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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2018

Open Access 01-12-2018 | Research article

Immunomodulatory properties of quercetin-3-O-α-L-rhamnopyranoside from Rapanea melanophloeos against influenza a virus

Authors: Parvaneh Mehrbod, Muna Ali Abdalla, Fatemeh Fotouhi, Masoumeh Heidarzadeh, Abimbola O. Aro, Jacobus N. Eloff, Lyndy J. McGaw, Folorunso O. Fasina

Published in: BMC Complementary Medicine and Therapies | Issue 1/2018

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Abstract

Background

Influenza infection is a major public health threat. The role of influenza A virus-induced inflammatory response in severe cases of this disease is widely recognized. Drug resistance and side effects of chemical treatments have been observed, resulting in increased interest in alternative use of herbal medications for prophylaxis against this infection.
The South African medicinal plant, Rapanea melanophloeos (RM) (L.) Mez of the family Myrsinaceae was selected owing to its traditional use for the treatment of several diseases such as respiratory ailments and also previous preliminary studies of anti-influenza activity of its methanolic extract. The aim of this study was to investigate the immunomodulatory properties of a glycoside flavone isolated from RM against influenza A virus.

Methods

The non-cytotoxic concentration of the quercetin-3-O-α-L-rhamnopyranoside (Q3R) was determined by MTT assay and tested for activity against influenza A virus (IAV) in simultaneous, pre-penetration and post-penetration combination treatments over 1 h incubation on MDCK cells. The virus titer and viral load targeting NP and M2 viral genes were determined using HA and qPCR, respectively. TNF-α and IL-27 as pro- and anti-inflammatory cytokines were measured at RNA and protein levels by qPCR and ELISA, respectively.

Results

Quercetin-3-O-α-L-rhamnopyranoside at 150 μg/ml decreased the viral titer by 6 logs (p < 0.01) in the simultaneous procedure. The NP and M2 genes copy numbers as viral target genes, calculated based on the Ct values and standard formula, significantly decreased in simultaneous treatment (p < 0.01). The expression of cytokines was also considerably affected by the compound treatment.

