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Journal of Cardiovascular Magnetic Resonance
Published in: Journal of Cardiovascular Magnetic Resonance 1/2017

Open Access 01-12-2016 | Research

Myocardial T1 maps reflect histological findings in acute and chronic stages of myocarditis in a rat model

Authors: Sarah Jeuthe, Katharina Wassilew, Darach O h-Ici, Tiago Ferreira da Silva, Frédéric Münch, Felix Berger, Titus Kuehne, Burkert Pieske, Daniel R. Messroghli

Published in: Journal of Cardiovascular Magnetic Resonance | Issue 1/2017

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Abstract

Background

Cardiovascular magnetic resonance offers both diagnostic and prognostic information in myocarditis. Using an established animal model of myocarditis, the aim of this study was to measure myocardial T1 before the onset, in the acute and in the chronic phases of the disease and to compare its course with histological and immunohistochemistry findings.

Methods

Male young Lewis rats were immunized with 0.25 mg porcine myocardial myosin into the rear footpads on day 0. Native and contrast-enhanced ECG-triggered cardiac MRI examinations were performed before immunization on day 0 and on days 14, 21 and 35. Left ventricular function, pre- and post- contrast T1 parameters and LGE images were assessed using Small animal look-locker inversion recovery (SALLI). For each of the indicated time points a minimum of 4 rats were randomly sacrificed for pathological investigations including conventional histology (HE and Sirius-Red staining) and immunohistochemistry (CD 68) investigations.

Results

All immunized rats developed myocarditis (morbidity 100 %). Histologically we observed increased wall thickness with biventricular macrophage-rich mixed inflammatory infiltrates. All rats with a histologically severe myocarditis showed increased native T1 and decreased post-contrast T1 of the myocardium.

Conclusions

The assessment of native T1 and post-contrast T1 allows accurate differentiation between healthy myocardium and myocardium with inflammation and also between the acute and chronic phases of the disease.
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Metadata
Title
Myocardial T1 maps reflect histological findings in acute and chronic stages of myocarditis in a rat model
Authors
Sarah Jeuthe
Katharina Wassilew
Darach O h-Ici
Tiago Ferreira da Silva
Frédéric Münch
Felix Berger
Titus Kuehne
Burkert Pieske
Daniel R. Messroghli
Publication date
01-12-2016
Publisher
BioMed Central
Published in
Journal of Cardiovascular Magnetic Resonance / Issue 1/2017
Electronic ISSN: 1532-429X
DOI
https://doi.org/10.1186/s12968-016-0241-6

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