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Cancer Cell International

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Open Access 01-12-2017 | Primary Research

High brain acid soluble protein 1(BASP1) is a poor prognostic factor for cervical cancer and promotes tumor growth

Authors: Huiru Tang, Yan Wang, Bing Zhang, Shiqiu Xiong, Liangshuai Liu, Wei Chen, Guosheng Tan, Heping Li

Published in: Cancer Cell International | Issue 1/2017

Abstract

Background

The aim of this study was to determine whether brain abundant membrane attached signal protein 1 (BASP1) is a valuable prognostic biomarker for cervical cancer and whether BASP1 regulates the progression of cervical cancer.

Methods

Quantitative real-time PCR, western blotting, and immunohistochemistry were used to determined BASP1 levels. Statistical analyses were used to examine whether BASP1 was a prognostic factor for patients with cervical cancer. The MTT assay, colony formation assay, cell cycle assay, anchorage-independent growth assay, and a tumor xenograft model were used to determine the role of BASP1 in the proliferation and tumorigenicity of cervical cancer.

Results

Brain abundant membrane attached signal protein 1 was upregulated in cervical cancer tissues and cells, and BASP1 expression levels were higher in patients that had died during follow-up compared with those that survived. There was a positive correlation between BASP1 expression and clinical stage (p < 0.001), T classification (p < 0.001), N classification (p < 0.05), and survival or mortality (p < 0.05). Patients with higher BASP1 expression had a shorter overall survival time. Cox regression analysis shown BSAP1 was an unfavorable prognostic factor for patients with cervical cancer. Overexpression of BASP1 promoted the proliferation of cervical cancer and its colony formation ability, accelerated cell cycle progression, and enhanced tumorgenicity. BASP1 knockdown inhibited the proliferation of cervical cancer and its colony formation ability, suppressed cell cycle progression, and decreased tumorgenicity.

Conclusions

The results showed that BASP1 not only is a novel prognostic factor for patients with cervical cancer, but also promotes the proliferation and tumorigenicity of cervical cancer.

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Title: High brain acid soluble protein 1(BASP1) is a poor prognostic factor for cervical cancer and promotes tumor growth
Authors: Huiru Tang
Yan Wang
Bing Zhang
Shiqiu Xiong
Liangshuai Liu
Wei Chen
Guosheng Tan
Heping Li
Publication date: 01-12-2017
Publisher: BioMed Central
Published in: Cancer Cell International / Issue 1/2017
Electronic ISSN: 1475-2867
DOI: https://doi.org/10.1186/s12935-017-0452-4

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Abstract

Background

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