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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2018

Open Access 01-12-2018 | Research article

Five traditional Nigerian Polyherbal remedies protect against high fructose fed, Streptozotocin-induced type 2 diabetes in male Wistar rats

Authors: O. E. Kale, O. B. Akinpelu, A. A. Bakare, F. O. Yusuf, R. Gomba, D. C. Araka, T. O. Ogundare, A. C. Okolie, O. Adebawo, O. Odutola

Published in: BMC Complementary Medicine and Therapies | Issue 1/2018

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Abstract

Background

This present study sought to assess the modulatory effects of five Nigerian traditional polyherbal in high fructose-fed, streptozotocin-induced (HF-STZ) Type 2 diabetes (T2D) in rats. T2D was achieved via fructose feeding (20%W/V) ad libitum for 2 weeks and streptozotocin (STZ, 40 mg/kg) (15th Day) intraperitoneally.

Methods

Seventy-two hours after STZ injection, fourty-eight diabetic rats were divided into eight of 6 rats/group: Diabetic normal untreated, glibenclamide (GBLI, 0.07 mL/kg) or yoyo (YB, 0.43), ruzu (RB, 0.08), fajik (FJB, 0.20), oroki (OB, 0.16), and fidson (FB, 0.43)/ mL/kg bitters respectively. Controls normal and diabetic untreated groups received intragastric carboxylmethylcellulose (CMC, 1 mL/kg) for eleven days.

Results

T2D was characterized in rats by an increased (p < 0.001–0.05) blood glucose levels (BGL), total cholesterol, triglycerides, low-density lipoprotein and alanine aminotransferase compared with control CMC group. Similarly, hepatic and pancreatic malondialdehyde (MDA) were increased by 180 and 97% respectively. Polyherbal treatments demonstrated efficacies on BGL as follow: YB (55.6%, 160.7 mg/dL); RB (59.7%, 145.2 mg/dL); FJB (59.8%, 243.4 mg/dL); OB (60.8%, 194.5 mg/dL) and FB (61.3%, 203.3 mg/dL) respectively by day 11 (versus GBLI, 65.1%) compared with control untreated diabetic rats. Also, elevated TC, LDL cholesterol, ALT were lowered (p < 0.05) by YB, FJB, and FB respectively in rats. YB, FJB, and OB lowered MDA levels in treated rats. Further, YB, RB, FJB and FB restored changes in liver, and pancreas histopathology. Predominant non-polar bioactive include oleic, hexadecanoic, octadecanoic among others following gas chromatography-mass spectrophotometry analyses.

