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Reproductive Biology and Endocrinology
Published in: Reproductive Biology and Endocrinology 1/2015

Open Access 01-12-2015 | Research

Metabolites involved in cellular communication among human cumulus-oocyte-complex and sperm during in vitro fertilization

Authors: María José Gómez-Torres, Eva María García, Jaime Guerrero, Sonia Medina, María José Izquierdo-Rico, Ángel Gil-Izquierdo, Jesús Orduna, María Savirón, Leopoldo González-Brusi, Jorge Ten, Rafael Bernabeu, Manuel Avilés

Published in: Reproductive Biology and Endocrinology | Issue 1/2015

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Abstract

Background

Fertilization is a key physiological process for the preservation of the species. Consequently, different mechanisms affecting the sperm and the oocyte have been developed to ensure a successful fertilization. Thus, sperm acrosome reaction is necessary for the egg coat penetration and sperm-oolema fusion. Several molecules are able to induce the sperm acrosome reaction; however, this process should be produced coordinately in time and in the space to allow the success of fertilization between gametes.
The goal of this study was to analyze the metabolites secreted by cumulus-oocyte-complex (COC) to find out new components that could contribute to the induction of the human sperm acrosome reaction and other physiological processes at the time of gamete interaction and fertilization.

Methods

For the metabolomic analysis, eighteen aliquots of medium were used in each group, containing: a) only COC before insemination and after 3 h of incubation; b) COC and capacitated spermatozoa after insemination and incubated for 16–20 hours; c) only capacitated sperm after 16–20 h in culture and d) only fertilization medium as control. Six patients undergoing assisted reproduction whose male partners provided normozoospermic samples were included in the study. Seventy-two COC were inseminated.

Results

The metabolites identified were monoacylglycerol (MAG), lysophosphatidylcholine (LPC) and phytosphingosine (PHS). Analysis by PCR and in silico of the gene expression strongly suggests that the cumulus cells contribute to the formation of the PHS and LPC.

Conclusions

LPC and PHS are secreted by cumulus cells during in vitro fertilization and they could be involved in the induction of human acrosome reaction (AR). The identification of new molecules with a paracrine effect on oocytes, cumulus cells and spermatozoa will provide a better understanding of gamete interaction.
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Metadata
Title
Metabolites involved in cellular communication among human cumulus-oocyte-complex and sperm during in vitro fertilization
Authors
María José Gómez-Torres
Eva María García
Jaime Guerrero
Sonia Medina
María José Izquierdo-Rico
Ángel Gil-Izquierdo
Jesús Orduna
María Savirón
Leopoldo González-Brusi
Jorge Ten
Rafael Bernabeu
Manuel Avilés
Publication date
01-12-2015
Publisher
BioMed Central
Published in
Reproductive Biology and Endocrinology / Issue 1/2015
Electronic ISSN: 1477-7827
DOI
https://doi.org/10.1186/s12958-015-0118-9

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