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Open Access 01-12-2017 | Research

Monitoring multiple myeloma by idiotype-specific peptide binders of tumor-derived exosomes

Authors: Enrico Iaccino, Selena Mimmi, Vincenzo Dattilo, Fabiola Marino, Patrizio Candeloro, Antonio Di Loria, Danilo Marimpietri, Antonio Pisano, Francesco Albano, Eleonora Vecchio, Simona Ceglia, Gaetanina Golino, Antonio Lupia, Giuseppe Fiume, Ileana Quinto, Giuseppe Scala

Published in: Molecular Cancer | Issue 1/2017

Abstract

Tumor-derived exosomes (TDEs) play a pivotal role in tumor establishment and progression, and are emerging biomarkers for tumor diagnosis in personalized medicine. To date, there is a lack of efficient technology platforms for exosome isolation and characterization. Multiple myeloma (MM) is an incurable B-cell malignancy due to the rapid development of drug-resistance. MM-released exosomes express the immunoglobulin B-cell receptor (Ig-BCR) of the tumor B-cells, which can be targeted by Idiotype-binding peptides (Id-peptides). In this study, we analyzed the production of MM-released exosomes in the murine 5T33MM multiple myeloma model as biomarkers of tumor growth. To this end, we selected Id-peptides by screening a phage display library using as bait the Ig-BCR expressed by 5T33MM cells. By FACS, the FITC-conjugated Id-peptides detected the MM-released exosomes in the serum of 5T33MM-engrafted mice, levels of which are correlated with tumor progression at an earlier time point compared to serum paraprotein. These results indicate that Id-peptide-based recognition of MM-released exosomes may represent a very sensitive diagnostic approach for clinical evaluation of disease progression.

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Title: Monitoring multiple myeloma by idiotype-specific peptide binders of tumor-derived exosomes
Authors: Enrico Iaccino
Selena Mimmi
Vincenzo Dattilo
Fabiola Marino
Patrizio Candeloro
Antonio Di Loria
Danilo Marimpietri
Antonio Pisano
Francesco Albano
Eleonora Vecchio
Simona Ceglia
Gaetanina Golino
Antonio Lupia
Giuseppe Fiume
Ileana Quinto
Giuseppe Scala
Publication date: 01-12-2017
Publisher: BioMed Central
Published in: Molecular Cancer / Issue 1/2017
Electronic ISSN: 1476-4598
DOI: https://doi.org/10.1186/s12943-017-0730-8

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