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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2018

Open Access 01-12-2018 | Research article

Zanthoxylum armatum DC extracts from fruit, bark and leaf induce hypolipidemic and hypoglycemic effects in mice- in vivo and in vitro study

Authors: Fiaz Alam, Qazi Najam us Saqib, Mohammad Ashraf

Published in: BMC Complementary Medicine and Therapies | Issue 1/2018

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Abstract

Background

Zanthoxylum armatum DC is an important medicinal plant of south East Asia, and has been used to treat various ailments in traditional medicine including diabetes. This study investigated the in vitro and in vivo antidiabetic and biochemical effects of extracts of Z. armatum in mice.

Method

The extracts of fruit, bark and leaf from Z. armatum were tested for α-glucosidase inhibition activity. Albino mice of either sex weighing (26–30 g) assigned into groups. Diabetes was induced by IP injection of alloxan monohydrate (150 mg/kg). The extracts (500 mg/kg) and standard (Glibenclamide 10 mg/kg) were administered to mice for 15 days. Serum biochemical parameters were monitored for the period of study.

Results

The leaves and bark extracts showed maximum α-glucosidase inhibition (96.61 ± 2.13 and 93.58 ± 2.31% respectively). The extracts treated and the standard treated groups showed significant decrease in the fasting blood glucose levels compared to diabetic control. The effect was more pronounced in mice treated with leaves extract. In the in vivo studies body weights of diabetic mice treated with Z. armatum extracts and the standard did not reduced to extent as observed in diabetic control and this difference was significant (p < 0.05). There was a significance (p < 0.001) improvement in blood hemoglobin, urea, creatinine, cholesterol, and triglycerides of the extracts treated diabetic mice. The extracts showed hypolipidemic effect by reducing the LDL level. The extracts produced no prominent changes in proteins levels.