Conclusion

Overall, these present results demonstrate anti-hyperglycemic potentials, although with cautions, of some polyherbal in T2D rats, which may, in part, be antioxidants mediated.
Literature
1.
Jordan SA, Cunningham DG, Marles RJ. Assessment of herbal medicinal products: challenges, and opportunities to increase the knowledge base for safety assessment. Toxicol Appl Pharmacol. 2010;243(2):198–216.CrossRefPubMed
2.
Keter LK, Mutiso PC. Ethnobotanical studies of medicinal plants used by traditional health practitioners in the management of diabetes in lower Eastern Province, Kenya. J Ethnopharmacology. 2012;139(1):74–80.CrossRef
3.
Boden G, Shulman GI. Free fatty acids in obesity and type 2 diabetes: defining their role in the development of insulin resistance and β-cell dysfunction. Eur J Clin Investig. 2002;32(s3):14–23.CrossRef
4.
Rodriguez-Fragoso L, Reyes-Esparza J, Burchiel SW, Herrera-Ruiz D, Torres E. Risks and benefits of commonly used herbal medicines in Mexico. Toxicol Appl Pharmacol. 2008;227(1):125–35.CrossRefPubMed
5.
Murad MH, Coto-Yglesias F, Wang AT, Sheidaee N, Mullan RJ, Elamin MB, Montori VM. Drug-induced hypoglycemia: a systematic review. J Clin Endocrin Metabolism. 2009;94(3):741–5.CrossRef
6.
Li S, Zhang B, Zhang N. Network target for screening synergistic drug combinations with application to traditional Chinese medicine. BMC Syst Biol. 2011;5(1):S10.CrossRefPubMedPubMedCentral
7.
Adeyemi OS, Fambegbe M, Daniyan OR, Nwajei I. Yoyo bitters, a polyherbal formulation influenced some biochemical parameters in Wistar rats. J Basic Clin Physiol Pharmacol. 2012;23(4):135–8.PubMed
8.
9.
Emordi JE, Agbaje EO, Oreagba IA, Iribhogbe OI. Antidiabetic and hypolipidemic activities of hydroethanolic root extract of Uvaria chamae in streptozotocin induced diabetic albino rats. BMC Complement Altern Med. 2016;16(1):468.CrossRefPubMedPubMedCentral
10.
Stojanović G, Golubović T, Palić R. Acinos species: chemical composition, antimicrobial and antioxidative activity. J Med Plants Res. 2009;3(13):1240–7.
11.
Sharma P, Kharkwal AC, Kharkwal H, Abdin MZ, Varma A. A review on pharmacological properties of Aloe vera. Int J Pharm Sci Rev Res. 2014;29(2):31–7.
12.
Radha MH, Laxmipriya NP. Evaluation of biological properties and clinical effectiveness of Aloe vera: a systematic review. J traditional complementary med. 2015;5(1):21–6.CrossRef
13.
Cefalu WT, Ribnicky D. Modulation of insulin action by botanical therapeutics. Obesity Weight Management. 2009;5(6):277–81.CrossRef
14.
Mohamed AE, Abdel-Aziz AF, El-Sherbiny EM, Mors R. Anti-diabetic effect of Aloe vera juice and evaluation of thyroid function in female diabetic rats. Biosci Res. 2009;6:28–34.
15.
Odugbemi TO, Akinsulire OR, Aibinu IE, Fabeku PO. Medicinal plants useful for malaria therapy in Okeigbo, Ondo state, Southwest Nigeria. Afr J Tradit Complement Altern Med. 2007;4(2):191–8.
16.
Benavente-Garcia O, Castillo J. Update on uses and properties of citrus flavonoids: new findings in anticancer, cardiovascular, and anti-inflammatory activity. J Agric Food Chem. 2008;56(15):6185–205.CrossRefPubMed
17.
Calzada F, Yépez-Mulia L, Aguilar A. In vitro susceptibility of Entamoeba histolytica and Giardia lamblia to plants used in Mexican traditional medicine for the treatment of gastrointestinal disorders. J Ethnopharmacol. 2006;108(3):367–70.CrossRefPubMed
18.
Nabavi SF, Di Lorenzo A, Izadi M, Sobarzo-Sánchez E, Daglia M, Nabavi SM. Antibacterial effects of cinnamon: from farm to food, cosmetic and pharmaceutical industries. Nutrients. 2015;7(9):7729–48.CrossRefPubMedPubMedCentral
19.
Sofidiya MO, Oduwole B, Bamgbade E, Odukoya O, Adenekan S. Nutritional composition and antioxidant activities of Curculigo pilosa (Hypoxidaceae) rhizome. Afr J Biotechnol. 2011;10(75):17275–81.
20.
Murali R, Saravanan R. Antidiabetic effect of d-limonene, a monoterpene in streptozotocin-induced diabetic rats. Biomedicine & Preventive Nutrition. 2012;2(4):269–75.CrossRef
21.
Syamasundar KV, Singh B, Thakur RS, Husain A, Yoshinobu K, Hiroshi H. Antihepatotoxic principles of Phyllanthus niruri herbs. J Ethnopharmacol. 1985;14(1):41–4.CrossRefPubMed
22.
Bansal J, Kumar N, Malviya R, Sharma PK. Hepatoprotective models and various natural product used in Hepatoprotective agents: a review. Pharmacognosy Communications. 2014;4(3):2.CrossRef
23.
Balachandran P, Govindarajan R. Ayurvedic drug discovery. Expert Opin Drug Discovery. 2007;2(12):1631–52.CrossRef
24.
25.
Ezuruike UF, Prieto JM. The use of plants in the traditional management of diabetes in Nigeria: pharmacological and toxicological considerations. J Ethnopharmacol. 2014;155(2):857–924.CrossRefPubMed
26.
27.
Kilkenny C, Browne WJ, Cuthill IC, Emerson M, Altman DG. Improving bioscience research reporting: the ARRIVE guidelines for reporting animal research. PLoS Biol. 2010;8(6):e1000412.CrossRefPubMedPubMedCentral
28.
