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Reproductive Biology and Endocrinology
Published in: Reproductive Biology and Endocrinology 1/2017

Open Access 01-12-2017 | Research

Proteomic analysis of human follicular fluid associated with successful in vitro fertilization

Authors: Xiaofang Shen, Xin Liu, Peng Zhu, Yuhua Zhang, Jiahui Wang, Yanwei Wang, Wenting Wang, Juan Liu, Ning Li, Fujun Liu

Published in: Reproductive Biology and Endocrinology | Issue 1/2017

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Abstract

Background

Human follicular fluid (HFF) provides a key environment for follicle development and oocyte maturation, and contributes to oocyte quality and in vitro fertilization (IVF) outcome.

Methods

To better understand folliculogenesis in the ovary, a proteomic strategy based on dual reverse phase high performance liquid chromatography (RP-HPLC) coupled to matrix-assisted laser desorption/ionization time-of-flight tandem mass spectrometry (LC-MALDI TOF/TOF MS) was used to investigate the protein profile of HFF from women undergoing successful IVF.

Results

A total of 219 unique high-confidence (False Discovery Rate (FDR) < 0.01) HFF proteins were identified by searching the reviewed Swiss-Prot human database (20,183 sequences), and MS data were further verified by western blot. PANTHER showed HFF proteins were involved in complement and coagulation cascade, growth factor and hormone, immunity, and transportation, KEGG indicated their pathway, and STRING demonstrated their interaction networks. In comparison, 32% and 50% of proteins have not been reported in previous human follicular fluid and plasma.

Conclusions

Our HFF proteome research provided a new complementary high-confidence dataset of folliculogenesis and oocyte maturation environment. Those proteins associated with innate immunity, complement cascade, blood coagulation, and angiogenesis might serve as the biomarkers of female infertility and IVF outcome, and their pathways facilitated a complete exhibition of reproductive process.
Appendix
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Metadata
Title
Proteomic analysis of human follicular fluid associated with successful in vitro fertilization
Authors
Xiaofang Shen
Xin Liu
Peng Zhu
Yuhua Zhang
Jiahui Wang
Yanwei Wang
Wenting Wang
Juan Liu
Ning Li
Fujun Liu
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Reproductive Biology and Endocrinology / Issue 1/2017
Electronic ISSN: 1477-7827
DOI
https://doi.org/10.1186/s12958-017-0277-y

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