Top

Journal of Orthopaedic Surgery and Research

Published in:

Open Access 01-12-2017 | Research article

Effect of modified compound calcium phosphate cement on the differentiation and osteogenesis of bone mesenchymal stem cells

Authors: Jican Zeng, Jiazhong Lin, Guanfeng Yao, Kangmei Kong, Xinjia Wang

Published in: Journal of Orthopaedic Surgery and Research | Issue 1/2017

Abstract

Background

The aim of this study is to evaluate the effect of self-invented compound calcium phosphate cement upon the proliferation and osteogenesis of bone mesenchymal stem cells (BMSCs).

Methods

Four groups including traditional calcium phosphate cement, modified calcium phosphate cement, modified calcium phosphate cement plus bone morphogenetic protein (BMP), and control groups were established. The cell proliferation curve was delineated by MTT. The activity of BMSCs to synthesize alkaline phosphatase (AKP) was evaluated. The growth and invasion of BMSCs were observed. The expression levels of aggrecan, collagen I, collagen II, AKP, and OSX messenger RNA (mRNA) were measured by using RT-PCR.

Results

Compared with other groups, the BMSCs in the modified calcium phosphate cement group presented with loose microstructure and the BMSCs closely attached to the vector margin. At 7 days after co-culture, the expression of AKP in the modified calcium phosphate cement plus BMP group was significantly upregulated compared with those in other groups. In the modified calcium phosphate cement group, the BMSCs properly proliferated on the surface of bone cement and invaded into the cement space. At 10 days, the expression levels of aggrecan, collagen I, collagen II, AKP, and OSX mRNA in the modified calcium phosphate cement and modified calcium phosphate cement plus BMP groups were significantly upregulated than those in other groups.

Conclusions

Modified compound calcium phosphate cement possesses excellent biocompatibility and osteogenic induction ability. Loose microstructure and large pore size create a favorable environment for BMSCs proliferation and vascular invasion, as an ideal vector for releasing BMP cytokines to mediate the differentiation and osteogenesis of BMSCs.

Weitao Y, Kangmei K, Xinjia W, Weili Q. Bone regeneration using an injectable calcium phosphate/autologous iliac crest bone composites for segmental ulnar defects in rabbits. J Mater Sci Mater Med. 2008;19:2485–92.CrossRefPubMed

Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-delta delta C(T)) method. Methods. 2001;25:402–8.CrossRefPubMed

Fernández E, Vlad MD, Gel MM, López J, Torres R, Cauich JV, et al. Modulation of porosity in apatitic cements by the use of alpha-tricalcium phosphate-calcium sulphatedihy dratemixtures. Biomaterials. 2005;26:3395–404.CrossRefPubMed

Theiss F, Apelt D, Brand B, Kutter A, Zlinszky K, Bohner M, et al. Biocompatibility and resorption of a brushite calcium phosphate cement. Biomaterials. 2005;26:4383–94.CrossRefPubMed

Flatley T, Lynch K, Benson M. Tissue response to implants of calcium phosphate ceremic in the rabbit spine. Clin Orthop. 1983;179:246–50.CrossRef

Martin TJ, Sims NA. Os teoclas t-derived activity in the coupling of bone formation to resorption. Trends Mol Med. 2005;11:76–81.CrossRefPubMed

Reddi AH, Huggins C. Biochemical sequences in the transformation of normal fibroblasts in adolescent rats. Proc Natl Acad Sci USA. 1972;69:1601–5.CrossRefPubMedPubMedCentral

Grosfeld EC, Hoekstra JW, Herber RP, Ulrich DJ, Jansen JA, van den Beucken JJ. Long-term biological performance of injectable and degradable calcium phosphate cement. Biomed Mater. 2016;12:015009.CrossRefPubMed

Axrap A, Wang J, Liu Y, Wang M, Yusuf A. Study on adhesion, proliferation and differentiation of osteoblasts promoted by new absorbable bioactive glass injection in vitro. Eur Rev Med Pharmacol Sci. 2016;20:4677–81.PubMed

10.

Karageorgiou V, Kaplan D. Porosity of 3d biomaterial scaffolds and osteogenesis. Biomaterials. 2005;26:5474–91.CrossRefPubMed

11.

Gosain AK, Song L, Riordan P, Amarante MT, Nagy PG, Wilson CR, et al. A 1-year study of osteoinduction in hydroxyapatite-derived biomaterials in an adult sheep model: part I. Plast Reconstr Surg. 2002;109:619–30.CrossRefPubMed

12.

Yuan H, Li Y, de Bruijn JD, de Groot K, Zhang X. Tissue responses of calcium phosphate cement: a study in dogs. Biomaterials. 2000;21:1283–90.CrossRefPubMed

13.

Renno AC, Nejadnik MR, van de Watering FC, Crovace MC, Zanotto ED, Hoefnagels JP, et al. Incorporation of bioactive glass in calcium phosphate cement: material characterization and in vitro degradation. J Biomed Mater Res A. 2013;101:2365–73.CrossRefPubMed

14.

Ginebra MP, Canal C, Espanol M, de Groot K, Zhang X. Calcium phosphate cements as drug delivery materials. Adv Drug Deliv Rev. 2012;64:1090–110.CrossRefPubMed

15.

Hoppe A, Guldal NS, Boccaccini AR. A review of the biological response to ionic dissolution products from bioactive glasses and glass-ceramics. Biomaterials. 2011;32:2757–74.CrossRefPubMed

Title: Effect of modified compound calcium phosphate cement on the differentiation and osteogenesis of bone mesenchymal stem cells
Authors: Jican Zeng
Jiazhong Lin
Guanfeng Yao
Kangmei Kong
Xinjia Wang
Publication date: 01-12-2017
Publisher: BioMed Central
Published in: Journal of Orthopaedic Surgery and Research / Issue 1/2017
Electronic ISSN: 1749-799X
DOI: https://doi.org/10.1186/s13018-017-0598-8

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Effect of modified compound calcium phosphate cement on the differentiation and osteogenesis of bone mesenchymal stem cells

Abstract

Background

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

Please log in to get access to this content

Other articles of this Issue 1/2017

Biomechanical properties of 3D-printed bone scaffolds are improved by treatment with CRFP

The correlation between the high-intensity zone on a T2-weighted MRI and positive outcomes of discography: a meta-analysis

Long-term results and comparison of the three different high tibial osteotomy and fixation techniques in medial compartment arthrosis

Subscapularis- and deltoid-sparing vs traditional deltopectoral approach in reverse shoulder arthroplasty: a prospective case-control study

Application of different patella height indices in patients undergoing total knee arthroplasty

Arthroscopic internal drainage and cystectomy of popliteal cyst in knee osteoarthritis