Onyeaghala AA, Omotosho IO, Shivashankara AR. Cytotoxicity of various fractions of compounds extracted from Yoyo bitters on human cervical Cancer cells. European J Med Plants. 2015;7(2):46–58.CrossRef
29.
Proestos C, Sereli D, Komaitis M. Determination of phenolic compounds in aromatic plants by RP-HPLC and GC-MS. Food Chem. 2006;95(1):44–52.CrossRef
30.
31.
Wilson R, Islam M. (2012). Fructose-fed Streptozotocin injected rat; an alternative model for type 2 diabetes. Pharm.
32.
Reitman S, Frankel SA. Colorimetric method for the determination of serum glutamate oxaloacetate and pyruvate transaminases. Am J Clin Pathol. 1957;28:56–63.CrossRefPubMed
33.
Roy AV. Rapid method for determining alkaline phosphatase activity in serum with thymolphthalein monophosphate. Clin Chem. 1970;16(5):431–6.PubMed
34.
Trinder P. Quantitative determination of triglyceride using GPO-PAP method. Annals Clin Biochem. 1969;6:24–7.CrossRef
35.
Fossati P, Prencipe L, Berti G. Enzymic creatinine assay: a new colorimetric method based on hydrogen peroxide measurement. Clin Chem. 1983;29(8):1494–6.PubMed
36.
Warnick GR, Albers JJ. A comprehensive evaluation of the heparinmanganese precipitation procedure for estimating high density lipoprotein cholesterol. J Lipid Res. 1978;19:65–76.PubMed
37.
Friedewald WT, Levy RI, Fredrickson DS. Estimation of the concentration of low-density lipoprotein cholesterol in plasma, without use of the preparative ultracentrifuge. Clin Chem. 1972;18:499–502.PubMed
38.
Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. J Lab Clin Med. 1963;61:882–8.PubMed
39.
Varshney R, Kale RK. Effect of calmodulin antagonist on radiation induced lipid peroxidation in microsomes. Inter J Radiation Biol. 1990;58(5):733–43.CrossRef
40.
Shaw JE, Sicree RA, Zimmet PZ. Global estimates of the prevalence of diabetes for 2010 and 2030. Diabetes Res Clin Pract. 2010;87(1):4–14.CrossRefPubMed
41.
Whiting DR, Guariguata L, Weil C, Shaw J. IDF diabetes atlas: global estimates of the prevalence of diabetes for 2011 and 2030. Diabetes Res Clin Pract. 2011;94(3):311–21.CrossRefPubMed
42.
Gbolade AA. Inventory of antidiabetic plants in selected districts of Lagos state Nigeria. J Ethnopharmacology. 2009;121(1):135–9.CrossRef
43.
Mohan CG, Viswanatha GL, Savinay G, Rajendra CE, Halemani PD. 1, 2, 3, 4, 6 Penta-O-galloyl-β-d-glucose, a bioactivity guided isolated compound from Mangifera indica inhibits 11β-HSD-1 and ameliorates high fat diet-induced diabetes in C57BL/6 mice. Phytomedicine. 2013;20(5):417–26.CrossRefPubMed
44.
Sangeetha KN, Sujatha S, Muthusamy VS, Anand S, Nithya N, Velmurugan D, Lakshmi BS. 3β-taraxerol of Mangifera indica, a PI3K dependent dual activator of glucose transport and glycogen synthesis in 3T3-L1 adipocytes. Biochimica et Biophysica Acta (BBA)-General Subjects. 2010;1800(3):359–66.CrossRef
45.
46.
Sreekeesoon DP, Mahomoodally MF. Ethnopharmacological analysis of medicinal plants and animals used in the treatment and management of pain in Mauritius. J Ethnopharmacol. 2014;157:181–200.CrossRefPubMed
47.
Farrar JL, Hartle DK, Hargrove JL, Greenspan P. A novel nutraceutical property of select sorghum (Sorghum bicolor) brans: inhibition of protein glycation. Phytother Res. 2008;22(8):1052–6.CrossRefPubMed
48.
Patel DK, Kumar R, Laloo D, Hemalatha S. Natural medicines from plant source used for therapy of diabetes mellitus: an overview of its pharmacological aspects. Asian Pacific J Tropical Disease. 2012;2(3):239–50.CrossRef
49.
Soladoye MO, Chukwuma EC, Owa FP. An ‘Avalanche’of plant species for the traditional cure of diabetes mellitus in south-western Nigeria. J Nat Prod Plant Resour. 2012;2(1):60–72.
50.
Boaduo NKK, Katerere D, Eloff JN, Naidoo V. Evaluation of six plant species used traditionally in the treatment and control of diabetes mellitus in South Africa using in vitro methods. Pharm Biol. 2014;52(6):756–61.CrossRefPubMed
51.
Tuhin RH, Begum MM, Rahman MS, Karim R, Begum T, Ahmed SU, Begum R, Mostofa R, Hossain A, Abdel-Daim M, Begum R. Wound healing effect of Euphorbia hirta Linn.(Euphorbiaceae) in alloxan induced diabetic rats. BMC complementary and alternative medicine. 2017;17(1):423.CrossRefPubMedPubMedCentral
52.
Brownlee M. Biochemistry and molecular cell biology of diabetic complications. Nature. 2001;414(6865):813–20.CrossRefPubMed
53.
Rahimi R, Nikfar S, Larijani B, Abdollahi M. A review on the role of antioxidants in the management of diabetes and its complications. Biomed Pharmacother. 2005;59(7):365–73.CrossRefPubMed
Metadata
Title
Five traditional Nigerian Polyherbal remedies protect against high fructose fed, Streptozotocin-induced type 2 diabetes in male Wistar rats
Authors
O. E. Kale
O. B. Akinpelu
A. A. Bakare
F. O. Yusuf
R. Gomba
D. C. Araka
T. O. Ogundare
A. C. Okolie
O. Adebawo
O. Odutola
Publication date
01-12-2018
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2018
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-018-2225-6

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