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Open Access 01-12-2019 | Pathology | Research

Three-dimensional histopathological reconstruction of bladder tumours

Authors: Ilaria Jansen, Marit Lucas, C. Dilara Savci-Heijink, Sybren L. Meijer, Esmee I. M. L. Liem, Onno J. de Boer, Ton G. van Leeuwen, Henk A. Marquering, Daniel M. de Bruin

Published in: Diagnostic Pathology | Issue 1/2019

Abstract

Background

Histopathological analysis is the cornerstone in bladder cancer (BCa) diagnosis. These analysis suffer from a moderate observer agreement in the staging of bladder cancer. Three-dimensional reconstructions have the potential to support the pathologists in visualizing spatial arrangements of structures, which may improve the interpretation of specimen. The aim of this study is to present three-dimensional (3D) reconstructions of histology images.

Methods

En-bloc specimens of transurethral bladder tumour resections were formalin fixed and paraffin embedded. Specimens were cut into sections of 4 μm and stained with Hematoxylin and Eosin (H&E). With a Phillips IntelliSite UltraFast scanner, glass slides were digitized at 20x magnification. The digital images were aligned by performing rigid and affine image alignment. The tumour and the muscularis propria (MP) were manually delineated to create 3D segmentations. In conjunction with a 3D display, the results were visualized with the Vesalius3D interactive visualization application for a 3D workstation.

Results

En-bloc resection was performed in 21 BCa patients. Per case, 26–30 sections were included for the reconstruction into a 3D volume. Five cases were excluded due to export problems, size of the dataset or condition of the tissue block. Qualitative evaluation suggested an accurate registration for 13 out of 16 cases. The segmentations allowed full 3D visualization and evaluation of the spatial relationship of the BCa tumour and the MP.

Conclusion

Digital scanning of en-bloc resected specimens allows a full-fledged 3D reconstruction and analysis and has a potential role to support pathologists in the staging of BCa.

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Title: Three-dimensional histopathological reconstruction of bladder tumours
Authors: Ilaria Jansen
Marit Lucas
C. Dilara Savci-Heijink
Sybren L. Meijer
Esmee I. M. L. Liem
Onno J. de Boer
Ton G. van Leeuwen
Henk A. Marquering
Daniel M. de Bruin
Publication date: 01-12-2019
Publisher: BioMed Central
Keyword: Pathology
Published in: Diagnostic Pathology / Issue 1/2019
Electronic ISSN: 1746-1596
DOI: https://doi.org/10.1186/s13000-019-0803-7

At a glance: The STEP trials

Springer Medicine

Three-dimensional histopathological reconstruction of bladder tumours

Abstract

Background

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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