Conclusion

It can be concluded that Z. armatum extracts showed excellent antidiabetic potential in vivo and in vitro and could be considered for further appraisal in clinical assessment and drug development.
Literature
1.
Jadhav R, Puchchakayala G. Hypoglycemic and antidiabetic activity of flavonoids: Boswellic acid, ellagic acid, quercetin, rutin on streptozotocin-nicotinamide induced type 2 diabetic rats. Group. 2012;1:100g.
2.
Ceylan S, Azal Ö, Taşlipinar A, Türker T, Açikel CH, Gulec M. Complementary and alternative medicine use among Turkish diabetes patients. Compl Ther Med. 2009;17(2):78–83.CrossRef
3.
World Health Organization. Annex, II-Guidelines for the Assessment of Herbal Medicines (WHO Technical Report Series No. 863). Geneva; 1996.
4.
Shruthi A, Latha K, Vagdevi H, Pushpa B, Shwetha C. Anti-diabetic activity of the leaves extracts of Wrightia Tinctoria on alloxan induced diabetic rats. J Chem & Pharm Res. 2012;4(6):3125–8.
5.
Grover J, Yadav S, Vats V. Medicinal plants of India with anti-diabetic potential. J Ethnopharmacol. 2002;81(1):81–100.CrossRefPubMed
6.
Eddouks M, Lemhadri A, Michel J-B. Caraway and caper: potential anti-hyperglycaemic plants in diabetic rats. J Ethnopharmacol. 2004;94(1):143–8.CrossRefPubMed
7.
Musabayane C, Bwititi P, Ojewole J. Effects of oral administration of some herbal extracts on food consumption and blood glucose levels in normal and streptozotocin-treated diabetic rats. Methods Find Exp Clin Pharmacol. 2006;28(4):223–8.CrossRefPubMed
8.
Collier E, Watkinson A, Cleland CF, Roth J. Partial purification and characterization of an insulin-like material from spinach and Lemna gibba G3. J Biol Chem. 1987;262(13):6238–47.PubMed
9.
Alarcon-Aguilara F, Roman-Ramos R, Perez-Gutierrez S, Aguilar-Contreras A, Contreras-Weber C, Flores-Saenz J. Study of the anti-hyperglycemic effect of plants used as antidiabetics. J Ethnopharmacol. 1998;61(2):101–10.CrossRefPubMed
10.
Mi B, Jelani G, Ahmad I. Leaf, Stem Bark And fruit anatomy of Zanthoxylum armatum dc.(Rutaceae). Pak J Bot. 2014;46(4):1343–9.
11.
Singh TP, Singh OM. Phytochemical and pharmacological profile of Zanthoxylum armatum DC.—an overview. Indian J Nat Prod Res. 2011;2(3):275–85.
12.
Rynjah CV, Devi NN, Khongthaw N, Syiem D, Majaw S. Evaluation of the antidiabetic property of aqueous leaves extract of Zanthoxylum armatum DC. Using in vivo and in vitro approaches. J Trad Compl Med. 2018;8(1):134–40.CrossRef
13.
Karki H, Upadhayay K, Pal H, Singh R. Antidiabetic potential of Zanthoxylum armatum bark extract on streptozotocin-induced diabetic rats. Intern J Green Pharm (IJGP). 2014;8(2):77.CrossRef
14.
Matsui T, Yoshimoto C, Osajima K, Oki T, Osajima Y. In vitro survey of a-glucosidase inhibitory food components. Biosci Biotechnol Biochem. 1996;60(12):2019–22.CrossRefPubMed
15.
Weir GC, Bonner-Weir S. Five stages of evolving beta-cell dysfunction during progression to diabetes. Diabetes. 2004;53(suppl 3):S16–21.CrossRefPubMed
16.
Ou Y, Lin L, Yang X, Pan Q, Cheng X. Antidiabetic potential of phycocyanin: effects on KKAy mice. Pharm Biol. 2013;51(5):539–44.CrossRefPubMed
17.
Council NR. Guide for the care and use of laboratory animals. Washington DC: National Academy Press; 1996.
18.
Phuong M, Ali B, Aziz E, Abdellatif S, Yahia C, Pierre S. The petroleum ether extract of Nigella sativa exerts lipid lowering and insulin-sensitizing action in the rats. J Ethnopharmacol. 2004;94:251–9.CrossRef
19.
Burtis CA, Ashwood ER, Bruns DE. Tietz textbook of clinical chemistry and molecular diagnostics. Elsevier Health Sciences. 2012;
20.
Thomas L, Whicher JT, Andert SE: Clinical laboratory diagnostics: use and assessment of clinical laboratory results: TH-books; 1998.
21.
Zaia DA, Marques FR, Zaia CT. Spectrophotometric determination of total proteins in blood plasma: a comparative study among dye-binding methods. Braz Arch Biol Technol. 2005;48(3):385–8.CrossRef
22.
Burstein M, Scholnick H, Morfin R. Rapid method for the isolation of lipoproteins from human serum by precipitation with polyanions. J Lipid Res. 1970;11(6):583–95.PubMed
23.
Salimifar M, Fatehi-Hassanabad Z, Fatehi M. A review on natural products for controlling type 2 diabetes with an emphasis on their mechanisms of actions. Curr Diab Rev. 2013;9(5):402–11.CrossRef
24.
Shapiro K, Gong WC. Natural products used for diabetes. J Amer Pharm Assoc. 2002;42(2):217–26.
25.
Ya M, Umamageswari M, Karthikeyan T. Evaluation of Antihyperglycemic activity of aqueous extract of leaves of. Solanum Nigrum. Int J Pharm Bio Sci. 2012:312–9.
26.
Karan SK, Mishra SK, Pal D, Mondal A. Isolation of β-sitosterol and evaluation of antidiabetic activity of Aristolochia indica in alloxan-induced diabetic mice with a reference to in-vitro antioxidant activity. J Med Plants Res. 2012;6(7):1219–23.
27.
Prisilla DH, Balamurugan R, Shah HR. Antidiabetic activity of methanol extract of Acorus calamus in STZ induced diabetic rats. Asian Pac J Trop Biomed. 2012;2(2):S941–6.CrossRef
28.
Pari L, Latha M. Protective role of Scoparia dulcis plant extract on brain antioxidant status and lipidperoxidation in STZ diabetic male Wistar rats. BMC Compl Altern Med. 2004;4(1):16.CrossRef
29.
Sezik E, Aslan M, Yesilada E, Ito S. Hypoglycaemic activity of Gentiana olivieri and isolation of the active constituent through bioassay-directed fractionation techniques. Life Sci. 2005;76(11):1223–38.CrossRefPubMed
30.
Slei A. Effects of Zanthoxylum zanthoxyloides leaves on blood glucose, lipid profile and some liver enzymes in alloxan induced diabetic rats. Intern J Sci Nat. 2012;3(3):497–501.
31.
Gao H, Huang Y-N, Gao B, Xu P-Y, Inagaki C, Kawabata J. α-glucosidase inhibitory effect by the flower buds of Tussilago farfara L. Food Chem. 2008;106(3):1195–201.CrossRef
32.
33.
Sikarwar M, Patil M. Antidiabetic activity of Pongamia pinnata leaf extracts in alloxan-induced diabetic rats. Intern J Ayur Res. 2010;1(4):199.CrossRef
34.
Luo Q, Cai Y, Yan J, Sun M, Corke H. Hypoglycemic and hypolipidemic effects and antioxidant activity of fruit extracts from Lycium barbarum. Life Sci. 2004;76(2):137–49.CrossRefPubMed
35.
Koneri RB, Samaddar S, Ramaiah CT. Antidiabetic activity of a triterpenoid saponin isolated from Momordica cymbalaria Fenzl. Indian J Exp Biol. 2014;52:46–52.PubMed
36.
Tiong SH, Looi CY, Hazni H, Arya A, Paydar M, Wong WF, Cheah S-C, Mustafa MR, Awang K. Antidiabetic and antioxidant properties of alkaloids from Catharanthus roseus (L.) G. Don. Molecules. 2013;18(8):9770–84.CrossRefPubMed
37.
Salib JY, Michael HN, Eskande EF. Anti-diabetic properties of flavonoid compounds isolated from Hyphaene thebaica epicarp on alloxan induced diabetic rats. Pharm Res. 2013;5(1):22.
38.
Oboh G, Ogunsuyi OB, Ogunbadejo MD, Adefegha SA. Influence of gallic acid on α-amylase and α-glucosidase inhibitory properties of acarbose. J Food Drug Anal. 2016;24(3):627–34.CrossRefPubMed
39.
Alam F. QN US: Pharmacognostic study and development of quality control parameters for fruit, bark and leaf of Zanthoxylum armatum (Rutaceae). Anc Sci Life. 2015;34(3):148.
40.
Gerbes A, Gülberg V, Bilzer M, Vogeser M. Evaluation of serum cystatin C concentration as a marker of renal function in patients with cirrhosis of the liver. Gut. 2002;50(1):106–10.CrossRefPubMedPubMedCentral
41.
Nabeel MA, Kathiresan K, Manivannan S. Antidiabetic activity of the mangrove species Ceriops decandra in alloxan-induced diabetic rats. J Diab. 2010;2(2):97–103.CrossRef
Metadata
Title
Zanthoxylum armatum DC extracts from fruit, bark and leaf induce hypolipidemic and hypoglycemic effects in mice- in vivo and in vitro study
Authors
Fiaz Alam
Qazi Najam us Saqib
Mohammad Ashraf
Publication date
01-12-2018
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2018
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-018-2138-4

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