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Reproductive Biology and Endocrinology
Published in: Reproductive Biology and Endocrinology 1/2017

Open Access 01-12-2017 | Research

Chronic endometritis modifies decidualization in human endometrial stromal cells

Authors: Di Wu, Fuminori Kimura, Luyi Zheng, Mitsuaki Ishida, Yoko Niwa, Kimiko Hirata, Akie Takebayashi, Akiko Takashima, Kentaro Takahashi, Ryoji Kushima, Guangmei Zhang, Takashi Murakami

Published in: Reproductive Biology and Endocrinology | Issue 1/2017

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Abstract

Background

Chronic endometritis (CE) is a continuous inflammation of uterine endometrium, and it is usually symptomless. As CE has been thought not to affect the reproductive status and general health of affected women, its significance has not been explored. However, recent studies have shown that CE is related with repeated implantation failures after in vitro fertilization-embryo transfer, unexplained infertility, and recurrent miscarriages. As decidua differentiates to support the implantation process and maintains the pregnancy, we hypothesized that CE may influence the process of decidualization.

Methods

Seventeen patients were employed in the experiment involving culture of endometrial stromal cells (ESCs). After obtaining endometrial samples, ESCs were harvested and cultured for 13 days. The concentrations in culture media and the protein expressions in ESCs of prolactin (PRL) and insulin-like growth factor binding protein-1 (IGFBP-1), two well known decidualization markers used in a large number of in vitro models, were analyzed by ELISA and Western blotting, respectively, and the cell numbers were also counted. The mRNA levels of PRL and IGFBP-1 were tested by quantitative real time polymerase chain reaction (RT-PCR). Since sex hormone induce proliferation and differentiation to decidua via binding to the sex hormone receptors (ERα, ERβ, PRA, and PRB), their expression was assessed in another 17 patients’ paraffin-embedded endometrial tissue specimens by immunohistochemistry and semi-quantified by H-score.

Results

Increased cell numbers and reduced secretion of PRL and IGFBP-1 were detected by ELISA in the ESCs of CE patients after culture for 13 days compared with non-CE patients. The decreased protein expression of IGFBP-1 in ESCs of CE patients was detected by Western blotting. The decreased expression of PRL mRNA and IGFBP-1 mRNA were detected by RT-PCR. Increased expressions of ERα, ERβ, PRA, and PRB were observed in the stromal cells of CE patients in comparison to non-CE patients, whereas increased expressions of ERα and ERβ were detected in the glandular cells of CE.

Conclusion

Our data suggests that CE modifies decidualization of human ESC through untuning the function of sex steroid hormone receptor.
Appendix
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Metadata
Title
Chronic endometritis modifies decidualization in human endometrial stromal cells
Authors
Di Wu
Fuminori Kimura
Luyi Zheng
Mitsuaki Ishida
Yoko Niwa
Kimiko Hirata
Akie Takebayashi
Akiko Takashima
Kentaro Takahashi
Ryoji Kushima
Guangmei Zhang
Takashi Murakami
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Reproductive Biology and Endocrinology / Issue 1/2017
Electronic ISSN: 1477-7827
DOI
https://doi.org/10.1186/s12958-017-0233-x

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