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Cancer Cell International
Published in: Cancer Cell International 1/2017

Open Access 01-12-2017 | Primary research

Overexpression of trefoil factor 3 (TFF3) contributes to the malignant progression in cervical cancer cells

Authors: Zhaohu Yuan, Dandan Chen, Xiaojie Chen, Huikuan Yang, Yaming Wei

Published in: Cancer Cell International | Issue 1/2017

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Abstract

Background

There remains a great need for effective therapies for cervical cancers, the majority of which are aggressive leaving patients with poor prognosis.

Methods and results

Here, we identify a novel candidate therapeutic target, trefoil factor 3 (TFF3) which overexpressed in cervical cancer cells and was associated with reduced postoperative survival. Functional studies demonstrated that TFF3 overexpression promoted the proliferation and invasion of cervical cancer cells, and inhibited the apoptosis by inducing the mRNA changes in SiHa and Hela cell lines. Conversely, TFF3 silencing disrupted the proliferation and invasion of cervical cancer cells, and induced the apoptosis via Click-iT EdU test, flow cytometry analysis and two-dimensional Matrigel Transwell analysis. Western blot analysis showed that overexpression of TFF3 repressed E-cadherin (CDH1) expression to promote the invasion of cervical cancer cells. Furthermore, down-regulated CDH1 via overexpression of TFF3 was significantly up-regulated by virtue of inhibitor of p-STAT3.

Conclusions

These results suggested that TFF3 stimulated the invasion of cervical cancer cells probably by activating the STAT3/CDH1 signaling pathway. Furthermore, overexpression of TFF3 decreased the sensitivity of cervical cancer cells to etoposide by increasing P-glycoprotein (P-gp) functional activity. Overall, our work provides a preclinical proof that TFF3 not only contributes to the malignant progression of cervical cancers and but also is a potential therapeutic target.
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Metadata
Title
Overexpression of trefoil factor 3 (TFF3) contributes to the malignant progression in cervical cancer cells
Authors
Zhaohu Yuan
Dandan Chen
Xiaojie Chen
Huikuan Yang
Yaming Wei
Publication date
01-12-2017
Publisher
BioMed Central
Published in
Cancer Cell International / Issue 1/2017
Electronic ISSN: 1475-2867
DOI
https://doi.org/10.1186/s12935-016-0379-1

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