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Diagnostic Pathology
Published in: Diagnostic Pathology 1/2015

Open Access 01-12-2015 | Research

RETRACTED ARTICLE: Up-regulation of miR-130b expression level and down-regulation of miR-218 serve as potential biomarker in the early detection of human osteosarcoma

Authors: Afshin Taheriazam, Amir Jouya Talaei, Mohammad Jamshidi, Mohammadreza Shakeri, Samaneh Khoshbakht, Emad Yahaghi, Marjan Shokrani

Published in: Diagnostic Pathology | Issue 1/2015

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Abstract

Background

Osteosarcoma (OS) is a primary malignant bone tumor with high morbidity that principally emerges in children and adolescents. MiRNAs regulate a variety of normal physiologic processes and are involved in tumorigenesis and development of multiple malignancies, including OS. This study was aimed to evaluate the clinical significance of miR-130b and miR-218 in osteosarcoma patient.

Methods

We utilized quantitative real-time PCR to evaluate the level of miR-130b and miR-218 expressions in OS patients and normal tissues and their relationship with clinicopathological features and survival in OS patients.

Results

QRT-PCR indicated that miR-130b expression in tumor tissues was strongly elevated than adjacent non-tumor tissues (P < 0.001), while the level of miR-218 expression in osteosarcoma tissues was down-regulated than adjacent non-tumor tissues (P < 0.001). We evaluated the clinical significance of miR-130b and miR-218 in osteosarcoma. Clinical correlation analysis showed that increased expression of miR-130b and decreased expression of miR-218 were significantly associated with advanced tumor stage (x 2 = 6.285, P < 0.009; x 2 = 7.172, P < 0.007), distant metastasis (x 2  = 5.528; P < 0.001; x 2 = 4.617, P < 0.001) and size of tumor (x 2 = 5.01, P = 0.013; x 2 = 4.271, P = 0.019).

Conclusions

Taken together, our data indicated that high miR-130b level and low level of miR-218 are associated with poor clinicopathological characteristics. Furthermore, miR-130b may play a key role in the progression of osteosarcoma.
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Metadata
Title
RETRACTED ARTICLE: Up-regulation of miR-130b expression level and down-regulation of miR-218 serve as potential biomarker in the early detection of human osteosarcoma
Authors
Afshin Taheriazam
Amir Jouya Talaei
Mohammad Jamshidi
Mohammadreza Shakeri
Samaneh Khoshbakht
Emad Yahaghi
Marjan Shokrani
Publication date
01-12-2015
Publisher
BioMed Central
Published in
Diagnostic Pathology / Issue 1/2015
Electronic ISSN: 1746-1596
DOI
https://doi.org/10.1186/s13000-015-0422-x

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