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Open Access 01-12-2018 | Short paper

Zika virus crosses an in vitro human blood brain barrier model

Authors: Judie B. Alimonti, Maria Ribecco-Lutkiewicz, Caroline Sodja, Anna Jezierski, Danica B. Stanimirovic, Qing Liu, Arsalan S. Haqqani, Wayne Conlan, Mahmud Bani-Yaghoub

Published in: Fluids and Barriers of the CNS | Issue 1/2018

Abstract

Zika virus (ZIKV) is a flavivirus that is highly neurotropic causing congenital abnormalities and neurological damage to the central nervous systems (CNS). In this study, we used a human induced pluripotent stem cell (iPSC)-derived blood brain barrier (BBB) model to demonstrate that ZIKV can infect brain endothelial cells (i-BECs) without compromising the BBB barrier integrity or permeability. Although no disruption to the BBB was observed post-infection, ZIKV particles were released on the abluminal side of the BBB model and infected underlying iPSC-derived neural progenitor cells (i-NPs). AXL, a putative ZIKV cellular entry receptor, was also highly expressed in ZIKV-susceptible i-BEC and i-NPs. This iPSC-derived BBB model can help elucidate the mechanism by which ZIKV can infect BECs, cross the BBB and gain access to the CNS.

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Title: Zika virus crosses an in vitro human blood brain barrier model
Authors: Judie B. Alimonti
Maria Ribecco-Lutkiewicz
Caroline Sodja
Anna Jezierski
Danica B. Stanimirovic
Qing Liu
Arsalan S. Haqqani
Wayne Conlan
Mahmud Bani-Yaghoub
Publication date: 01-12-2018
Publisher: BioMed Central
Published in: Fluids and Barriers of the CNS / Issue 1/2018
Electronic ISSN: 2045-8118
DOI: https://doi.org/10.1186/s12987-018-0100-y

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