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Open Access 01-12-2015 | Research

Study on expression of lncRNA RGMB-AS1 and repulsive guidance molecule b in non-small cell lung cancer

Authors: Ping Li, Juan Li, Rui Yang, Furui Zhang, Huaqi Wang, Heying Chu, Yao Lu, Shaozhi Dun, Yuanyuan Wang, Wenqiao Zang, Yuwen Du, Xiaonan Chen, Guoqiang Zhao, Guojun Zhang

Published in: Diagnostic Pathology | Issue 1/2015

Abstract

Background

The relationships between lncRNAs and tumors have currently become one of the focuses on cancer studies. However, there are a few studies about lncRNAs in non-small cell lung cancer (NSCLC) at present.

Methods

Microarray analysis was designed to study the expression patterns of lncRNAs in three pairs of NSCLC tissues. The expression of lncRNA RGMB-AS1 and repulsive guidance molecule b (RGMB) were detected in 72 paired NSCLC tissues and adjacent normal tissues by qRT-PCR assay. The relations of lncRNA RGMB-AS1 and RGMB expression with clinicopathological factors of NSCLC patients were explored. A549 and SPC-A-1 cells were transfected with siRNA of lncRNA RGMB-AS1 and negative control. RGMB expression level was detected by qRT-PCR assay and western blot analysis.

Results

The results of microarray found that 571 lncRNAs were differentially expressed in NSCLC tissues (Fold change cut-off: 5.0, P < 0.05), including 304 upregulated and 267 downregulated lncRNAs. The results of qRT-PCR showed that lncRNA RGMB-AS1 expression was significantly higher in NSCLC tissues than in adjacent normal tissues (P < 0.05), while RGMB mRNA showed an opposite trend (P < 0.05). Correlation analysis indicated that the expression of lncRNA RGMB-AS1and RGMB mRNA were inversely correlated (R² = 0.590, P < 0.05). While lncRNA RGMB-AS1 and RGMB expression levels in NSCLC tissues were associated with the occurrence of differentiation status, lymph node metastases and TNM stage (P < 0.05). Transfection with siRNA of lncRNA RGMB-AS1, subsequent results showed that RGMB mRNA and protein expression were upregulated (P < 0.05) in A549 and SPC-A-1 cells compared to the control groups.

Conclusion

We identified lncRNA RGMB-AS1 was upregulated and RGMB was downregulated in NSCLC patients. Both were related to differentiation status, lymph node metastases and TNM stage. Studies also indicated that lncRNA RGMB-AS1and RGMB were inversely correlated.

Virtual slides

The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/7911587521528276

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Title: Study on expression of lncRNA RGMB-AS1 and repulsive guidance molecule b in non-small cell lung cancer
Authors: Ping Li
Juan Li
Rui Yang
Furui Zhang
Huaqi Wang
Heying Chu
Yao Lu
Shaozhi Dun
Yuanyuan Wang
Wenqiao Zang
Yuwen Du
Xiaonan Chen
Guoqiang Zhao
Guojun Zhang
Publication date: 01-12-2015
Publisher: BioMed Central
Published in: Diagnostic Pathology / Issue 1/2015
Electronic ISSN: 1746-1596
DOI: https://doi.org/10.1186/s13000-015-0297-x

At a glance: The STEP trials

Springer Medicine

Study on expression of lncRNA RGMB-AS1 and repulsive guidance molecule b in non-small cell lung cancer

Abstract

Background

Methods

Results

Conclusion

Virtual slides

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

Virtual slides

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