Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The ONWARDS insulin icodec trials

A round-up of the ONWARDS phase 3 clinical trials evaluating the novel weekly insulin icodec in people with diabetes.
ADVANCED SEARCH
Log in
Top
Reproductive Biology and Endocrinology
Published in: Reproductive Biology and Endocrinology 1/2017

Open Access 01-12-2017 | Research

Evaluation of three-dimensional SonoAVC ultrasound for antral follicle count in infertile women: its agreement with conventional two-dimensional ultrasound and serum levels of anti-Müllerian hormone

Authors: P. A. Peres Fagundes, R. Chapon, P. R. Olsen, A. K. Schuster, M. M. C. Mattia, J. S. Cunha-Filho

Published in: Reproductive Biology and Endocrinology | Issue 1/2017

Login to get access

Abstract

Background

Several studies have reported a correlation between antral follicle count by conventional 2D transvaginal sonography and serum anti-Müllerian hormone levels. However, few studies have investigated the effectiveness of 3D SonoAVC transvaginal ultrasound technology, particularly in infertile women. Therefore, this study aims to evaluate the usefulness of three-dimensional (3D) SonoAVC transvaginal ultrasound technology for antral follicle count and its correlation to conventional two-dimensional (2D) transvaginal ultrasound and serum levels of anti-Müllerian hormone in infertile women.

Methods

This cross-sectional study included 42 infertile women with age lower than 40 years that underwent treatment at a private fertility clinic between June and December 2015. Patient data included age, body mass index and cause of infertility. On cycle day 3 the following hormone levels were measured: serum levels of anti-Müllerian hormone, follicle-stimulating hormone, cancer antigen 125, prolactin, thyroid-stimulating hormone and oestradiol; the number of antral follicles was counted as well. The scanning were performed through 2D and 3D technology transvaginal ultrasound.

Results

Using a Bland-Altman test we demonstrated that both technologies are quite equivalent. However, antral follicle count is higher using 3D ultrasound technology compared to 2D technology (p < 0.001; Wilcoxon test), this finding is mainly remarkable in ovaries with more than 20 antral follicles. Moreover, the mean time required for manual 2D ultrasound and 3D SonoAVC measurements were 275 ± 109 and 103 ± 57 s, respectively (p < 0.001). Serum AMH concentration correlated to the total number of early antral follicles (correlation coefficients = 0.678 and 0.612; p < 0.001 by 2D ultrasound and 3D SonoAVC, respectively; Spearman’s correlation test).

Conclusions

Antral follicle count is better estimated using 3D ultrasound compared to 2D technology. A great advantage of 3D SonoAVC was less time required for an examination and the visual advantage when it need to count more than 20 follicles.

Trial registration

CAAE:​ 35141114.​4.​0000.​5327. Registered 10 June 2015.
Literature
1.
La Marca A, Muttukrishna S, Cunha-Filho JS, et al. Age-related normograms of serum antimüllerian hormone levels in a population of infertile women: a multicenter study. Fertil Steril. 2011;95:2359–63.CrossRefPubMed
2.
de Vet A, Laven JSE, de Jong FH, Themmen APN, Fauser BC. Anti-müllerian hormone serum levels: a putative marker for ovarian aging. Fertil Steril. 2002;77:357–62.CrossRefPubMed
3.
Bonilla-Musoles F, Castillo JC, Caballero O, et al. Predicting ovarian reserve and reproductive outcome using antimüllerian hormone (AMH) and antral follicle count (AFC) in patients with previous assisted reproduction technique (ART) failure. Clin Exp Obstet Gynecol. 2012;39:13–8.PubMed
4.
Fanchin R, Schonauer LM, Righini C, Guibourdenche J, Frydman R, Taieb J. Serum anti-Müllerian hormone is more strongly related to ovarian folicular status than serum innibin B, oestradiol, FSH and LH on day 3. Hum Reprod. 2003;18:323–7.CrossRefPubMed
5.
Jayaprakasan K, Hilwah N, Kendall NR, et al. Does 3D ultrasound offer any advantage in the pretreatment assessment of ovarian reserve and prediction of outcome after assisted reproduction treatment. Hum Reprod. 2007;22:1932–41.CrossRefPubMed
6.
Venturella R, Lico D, Sarica A, et al. OvAge: a new methodology to quantify ovarian reserve combining clinical, biochemical and 3D-ultrasonographic parameters. J Ovarian Res. 2015;8:21.CrossRefPubMedPubMedCentral
7.
Bland JM, Altman DG. Statistical methods for assessing agreement between two methods of clinical measurement. Lancet. 1986;1:307–10.CrossRefPubMed
8.
Nylander M, et al. Ovarian morphology in polycystic ovarian syndrome: estimates from 2D and 3D ultrasound and magnetic resonance imaging and their correlation to anti-Müllerian hormone. Acta Radiol. 2017;58(8):997–1004.CrossRefPubMed
9.
Weenen C, et al. Anti-Müllerian hormone expression pattern in human ovary: potential implications for initial and cyclic follicle recruitment. Mol Hum Reprod. 2004;10(2):77–83.CrossRefPubMed
10.
Deb SR, Campbell BK, Cleves J, Porter N, Winter B, Raine-Fenning N. Quantitative analysis of number and size of antral follicles: a comparison of real-time two-dimensional and automated three-dimensional ultrasound. Ultrasound Obstet Gynecol. 2010;35:354–60.CrossRefPubMed
11.
Raine-Fenning N, Jayaprakasan K, Clewes J. Automated follicle tracking facilitates standardization and may improve work flow. Ultrasound Obstet Gynecol. 2007;30:1015–8.CrossRefPubMed
12.
Kyei-Mensah A, Zaidi J, Pittrof R, Shaker A, Campbell S, Tan SL. Transvaginal three dimensional ultrasound: accuracy of follicular volume measurements. Fertil Steril. 1996;65:371–6.CrossRefPubMed
13.
Deb S, Jayaprakasan K, Campbell BK, Clewes JS, Johnson IR, Raine-Fenning NJ. Intraobserver and interobserver reliability of automated antral follicle counts made using three-dimensional ultrasound and SonoAVC. Ultrasound Obstet Gynecol. 2009;33:477–83.CrossRefPubMed
Metadata
Title
Evaluation of three-dimensional SonoAVC ultrasound for antral follicle count in infertile women: its agreement with conventional two-dimensional ultrasound and serum levels of anti-Müllerian hormone
Authors
P. A. Peres Fagundes
R. Chapon
P. R. Olsen
A. K. Schuster
M. M. C. Mattia
J. S. Cunha-Filho
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Reproductive Biology and Endocrinology / Issue 1/2017
Electronic ISSN: 1477-7827
DOI
https://doi.org/10.1186/s12958-017-0314-x

Other articles of this Issue 1/2017

Reproductive Biology and Endocrinology 1/2017 Go to the issue

Research

Does daily co-administration of letrozole and gonadotropins during ovarian stimulation improve IVF outcome?

Research

Differential gene expression profiling of endometrium during the mid-luteal phase of the estrous cycle between a repeat breeder (RB) and non-RB cows

Research

Towards a better understanding of preimplantation genetic screening for aneuploidy: insights from a virtual trial for women under the age of 40 when transferring embryos one at a time

Research

Low-dose lymphocyte immunotherapy rebalances the peripheral blood Th1/Th2/Treg paradigm in patients with unexplained recurrent miscarriage

Research

Effect of race and ethnicity on utilization and outcomes of assisted reproductive technology in the USA

Research

Serum periostin levels in early in pregnancy are significantly altered in women with miscarriage