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Cancer Cell International
Published in: Cancer Cell International 1/2019

Open Access 01-12-2019 | Glioblastoma | Primary research

SWAP-70 promotes glioblastoma cellular migration and invasion by regulating the expression of CD44s

Authors: Lin Shi, Huize Liu, Yifeng Wang, Yulong Chong, Jie Wang, Guanzheng Liu, Xu Zhang, Xiangyu Chen, Huan Li, Mingshan Niu, Jun Liang, Rutong Yu, Xuejiao Liu

Published in: Cancer Cell International | Issue 1/2019

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Abstract

Background

Switch-associated protein 70 (SWAP-70) is a guanine nucleotide exchange factor that is involved in cytoskeletal rearrangement and regulation of migration and invasion of malignant tumors. However, the mechanism by which SWAP-70 regulates the migration and invasion of glioblastoma (GB) cells has not been fully elucidated.

Methods

This study used an online database to analyze the relationship between SWAP-70 expression and prognosis in GB patients. The in vitro wound healing assay and transwell invasion assay were used to determine the role of SWAP-70 in GB cell migration and invasion as well as the underlying mechanism.

Results

We found that patients with high SWAP-70 expression in the GB had a poor prognosis. Downregulation of SWAP-70 inhibited GB cell migration and invasion, whereas SWAP-70 overexpression had an opposite effect. Interestingly, SWAP-70 expression was positively correlated with the expression of the standard form of CD44 (CD44s) in GB tissues. Downregulation of SWAP-70 also reduced CD44s protein expression, whereas SWAP-70 overexpression enhanced CD44s protein expression. However, downregulation of SWAP-70 expression did not affect the mRNA expression of CD44s. Reversal experiments showed that overexpressing CD44s in cell lines with downregulated SWAP-70 partially abolished the inhibitory effects of downregulated SWAP-70 on GB cell migration and invasion.

Conclusions

These results suggest that SWAP-70 may promote GB cell migration and invasion by regulating the expression of CD44s. SWAP-70 may serve as a new biomarker and a potential therapeutic target for GB.
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Metadata
Title
SWAP-70 promotes glioblastoma cellular migration and invasion by regulating the expression of CD44s
Authors
Lin Shi
Huize Liu
Yifeng Wang
Yulong Chong
Jie Wang
Guanzheng Liu
Xu Zhang
Xiangyu Chen
Huan Li
Mingshan Niu
Jun Liang
Rutong Yu
Xuejiao Liu
Publication date
01-12-2019
Publisher
BioMed Central
Published in
Cancer Cell International / Issue 1/2019
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/s12935-019-1035-3

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