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Cancer Cell International
Published in: Cancer Cell International 1/2018

Open Access 01-12-2018 | Primary Research

DNA methylation affects metastasis of renal cancer and is associated with TGF-β/RUNX3 inhibition

Authors: Jianbo Zheng, Yanhui Mei, Ping Xiang, Guangsheng Zhai, Ning Zhao, Chuanbing Xu, Min Liu, Zhengsheng Pan, Kai Tang, Dongsheng Jia

Published in: Cancer Cell International | Issue 1/2018

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Abstract

Background

Renal cell carcinoma accounts for 2–3% of all cancers and metastasis increased the malignancy of renal cancer. However, the role of methylation in metastasis of renal cancer is poorly understood.

Methods

We performed targeted gene array to compare the differential expressions of methylation regulated genes in metastatic and primary renal cancer tissues. Quantitative methylation specific PCR was performed to examine the CpG methylation levels of Runt related transcription factor 3 (RUNX3) and transforming growth factor (TGF)-β. Western blot was performed to detect the expression of target genes. Murine xenograft renal cancer model was established to assay gene expression, methylation level, tumor growth and animal survival in vivo.

Results

RUNX3 and TGF-β levels were decreased in metastatic renal cancer tissues as a result of their CpG methylation. Metastatic xenograft model displayed decreased expression levels of RUNX3 and TGF-β and higher CpG methylation levels, bigger tumor size and shorter survival time, all which were restored by treatment with a methylation inhibitor.

Conclusions

Hypermethylation in CpG islands promotes metastasis of renal cancer and is associated with TGF-β and RUNX3 inhibition.
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Metadata
Title
DNA methylation affects metastasis of renal cancer and is associated with TGF-β/RUNX3 inhibition
Authors
Jianbo Zheng
Yanhui Mei
Ping Xiang
Guangsheng Zhai
Ning Zhao
Chuanbing Xu
Min Liu
Zhengsheng Pan
Kai Tang
Dongsheng Jia
Publication date
01-12-2018
Publisher
BioMed Central
Published in
Cancer Cell International / Issue 1/2018
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/s12935-018-0554-7

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