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Journal of the International Society of Sports Nutrition
Published in: Journal of the International Society of Sports Nutrition 1/2017

Open Access 01-12-2017 | Research article

Enzymatically modified isoquercitrin supplementation intensifies plantaris muscle fiber hypertrophy in functionally overloaded mice

Authors: Akiko Kohara, Masanao Machida, Yuko Setoguchi, Ryouichi Ito, Masanori Sugitani, Hiroko Maruki-Uchida, Hiroyuki Inagaki, Tatsuhiko Ito, Naomi Omi, Tohru Takemasa

Published in: Journal of the International Society of Sports Nutrition | Issue 1/2017

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Abstract

Background

Enzymatically modified isoquercitrin (EMIQ) is produced from rutin using enzymatic hydrolysis followed by treatment with glycosyltransferase in the presence of dextrin to add glucose residues. EMIQ is absorbed in the same way as quercetin, a powerful antioxidant reported to prevent disused muscle atrophy by targeting mitochondria and to have ergogenic effects. The present study investigated the effect of EMIQ on skeletal muscle hypertrophy induced by functional overload.

Methods

In Study 1, 6-week-old ICR male mice were divided into 4 groups: sham-operated control, sham-operated EMIQ, overload-operated control, and overload-operated EMIQ groups. In Study 2, mice were divided into 3 groups: overload-operated whey control, overload-operated whey/EMIQ (low dose), and overload-operated whey/EMIQ (high dose) groups. The functional overload of the plantaris muscle was induced by ablation of the synergist (gastrocnemius and soleus) muscles. EMIQ and whey protein were administered with food. Three weeks after the operation, the cross-sectional area and minimal fiber diameter of the plantaris muscle fibers were measured.

Results

In Study 1, functional overload increased the cross-sectional area and minimal fiber diameter of the plantaris muscle. EMIQ supplementation significantly increased the cross-sectional area and minimal fiber diameter of the plantaris muscle in both the sham-operated and overload-operated groups. In Study 2, EMIQ supplementation combined with whey protein administration significantly increased the cross-sectional area and minimal fiber diameter of the plantaris muscle.

Conclusion

EMIQ, even when administered as an addition to whey protein supplementation, significantly intensified the fiber hypertrophy of the plantaris muscle in functionally overloaded mice. EMIQ supplementation also induced fiber hypertrophy of the plantaris in sham-operated mice.
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Metadata
Title
Enzymatically modified isoquercitrin supplementation intensifies plantaris muscle fiber hypertrophy in functionally overloaded mice
Authors
Akiko Kohara
Masanao Machida
Yuko Setoguchi
Ryouichi Ito
Masanori Sugitani
Hiroko Maruki-Uchida
Hiroyuki Inagaki
Tatsuhiko Ito
Naomi Omi
Tohru Takemasa
Publication date
01-12-2017
Publisher
BioMed Central
DOI
https://doi.org/10.1186/s12970-017-0190-y

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