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Diagnostic Pathology

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Open Access 01-12-2020 | Glioma | Research

Gli1 regulates stemness characteristics in gastric adenocarcinoma

Authors: Wenbo Qi, Zhaoting Yang, Ying Feng, Haoyue Li, Nan Che, Lan Liu, Yanhua Xuan

Published in: Diagnostic Pathology | Issue 1/2020

Abstract

Background

Glioma-associated oncogene homolog 1 (Gli1), affects the progression and the stemness characteristics of malignant carcinoma. The aim of the present study was to identify the relation between Glioma-associated oncogene homolog 1 (Gli1) and stemness and determine its clinical significance in gastric adenocarcinoma (GA). We investigated Gli1 expression and its correlation with other stemness-associated proteins in 169 GA samples and 5 GA cell lines.

Methods

To elucidate the role of Gli1 in the clinicopathological significance and stemness of GA, tissues samples from 169 GA patients were collected for immunohistochemistry (IHC). Additionally, MKN74, MKN28, NCI-N87, SNU638, AGS cells were collected for western blotting, MKN28 cells were collected for spheroid formation assay.

Results

Results showed that Gli1 expression was closely related to tumor grade, primary tumor (pT) stage, distant metastasis, clinical stage, gross type, microvessel density, and shorter overall survival (OS). Cox regression analysis verified that Gli1 was an independent prognostic factor for OS. Furthermore, Gli1 expression correlated with the expression of stemness-related genes, CD44, LSD1, and Sox9. Gli1 inhibitor GANT61 significantly decreased the expression of CD44 and LSD1, and spheroid formation ability of the MKN28 cells.

Conclusions

In conclusion, Gli1 may be a poor prognostic indicator and a potential cancer stemness-related protein in GA.

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Title: Gli1 regulates stemness characteristics in gastric adenocarcinoma
Authors: Wenbo Qi
Zhaoting Yang
Ying Feng
Haoyue Li
Nan Che
Lan Liu
Yanhua Xuan
Publication date: 01-12-2020
Publisher: BioMed Central
Keywords: Glioma
Metastasis
Glioma
Glioma
Published in: Diagnostic Pathology / Issue 1/2020
Electronic ISSN: 1746-1596
DOI: https://doi.org/10.1186/s13000-020-00949-5

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Gli1 regulates stemness characteristics in gastric adenocarcinoma

Abstract

Background

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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