Conclusions

This is the first report of quercetin-3-O-α-L-rhamnopyranoside from RM and its immunomodulatory properties against influenza A virus. Further research will focus on detecting the specific mechanism of virus-host interactions.
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Literature
1.
Webster R, Bean WGO, Chambers T, Kawaoka Y. Evolution and ecology of influenza a viruses. Microbiol Rev. 1992;56:152–79.PubMedPubMedCentral
2.
Fedson DS. Confronting an influenza pandemic with inexpensive generic agents: can it be done? Lancet Infect Dis. 2008;8:571–6.CrossRefPubMed
3.
Pathumwadee I, Chittima L, Thanyada R, Arthorn L, Maturos M, Panita D, et al. How amantadine and rimantadine inhibit proton transport in the M2 protein channel. J Mol Graph Model. 2008;27:342–8.CrossRef
4.
Fiore AE, Shay DK, Broder K, Iskander JK, Tm U, Mootrey G, et al. Prevention and control of influenza: recommendations of the advisory committee on immunization practices. Recomm Rep. 2008;57:1–60.
5.
6.
Khalafalla MM, Abdellatef E, Dafalla HM, Nassrallah AA, Aboul-Enein KM, Lightfoot DA, et al. Active principle from moringa oleifera lam leaves effective against two leukemias and a hepatocarcinoma. Afr J Biotechnol. 2010;9:8467–71.
7.
Mozaffari Nejad AS, Kamkar A, Giri A, Pourmahmoudi AA. Ethnobotany and folk medicinal uses of major trees and shrubs in northern Iran. J Med Plants Res. 2013;7:284–9.
8.
Amic D, Amie DD, Beslo D, Trinajstic N. Structural-radical scavenging activity relationship of flavonoids. Croatia Chemica Acta. 2003;76:55–61.
9.
Aqil F, Ahmad I, Mehmood Z. Antioxidant and free radical scavenging properties of twelve traditionally used Indian medicinal plants. Turk J Biol. 2006;30:177–83.
10.
Jalali H, Mozaffari Nejad AS, Ebadi AG, Laey G. Ethnobotany and Folk pharmaceutical properties of major trees or shrubs in northeast of Iran. Asian J Chem. 2009;21:5632–8.
11.
Street RA, Prinsloo G. Commercially important medicinal plants of South Africa: a review. Journal of Chemistry. 2013;2013:16.CrossRef
12.
Ruffa MJ, Wagner ML, Suriano M, Vicente C, Nadinic J, Pampuro S, et al. Inhibitory effect of medicinal herbs against RNA and DNA viruses. Antivir Chem Chemother. 2004;15:153–9.CrossRefPubMed
13.
14.
Hwang BS, Lee IK, Choi HJ, Yun BS. Anti-influenza activities of polyphenols from the medicinal mushroom Phellinus baumii. Bioorg Med Chem Lett. 2015;25:3256–60.CrossRefPubMed
15.
Zink T, Chaffin J. Herbal health products: what family physicians need to know. Am Fam Physician. 1998;58:1133–40.PubMed
16.
Milugo TK, Omosa LK, Ochanda JO, Owuor BO, Wamunyokoli FA, Oyugi JO, et al. Antagonistic effect of alkaloids and saponins on bioactivity in the quinine tree (Rauvolfia caffra sond.): further evidence to support biotechnology in traditional medicinal plants. BMC Complement Altern Med. 2013;13:285.CrossRefPubMedPubMedCentral
17.
Gwala PE. Anti-platelet aggregation activity of Rapanea melanophloeos -A Zulu medicinal plant, in Department of Biochemistry and Microbioogy. South Africa: University of Zululand; 2011. p. 111.
18.
Mehrbod P, Abdalla MA, Njoya EM, Ahmed AS, Fotouhi F, Farahmand B, et al. South African medicinal plant extracts active against influenza A virus. BMC Complement Altern Med. 2018;18:112.CrossRefPubMedPubMedCentral
19.
Karber G. 50% endpoint calculation archive for experimental pathology and. Pharmacology. 1931;162:480–3.
20.
Mehrbod P, Ideris A, Omar AR, Hair-Bejo M. Evaluation of antiviral effect of atorvastatin on H1N1 infection in MDCK cells. Afr J Microbiol Res. 2012;6:5715–9.
21.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65:55–63.CrossRefPubMed
22.
Mehrbod P, Motamed N, Tabatabaian M, Soleimani-Estyar R, Amini E, Shahidi M, et al. In vitro antiviral effect of “Nanosilver” on influenza virus. Daru. 2009;17:88–93.
23.
24.
25.
Chapman, Hall. Chapman-hall dictionary of natural products on CD-ROM, Chemical DataBase (2017).
26.
Chattopadhyay D, Sarkar MC, Chatterjee T, Sharma Dey R, Bag P, Chakraborti S, et al. Recent advancements for the evaluation of anti-viral activities of natural products. New Biotechnol. 2009;25:347–68.CrossRef
27.
