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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2018

Open Access 01-12-2018 | Research article

Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent

Authors: Isabel Cristina Celerino de Moraes Porto, Dayse Chaves Cardoso de Almeida, Gabriela Vasconcelos Calheiros de Oliveira Costa, Tayná Stéphanie Sampaio Donato, Letícia Moreira Nunes, Ticiano Gomes do Nascimento, José Marcos dos Santos Oliveira, Carolina Batista da Silva, Natanael Barbosa dos Santos, Maria Luísa de Alencar e Silva Leite, Irinaldo Diniz Basílio-Júnior, Camila Braga Dornelas, Pierre Barnabé Escodro, Eduardo Jorge da Silva Fonseca, Regianne Umeko Kamiya

Published in: BMC Complementary Medicine and Therapies | Issue 1/2018

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Abstract

Background

Propolis is a natural substance produced by bees and is known to have antimicrobial activity. Our aim was to evaluate the antimicrobial effect of micellar nanocomposites loaded with an ethyl acetate extract of Brazilian red propolis as a cavity cleaning agent and its influence on the color and microtensile bond strength (μTBS) of the dentin/resin interface.

Methods

An ultra-performance liquid chromatography coupled with a diode array detector (UPLC-DAD) assay was used to determine the flavonoids and isoflavones present in an ethyl acetate extract of Brazilian red propolis (EARP) and micellar nanocomposites loaded with EARP (MNRP). The antimicrobial activity of EARP and MNRP was tested against Streptococcus mutans, Lactobacillus acidophilus, and Candida albicans. One of the following experimental treatments was applied to etched dentin (phosphoric acid, 15 s): 5 μL of MNRP (RP3, 0.3%; RP6, 0.6%; or RP1, 1.0% w/v), placebo, and 2% chlorhexidine digluconate. Single Bond adhesive (3 M/ESPE) was applied and a 4-mm-thick resin crown (Z350XT, 3 M/ESPE) was built up. After 24 h, the teeth were sectioned into sticks for the μTBS test and scanning electron microscopy. Spectrophotometry according to the CIE L*a*b* chromatic space was used to evaluate the color. Data were analyzed using one-way ANOVA and the Tukey test or Kruskal-Wallis test and the same test for pairwise comparisons between the means (P < 0.05).

Results

The UPLC-DAD assay identified the flavonoids liquiritigenin, pinobanksin, pinocembrin, and isoliquiritigenin and the isoflavonoids daidzein, formononetin, and biochanin A in the EARP and micellar nanocomposites. EARP and MNRP presented antimicrobial activity against the cariogenic bacteria Streptococcus mutans and Lactobacillus acidophilus, and for Candida albicans. ΔE values varied from 2.31 to 3.67 (P = 0.457). The mean μTBS for RP1 was significantly lower than for the other groups (P < 0.001). Dentin treated with RP1 showed the shortest resin tags followed by RP6 and RP3.

Conclusions

The EARP and (MNRP) showed antimicrobial activity for the main agents causing dental caries (Streptococcus mutans and Lactobacillus acidophilus) and for Candida albicans. MNRP at concentrations of 0.3 and 0.6% used as a cavity cleaner do not compromise the aesthetics or μTBS of the dentin/resin interface.
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Metadata
Title
Mechanical and aesthetics compatibility of Brazilian red propolis micellar nanocomposite as a cavity cleaning agent
Authors
Isabel Cristina Celerino de Moraes Porto
Dayse Chaves Cardoso de Almeida
Gabriela Vasconcelos Calheiros de Oliveira Costa
Tayná Stéphanie Sampaio Donato
Letícia Moreira Nunes
Ticiano Gomes do Nascimento
José Marcos dos Santos Oliveira
Carolina Batista da Silva
Natanael Barbosa dos Santos
Maria Luísa de Alencar e Silva Leite
Irinaldo Diniz Basílio-Júnior
Camila Braga Dornelas
Pierre Barnabé Escodro
Eduardo Jorge da Silva Fonseca
Regianne Umeko Kamiya
Publication date
01-12-2018
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2018
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-018-2281-y

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