhang X, Dai K. The role of CCAAT/enhancer binding protein (C/EBP)-alpha in osteogenesis of C3H10T1/2 cells induced by BMP-2. J Cel Mol Med. 2009;13:2489–505.CrossRef
20.
Sun H, Wu C, Dai K, Chang J, Tang T. Proliferation and osteoblastic differentiation of human bone marrow-derived stromal cells on akermanite-bioactive ceramics. Biomaterials. 2006;27:5651–7.CrossRef
21.
Kokubo T, Takadama H. How useful is SBF in predicting in vivo bone bioactivity? Biomaterials. 2006;27:2907–15.CrossRef
22.
Hench LL, Wilson J. In: Hench LL, Wilson J, editors. An introduction to bioceramics. Singapore: World Scientific; 1993. p. 12.CrossRef
23.
Han CM, Lee EJ, Kim HE, Koh YH, Kim KN, Ha Y, et al. The electron beam deposition of titanium on polyetheretherketone (PEEK) and the resulting enhanced biological properties. Biomaterials. 2010;31:3465–70.CrossRef
24.
Santhosh S, Balasivanandha Prabu S. Thermal stability of nano hydroxyapatite synthesized from sea shells through wet chemical synthesis. Mater Lett. 2013;97:121–4.CrossRef
25.
Toth JM, Wang M, Estes BT, Scifert JL, Seim HB 3rd, Turner AS. Polyetheretherketone as a biomaterial for spinal applications. Biomaterials. 2006;27:324–34.CrossRef
26.
Boccaccini AR, Blaker JJ. Bioactive composite materials for tissue engineering scaffolds. Expert Rev Med Devi. 2005;2:303–17.CrossRef
27.
Bakar MSA, Cheang P, Khor KA. Mechanical properties of injection molded hydroxyapatite-polyetheretherketone biocomposites. Compos Sci Technol. 2003;63:421–5.CrossRef
28.
Tan H, Guo S, Yang S, Xu X, Tang T. Physical characterization and osteogenic activity of the quaternized chitosan-loaded PMMA bone cement. Acta Biomater. 2012;8:2166–74.CrossRef
29.
Hu X, Neoh KG, Shi Z, Kang ET, Poh C, Wang W. An in vitro assessment of titanium functionalized with polysaccharides conjugated with vascular endothelial growth factor for enhanced osseointegration and inhibition of bacterial adhesion. Biomaterials. 2010;31:8854–63.CrossRef
30.
Noiset O, Schneider YJ, Marchand-Brynaert J. Fibronectin adsorption or/and covalent grafting on chemically modified PEEK film surfaces. J Biomater Sci Polym E. 1999;10:657–77.CrossRef
31.
Bodhak S, Bose S, Bandyopadhyay A. Role of surface charge and wettability onearly stage mineralization and bone cell-materials interactions of polarizedhydroxyapatite. Acta Biomater. 2009;5:2178–88.CrossRef
32.
Yang F, Wolke JGC, Jansen JA. Biomimetic calcium phosphate coating onelectrospun poly(e-caprolactone) scaffolds for bone tissue engineering. Chem Eng J. 2008;137:154–61.CrossRef
33.
Ito Y, Hasuda H, Kamitakahara M, Ohtsuki C, Tanihara M, Kang IK. Acomposite of hydroxyapatite with electrospun biodegradable nanofibers as atissue engineering material. J Biosci Bioeng. 2005;100:43–9.CrossRef
34.
Thamaraiselvi TV, Rajeswari S. Biological evaluation of bioceramic materials-a review. Trends Biomater Artif Organs. 2004;18:9-17.
35.
Ruan J, Grant MH. Biocompatibility evaluation in vitro. Part I: Morphology expression and proliferation of human and rat osteoblasts on the biomaterials. J Cent South Univ T. 2001;8:1–8.CrossRef
36.
Yuan H, Kurashina K, de Bruijn JD, Li Y, de Groot K, Zhang X. A preliminary study on osteoinduction of two kinds of calcium phosphate ceramics. Biomaterials. 1999;20:1799–806.CrossRef
37.
Christenson EM, Anseth KS, van den Beucken JJ, Chan CK, Ercan B, Jansen JA, et al. Nanobiomaterial applications in orthopedics. J Orthop Res. 2007;25:11–22.CrossRef
38.
Hench LL, Splinter RJ, Allen W, Greenlee T. Bonding mechanisms at the interface of ceramic prosthetic materials. J Biomed Mater Res. 1971;5:117–41.CrossRef
39.
Lee JH, Jang HL, Lee KM, Baek HR, Jin K, Hong KS, et al. In vitro and in vivo evaluation of the bioactivity of hydroxyapatite-coated polyetheretherketone biocomposites created by cold spray technology. Acta Biomater. 2013;9:6177–87.CrossRef
40.
Ohtsuki C, Kushitani H, Kokubo T, Kotani S, Yamamuro T. Apatite formation on the surface of Ceravital-type glass-ceramic in the body. J Biomed Mater Res. 1991;25:1363–70.CrossRef
Metadata
Title
Evaluating the bioactivity of a hydroxyapatite-incorporated polyetheretherketone biocomposite
Authors
Rui Ma
Dagang Guo
Publication date
01-12-2019
Publisher
BioMed Central
Published in
Journal of Orthopaedic Surgery and Research / Issue 1/2019
Electronic ISSN: 1749-799X
DOI
https://doi.org/10.1186/s13018-019-1069-1

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