Kobasa D, Jones SM, Shinya K, Kash JC, Copps J, Ebihara H, et al. Aberrant innate immune response in lethal infection of macaques with the 1918 influenza virus. Nature. 2007;445:319–23.CrossRefPubMed
28.
Piqueras B, Connolly J, Freitas H, Palucka AK, Banchereau J. Upon viral exposure mye-loid and plasmacytoid dendritic cells produce three waves of distinct chemokines to recruitimmune effectors. Blood. 2006;107:2613–8.CrossRefPubMedPubMedCentral
29.
Liang-Tzung L, Wen-Chan H, Chun-Ching L. Antiviral natural products and herbal medicines. J Tradit Complement Med. 2014;4:24–35.CrossRef
30.
Kumar S, Abhay KP. Chemistry and biological activities of flavonoids: an overview. Sci World J. 2013;2013:16.
31.
Kaul TN, Middleton E, Ogra PL. Antiviral effect of flavonoids on human viruses. J Med Virol. 1985;15:71–9.CrossRefPubMed
32.
Orhan DD, Özçelik B, Özgen S, Ergun F. Antibacterial, antifungal, and antiviral activities of some flavonoids. Microbiol Res. 2010;165:496–504.CrossRefPubMed
33.
Zandi K, Teoh BT, Sam SS, Wong PF, Mustafa MR, AbuBakar S. Antiviral activity of four types of bioflavonoid against dengue virus type-2. Virol J. 2011;8:560.CrossRefPubMedPubMedCentral
34.
Hafidh RR, Abdulamir AS, Jahanshiri F, Abas F, Abu Bakar F, Sekawi Z. Asia is the mine of natural antiviral products for public health. Open Complement Med J. 2009;1:58–68.
35.
Dayem AA, Choi HY, Kim YB, Cho SG. Antiviral effect of methylated flavonol isorhamnetin against influenza. PLoS One. 2015;10:e0121610.CrossRefPubMedCentral
36.
Hossain MK, Choi HY, Hwang JS, Dayem AA, Kim JH, Kim YB, et al. Antiviral activity of 3,4′-dihydroxyflavone on influenza a virus. J Microbiol. 2014;52:521–6.CrossRefPubMed
37.
Arena A, Bisignano G, Pavone B, Tomaino A, Bonina FP, Saija A, et al. Antiviral and immunomodulatory effect of a lyophilized extract of Capparis spinosa L. buds. Phytother Res. 2008;22:313–7.CrossRefPubMed
38.
Chiang LC, Ng LT, Chiang W, Chang MY, Lin CC. Immunomodulatory activities of flavonoids, monoterpenoids, triterpenoids, iridoid glycosides and phenolic compounds of Plantago species. Planta Med. 2003;69:600–4.CrossRefPubMed
39.
Aichour R, Charef N, Baghiani A, Arrar L. Immunomodulatory effects of Algerian caper. Int J Pharm Pharm Sci. 2016;8:51–4.
40.
Miles SL, McFarland M, Niles RM. Molecular and physiological actions of quercetin: need for clinical trials to assess its benefits in human disease. Nutr Rev. 2014;72:720–34.CrossRefPubMed
41.
Wu W, Li R, Li X, He J, Jiang S, Liu S, et al. Quercetin as an antiviral agent inhibits influenza a virus (IAV) entry. Viruses. 2016;8:6.CrossRef
42.
Choi HJ, Song JH, Park KS, Kwon DH. Inhibitory effects of quercetin 3-rhamnoside on influenza a virus replication. Eur J Pharm Sci. 2009;37:329–33.CrossRefPubMed
43.
Gannagé M, Dormann D, Albrecht R, Dengjel J, Torossi T, Rämer PC, et al. Matrix protein 2 of influenza a virus blocks autophagosome fusion with lysosomes. Cell Host Microbe. 2009;6:367–80.CrossRefPubMedPubMedCentral
44.
Nayak DP, Hui EKW, Barman S. Assembly and budding of influenza virus. Virus Res. 2004;106:147–65.CrossRefPubMed
45.
46.
Sun J, Dodd H, Moser EK, Sharma R, Braciale TJ. CD4+ T cell help and innate-derived IL-27 induce Blimp-1-dependent IL-10 production by antiviral CTLs. Nat Immunol. 2011;12:327–34.CrossRefPubMedPubMedCentral
47.
Cheung C, Poon L, Lau A, Luk W, Lau Y, Shortridge K, et al. Induction of proinflammatory cytokines in human macrophages by influenza a (H5N1) viruses: a mechanism for the unusual severity of human disease? Lancet. 2002;360:1831–7.CrossRefPubMed
48.
Swardfager W, Lanctôt K, Rothenburg L, Wong A, Cappell J, Herrmann N. Tumor necrosis factor alpha. Biol Psychiatry. 2010;68:930–41.CrossRefPubMed
Metadata
Title
Immunomodulatory properties of quercetin-3-O-α-L-rhamnopyranoside from Rapanea melanophloeos against influenza a virus
Authors
Parvaneh Mehrbod
Muna Ali Abdalla
Fatemeh Fotouhi
Masoumeh Heidarzadeh
Abimbola O. Aro
Jacobus N. Eloff
Lyndy J. McGaw
Folorunso O. Fasina
Publication date
01-12-2018
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2018
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-018-2246-1

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