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Cancer Cell International
Published in: Cancer Cell International 1/2018

Open Access 01-12-2018 | Primary research

Paraoxonase 3 is involved in the multi-drug resistance of esophageal cancer

Authors: Dabing Huang, Yong Wang, Yifu He, Gang Wang, Wei Wang, Xinghua Han, Yubei Sun, Lin Lin, Benjie Shan, Guodong Shen, Min Cheng, Geng Bian, Xiang Fang, Shilian Hu, Yueyin Pan

Published in: Cancer Cell International | Issue 1/2018

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Abstract

Background

Drug resistance prevents the effective treatment of cancers. DNA methylation has been found to participate in the development of cancer drug resistance.

Methods

We performed the wound-healing and invasion assays to test the effect of the paraoxonase gene PON3 on esophageal cancer (EC) cells. In addition, in vivo EC-derived tumor xenografts in nude mice were generated to test the effect of PON3 on the chemoresistance of EC cells.

Results

We found that PON3 is hypermethylated in drug-resistant EC cell line K150, which in-return down-regulates its expression. The following experiments by the forced changes of PON3 level in vitro and in vivo demonstrated that the PON3 expression negatively correlates with drug resistance in EC cells. Further wound-healing and invasion assays showed that PON3 suppresses the migration and invasion of EC cells.

Conclusion

Our data established that PON3 is associated with the EC drug resistance, which may serve as a biomarker for the potential therapeutic treatment of EC.
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Metadata
Title
Paraoxonase 3 is involved in the multi-drug resistance of esophageal cancer
Authors
Dabing Huang
Yong Wang
Yifu He
Gang Wang
Wei Wang
Xinghua Han
Yubei Sun
Lin Lin
Benjie Shan
Guodong Shen
Min Cheng
Geng Bian
Xiang Fang
Shilian Hu
Yueyin Pan
Publication date
01-12-2018
Publisher
BioMed Central
Published in
Cancer Cell International / Issue 1/2018
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/s12935-018-0657-1

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