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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2018

Open Access 01-12-2018 | Research article

Antioxidant and skin-whitening effects of aerial part of Euphorbia supina Raf. Extract

Authors: Sa-Haeng Kang, Yong-Deok Jeon, Ji-Yoon Cha, Sung-Woo Hwang, Hoon-Yeon Lee, Min Park, Bo-Ri Lee, Min-Kyoung Shin, Su-Jeong Kim, Sang-Min Shin, Dae-Ki Kim, Jong-Sik Jin, Young-Mi Lee

Published in: BMC Complementary Medicine and Therapies | Issue 1/2018

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Abstract

Background

Euphorbia supina (ES) has been widely used in folk medicine owing to its antibacterial, hemostatic, and anti-inflammatory properties. The aim of this study was to evaluate the antioxidant and skin-whitening effects of a 70% ethanol extract of ES.

Methods

The aerial parts of ES plant were extracted with 70% ethanol. The viability of B16F10 cells was evaluated by MTT assay to determine the non-toxic doses for further experiments. The tyrosinase and cellular tyrosinase activities were then measured using an enzyme-substrate assay. In addition, the expression of whitening-related proteins was measured using western blot.

Results

The antioxidant activity of the ES samples increased in a dose-dependent manner, as confirmed by their radical scavenging activities in the 2,2-diphenyl-1-1-picrylhydrazyl and 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) assays. The ES extract significantly reduced tyrosinase activity and melanin content in a dose-dependent manner. Furthermore, it decreased α-melanocyte stimulating hormone (MSH)-induced protein expression of tyrosinase and microphthalmia-associated transcription factor (MITF).

Conclusions

Our results indicate that the ES extract attenuated α-MSH-stimulated melanin synthesis by modulating tyrosinase and MITF expression. Therefore, the ES extract could be a promising therapeutic agent to treat hyperpigmentation and as an ingredient for skin-whitening cosmetics.
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Metadata
Title
Antioxidant and skin-whitening effects of aerial part of Euphorbia supina Raf. Extract
Authors
Sa-Haeng Kang
Yong-Deok Jeon
Ji-Yoon Cha
Sung-Woo Hwang
Hoon-Yeon Lee
Min Park
Bo-Ri Lee
Min-Kyoung Shin
Su-Jeong Kim
Sang-Min Shin
Dae-Ki Kim
Jong-Sik Jin
Young-Mi Lee
Publication date
01-12-2018
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2018
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-018-2323-5

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